HIF1A

Hypoxia-inducible factor 1-alpha, also known as HIF-1-alpha, is a subunit of a heterodimeric transcription factor hypoxia-inducible factor 1 (HIF-1) that is encoded by the HIF1A gene.[5][6][7] It is a basic helix-loop-helix PAS domain containing protein, and is considered as the master transcriptional regulator of cellular and developmental response to hypoxia.[8][9] The dysregulation and overexpression of HIF1A by either hypoxia or genetic alternations have been heavily implicated in cancer biology, as well as a number of other pathophysiologies, specifically in areas of vascularization and angiogenesis, energy metabolism, cell survival, and tumor invasion.[7][10] Two other alternative transcripts encoding different isoforms have been identified.[7]

HIF1A
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1H2K, 1H2L, 1H2M, 1L3E, 1L8C, 1LM8, 1LQB, 2ILM, 3HQR, 3HQU, 4AJY, 4H6J
Identifiers
Aliases	HIF1A, HIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78, hypoxia inducible factor 1 alpha subunit, hypoxia inducible factor 1 subunit alpha, HIF-1α
External IDs	OMIM: 603348 MGI: 106918 HomoloGene: 1171 GeneCards: HIF1A
Gene location (Human) Chr. Chromosome 14 (human)[1] Band 14q23.2 Start 61,695,513 bp[1] End 61,748,259 bp[1]
Gene location (Mouse) Chr. Chromosome 12 (mouse)[2] Band 12 C3|12 31.99 cM Start 73,901,375 bp[2] End 73,947,530 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • protein dimerization activity • GO:0001131, GO:0001151, GO:0001130, GO:0001204 DNA-binding transcription factor activity • GO:0001077, GO:0001212, GO:0001213, GO:0001211, GO:0001205 DNA-binding transcription activator activity, RNA polymerase II-specific • histone deacetylase binding • transcription factor binding • enzyme binding • GO:0001948 protein binding • histone acetyltransferase binding • protein kinase binding • Hsp90 protein binding • sequence-specific DNA binding • DNA binding • nuclear hormone receptor binding • transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding • ubiquitin protein ligase binding • protein heterodimerization activity • E-box binding • p53 binding • protein domain specific binding • GO:0001200, GO:0001133, GO:0001201 DNA-binding transcription factor activity, RNA polymerase II-specific • RNA polymerase II regulatory region sequence-specific DNA binding Cellular component • cytoplasm • cytosol • cell nucleus • nuclear speck • motile cilium • transcription factor complex • RNA polymerase II transcription factor complex • axon cytoplasm • nucleoplasm • nuclear body • macromolecular complex • nuclear chromatin Biological process • negative regulation of neuron apoptotic process • B-1 B cell homeostasis • regulation of transforming growth factor beta2 production • muscle cell cellular homeostasis • outflow tract morphogenesis • negative regulation of ossification • heart looping • negative regulation of TOR signaling • blood vessel development • hemoglobin biosynthetic process • angiogenesis • positive regulation of chemokine-mediated signaling pathway • positive regulation of insulin secretion involved in cellular response to glucose stimulus • positive regulation of hormone biosynthetic process • negative regulation of mesenchymal cell apoptotic process • regulation of aerobic respiration • positive regulation of neuroblast proliferation • dopaminergic neuron differentiation • lactate metabolic process • regulation of transcription, DNA-templated • blood vessel morphogenesis • positive regulation of erythrocyte differentiation • glucose homeostasis • vascular endothelial growth factor production • regulation of thymocyte apoptotic process • positive regulation of epithelial cell migration • negative regulation of thymocyte apoptotic process • transcription, DNA-templated • positive regulation of transcription, DNA-templated • axonal transport of mitochondrion • positive regulation of macroautophagy • cartilage development • positive regulation of nitric-oxide synthase activity • regulation of transcription from RNA polymerase II promoter in response to hypoxia • lactation • negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway • positive regulation of pri-miRNA transcription from RNA polymerase II promoter • digestive tract morphogenesis • cell differentiation • neural fold elevation formation • positive regulation of autophagy • retina vasculature development in camera-type eye • positive regulation of receptor biosynthetic process • collagen metabolic process • embryonic placenta development • negative regulation of apoptotic process • positive regulation of angiogenesis • regulation of glycolytic process • epithelial to mesenchymal transition • cerebral cortex development • regulation of gene expression • positive regulation of chemokine production • intestinal epithelial cell maturation • GO:0048552 regulation of catalytic activity • positive regulation of autophagy of mitochondrion • cardiac ventricle morphogenesis • response to muscle activity • epithelial cell differentiation involved in mammary gland alveolus development • visual learning • positive regulation of vascular endothelial growth factor receptor signaling pathway • negative regulation of bone mineralization • negative regulation of growth • response to hypoxia • positive regulation of endothelial cell proliferation • regulation of transcription from RNA polymerase II promoter in response to oxidative stress • iris morphogenesis • mRNA transcription from RNA polymerase II promoter • hypoxia-inducible factor-1alpha signaling pathway • vasculature development • regulation of cell proliferation • neural crest cell migration • embryonic hemopoiesis • connective tissue replacement involved in inflammatory response wound healing • positive regulation of transcription from RNA polymerase II promoter in response to hypoxia • negative regulation of reactive oxygen species metabolic process • positive regulation of vascular endothelial growth factor production • elastin metabolic process • positive regulation of glycolytic process • oxygen homeostasis • signal transduction • positive regulation of transcription from RNA polymerase II promoter • cellular iron ion homeostasis • camera-type eye morphogenesis • cellular response to hypoxia • cellular response to interleukin-1 • transcription from RNA polymerase II promoter • protein deubiquitination • post-translational modification • response to iron ion • negative regulation of gene expression • positive regulation of blood vessel endothelial cell migration • positive regulation of gene expression • cytokine-mediated signaling pathway • regulation of transcription from RNA polymerase II promoter • protein ubiquitination Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	3091	15251
Ensembl	ENSG00000100644	ENSMUSG00000021109
UniProt	Q16665	Q61221
RefSeq (mRNA)	NM_181054 NM_001243084 NM_001530	NM_010431 NM_001313919 NM_001313920
RefSeq (protein)	NP_001230013 NP_001521 NP_851397 NP_001521.1	NP_001300848 NP_001300849 NP_034561
Location (UCSC)	Chr 14: 61.7 – 61.75 Mb	Chr 12: 73.9 – 73.95 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Structure

