EGR2

Early growth response protein 2 is a protein that in humans is encoded by the EGR2 gene. EGR2 (also termed Krox20) is a transcription regulatory factor, containing three zinc finger DNA-binding sites, and is highly expressed in a population of migrating neural crest cells.[5][6][7] It is later expressed in the neural crest derived cells of the cranial ganglion. The protein encoded by Krox20 contains two cys2his2-type zinc fingers. Krox20 gene expression is restricted to the early hindbrain development.[6][8] It is evolutionarily conserved in vertebrates, humans, mice, chicks, and zebra fish.[9] In addition, the amino acid sequence and most aspects of the embryonic gene pattern is conserved among vertebrates, further implicating its role in hindbrain development.[7][10][11][12] When the Krox20 is deleted in mice, the protein coding ability of the Krox20 gene (including the DNA-binding domain of the zinc finger) is diminished. These mice are unable to survive after birth and exhibit major hindbrain defects.[6][8] These defects include but are not limited to defects in formation of cranial sensory ganglia, partial fusion of the trigeminal nerve (V) with the facial (VII) and auditory (VII) nerves, the proximal nerve roots coming off of these ganglia were disorganized and intertwined among one another as they entered the brainstem, and there was fusion of the glossopharyngeal (IX) nerve complex.[13][14][15]

EGR2
Identifiers
Aliases	EGR2, AT591, CMT1D, CMT4E, KROX20, early growth response 2, CHN1
External IDs	OMIM: 129010 MGI: 95296 HomoloGene: 20123 GeneCards: EGR2
Gene location (Human) Chr. Chromosome 10 (human)[1] Band 10q21.3 Start 62,811,996 bp[1] End 62,919,900 bp[1]
Gene location (Mouse) Chr. Chromosome 10 (mouse)[2] Band 10 B5.1|10 34.96 cM Start 67,535,475 bp[2] End 67,542,188 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • DNA binding • GO:0001131, GO:0001151, GO:0001130, GO:0001204 DNA-binding transcription factor activity • HMG box domain binding • GO:0001077, GO:0001212, GO:0001213, GO:0001211, GO:0001205 DNA-binding transcription activator activity, RNA polymerase II-specific • chromatin binding • metal ion binding • RNA polymerase II activating transcription factor binding • GO:0000980 RNA polymerase II cis-regulatory region sequence-specific DNA binding • GO