HIF1 is a heterodimeric basic helix-loop-helix structure[11] that is composed of HIF1A, the alpha subunit (this protein), and the aryl hydrocarbon receptor nuclear translocator (Arnt), the beta subunit. HIF1A contains a basic helix-loop-helix domain near the C-terminal, followed by two distinct PAS (PER-ARNT-SIM) domains, and a PAC (PAS-associated C-terminal) domain.[8][6] The HIF1A polypeptide also contains a nuclear localization signal motif, two transactivating domains CTAD and NTAD, and an intervening inhibitory domain (ID) that can repress the transcriptional activities of CTAD and NTAD.[12] There are a total of three HIF1A isoforms formed by alternative splicing, however isoform1 has been chosen as the canonical structure, and is the most extensively studied isoform in structure and function.[13][14]

Gene and expression

The human HIF1A gene encodes for the alpha subunit, HIF1A of the transcription factor hypoxia-inducible factor (HIF1) [15] . Its protein expression level can be measured by antibodies against HIF-1-alpha through various biological detection methods including western blot or immunostaining.[16] HIF1A expression level is dependent on its GC-rich promoter activation.[17] In most cells, HIF1A gene is constitutively expressed in low levels under normoxic conditions, however, under hypoxia, HIF1A transcription is often significantly upregulated.[17][18][19][20][21][22] Typically, oxygen-independent pathway regulates protein expression, and oxygen-dependent pathway regulates degradation.[10] In hypoxia-independent ways, HIF1A expression may be upregulated through a redox-sensitive mechanism.[23]

Function

Nobel Prize in Physiology/ Medicine 2019: Cellular Oxygen Sensing and Adaption by Hif-alpha

The transcription factor HIF-1 plays an important role in cellular response to systemic oxygen levels in mammals.[24][25] HIF1A activity is regulated by a host of post-translational modifications: hydroxylation, acetylation, and phosphorylation.[26] HIF-1 is known to induce transcription of more than 60 genes, including VEGF and erythropoietin that are involved in biological processes such as angiogenesis and erythropoiesis, which assist in promoting and increasing oxygen delivery to hypoxic regions.[10][27][26] HIF-1 also induces transcription of genes involved in cell proliferation and survival, as well as glucose and iron metabolism.[26] In accordance with its dynamic biological role, HIF-1 responds to systemic oxygen levels by undergoing conformational changes, and associates with HRE regions of promoters of hypoxia-responsive genes to induce transcription.[28][29][30][31][32]

HIF1A stability, subcellular localization, as well as transcriptional activity are especially affected by oxygen level. The alpha subunit forms a heterodimer with the beta subunit. Under normoxic conditions, VHL-mediated ubiquitin protease pathway rapidly degrades HIF1A; however, under hypoxia, HIF1A protein degradation is prevented and HIF1A levels accumulate to associate with HIF1B to exert transcriptional roles on target genes [33][34] Enzymes prolyl hydroxylase (PHD) and HIF prolyl hydroxylase (HPH) are involved in specific post-translational modification of HIF1A proline residues (P402 and P564 within the ODD domain), which allows for VHL association with HIF1A.[32] The enzymatic activity of oxygen sensor dioxygenase PHD is dependent on oxygen level as it requires oxygen as one of its main substrates to transfer to the proline residue of HIF1A.[29][35] The hydroxylated proline residue of HIF1A is then recognized and buried in the hydrophobic core of von Hippel-Lindau tumor suppressor protein (VHL), which itself is part of a ubiquitin ligase enzyme.[36][37] The hydroxylation of HIF1A proline residue also regulates its ability to associate with co-activators under hypoxia.[38][39] Function of HIF1A gene can be effectively examined by siRNA knockdown based on an independent validation.[40]

Repair and regeneration

In normal circumstances after injury HIF1A is degraded by prolyl hydroxylases (PHDs). In June 2015, scientists found that the continued up-regulation of HIF1A via PHD inhibitors regenerates lost or damaged tissue in mammals that have a repair response; and the continued down-regulation of HIF1A results in healing with a scarring response in mammals with a previous regenerative response to the loss of tissue. The act of regulating HIF1A can either turn off, or turn on the key processes of mammalian regeneration.[41][42] One such regenerative process in which HIF1A is involved is peripheral nerve regeneration. Following axon injury, HIF1A activates VEGFA to promote regeneration and functional recovery.[43][44]

Regulation

HIF1A abundance (and its subsequent activity) is regulated transcriptionally in an NF-κB-dependent manner.[45] In addition, the coordinated activity of the prolyl hydroxylases (PHDs) maintain the appropriate balance of HIF1A protein in the post-translation phase.[46]

PHDs rely on iron among other molecules to hydroxylate HIF1A; as such, iron chelators such as desferrioxamine (DFO) have proven successful in HIF1A stabilization.[47] HBO (Hyperbaric oxygen therapy) and HIF1A imitators such as cobalt chloride have also been successfully utilized.[47]

Factors increasing HIF1A[48]

• Modulator of Degradation:
  • Oxygen-Dependent:
    • EPF UCP (degrades pHVL)
    • VDU2 (de-ubiquitinates HIF1A)
    • SUMOylation (via RSUME)
    • DeSUMOylation ( via SENP1)
  • Oxygen-independent:
    • Calcineurin A ( Ca2+-dependent via RACK1)
• Modulators of translation:
  • RNA-binding proteins, PTB, and HuR
  • PtdIns3K and MAPK pathways
  • IRES-mediated translation
  • calcium signaling
  • miRNAs

Factors decreasing HIF1A[48]

• Modulator of Degradation:
  • Oxygen-Dependent:
    • PHD, VHL, OS-9 and SSAT2
    • SUMOylation
  • Oxygen-independent
    • RACK1 and SSAT1
    • GSK3β
    • FOXO4
• Modulators of translation:
  • Calcium signaling
  • miRNAs

Role in cancer

HIF1A is overexpressed in many human cancers.[49][50] HIF1A overexpression is heavily implicated in promoting tumor growth and metastasis through its role in initiating angiogenesis and regulating cellular metabolism to overcome hypoxia.[51] Hypoxia promotes apoptosis in both normal and tumor cells.[52] However, hypoxic conditions in tumor microenvironment especially, along with accumulation of genetic alternations often contribute to HIF1A overexpression.[10]

Significant HIF1A expression has been noted in most solid tumors studied, which include cancers of the gastric, colon, breast, pancreas, kidneys, prostate, ovary, brain, and bladder.[53][50][49] Clinically, elevated HIF1A levels in a number of cancers, including cervical cancer, non-small-cell lung carcinoma, breast cancer (LV-positive and negative), oligodendroglioma, oropharyngeal cancer, ovarian cancer, endometrial cancer, esophageal cancer, head and neck cancer, and stomach cancer, have been associated with aggressive tumor progression, and thus has been implicated as a predictive and prognostic marker for resistance to radiation treatment, chemotherapy, and increased mortality.[10][54][55][56][57][53]HIF1A expression may also regulate breast tumor progression. Elevated HIF1A levels may be detected in early cancer development, and have been found in early ductal carcinoma in situ, a pre-invasive stage in breast cancer development, and is also associated with increased microvasculature density in tumor lesions.[58] Moreover, despite histologically-determined low-grade, lymph-node negative breast tumor in a subset of patients examined, detection of significant HIF1A expression was able to independently predict poor response to therapy.[51] Similar findings have been reported in brain cancer and ovarian cancer studies as well, and suggest at regulatory role of HIF1A in initiating angiogenesis through interactions with pro-angiogenic factors such as VEGF.[57][59] Studies of glioblastoma multiforme show striking similarity between HIF1A expression pattern and that of VEGF gene transcription level.[60][61] In addition, high-grade glioblastoma multiform tumors with high VEGF expression pattern, similar to breast cancer with HIF1A overexpression, display significant signs of tumor neovascularization.[62] This further suggests the regulatory role of HIF1A in promoting tumor progression, likely through hypoxia-induced VEGF expression pathways.[61]

[53] HIF1A overexpression in tumors may also occur in a hypoxia-independent pathway. In hemangioblastoma, HIF1A expression is found in most cells sampled from the well-vascularized tumor.[63] Although in both renal carcinoma and hemangioblastoma, the von Hippel-Lindau gene is inactivated, HIF1A is still expressed at high levels.[59][63][49] In addition to VEGF overexpression in response elevated HIF1A levels, the PI3K/AKT pathway is also involved in tumor growth. In prostate cancers, the commonly occurring PTEN mutation is associated with tumor progression toward aggressive stage, increased vascular density and angiogenesis.[64]

During hypoxia, tumor suppressor p53 overexpression may be associated with HIF1A-dependent pathway to initiate apoptosis. Moreover, p53-independent pathway may also induce apoptosis through the Bcl-2 pathway.[52] However, overexpression of HIF1A is cancer- and individual-specific, and depends on the accompanying genetic alternations and levels of pro- and anti-apoptotic factors present. One study on epithelial ovarian cancer shows HIF1A and nonfunctional tumor suppressor p53 is correlated with low levels of tumor cell apoptosis and poor prognosis.[65] Further, early-stage esophageal cancer patients with demonstrated overexpression of HIF1 and absence of BCL2 expression also failed photodynamic therapy.[66]

While research efforts to develop therapeutic drugs to target hypoxia-associated tumor cells have been ongoing for many years, there has not yet been any breakthrough that has shown selectivity and effectiveness at targeting HIF1A pathways to decrease tumor progression and angiogenesis.[67] Successful therapeutic approaches in the future may also be highly case-specific to particular cancers and individuals, and seem unlikely to be widely applicable due to the genetically heterogenous nature of the many cancer types and subtypes.

Interactions

HIF1A has been shown to interact with:

• ARNTL,[68]
• ARNT,[6][69]
• CREBB,[70][71][72]
• EP300,[38][73]
• HIF1AN,[74]
• Mdm2,[75][76]
• NR4A,[77]
• P53,[75][76][78][79]
• PSMA7,[80]
• STAT3,[81]
• UBC,[72][77][82]
• VH[72][74][77][81][82][83][84][85][37][86] and
• VHL.[87]
• GR (NR3C1).[88][89]

See also

• Hypoxia inducible factors

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Further reading

• Semenza GL (August 2000). "HIF-1 and human disease: one highly involved factor". Genes & Development. 14 (16): 1983–91. PMID 10950862.
• Semenza G (September 2002). "Signal transduction to hypoxia-inducible factor 1". Biochemical Pharmacology. 64 (5–6): 993–8. doi:10.1016/S0006-2952(02)01168-1. PMID 12213597.
• Arbeit JM (2002). "Quiescent hypervascularity mediated by gain of HIF-1 alpha function". Cold Spring Harbor Symposia on Quantitative Biology. 67: 133–42. doi:10.1101/sqb.2002.67.133. PMID 12858534.
• Sitkovsky M, Lukashev D (September 2005). "Regulation of immune cells by local-tissue oxygen tension: HIF1 alpha and adenosine receptors". Nature Reviews. Immunology. 5 (9): 712–21. doi:10.1038/nri1685. PMID 16110315.
• Mobasheri A, Richardson S, Mobasheri R, Shakibaei M, Hoyland JA (October 2005). "Hypoxia inducible factor-1 and facilitative glucose transporters GLUT1 and GLUT3: putative molecular components of the oxygen and glucose sensing apparatus in articular chondrocytes". Histology and Histopathology. 20 (4): 1327–38. doi:10.14670/HH-20.1327. PMID 16136514.
• Schipani E (2006). "Hypoxia and HIF-1 alpha in chondrogenesis". Seminars in Cell & Developmental Biology. 16 (4–5): 539–46. doi:10.1016/j.semcdb.2005.03.003. PMID 16144691.
• Haase VH (August 2006). "Hypoxia-inducible factors in the kidney". American Journal of Physiology. Renal Physiology. 291 (2): F271–81. doi:10.1152/ajprenal.00071.2006. PMC 4232221. PMID 16554418.
• Liang D, Kong X, Sang N (November 2006). "Effects of histone deacetylase inhibitors on HIF-1". Cell Cycle. 5 (21): 2430–5. doi:10.4161/cc.5.21.3409. PMC 4505804. PMID 17102633.

External links

• Overview of all the structural information available in the PDB for UniProt: Q16665 (Human Hypoxia-inducible factor 1-alpha) at the PDBe-KB.
• Overview of all the structural information available in the PDB for UniProt: Q61221 (Mouse Hypoxia-inducible factor 1-alpha) at the PDBe-KB.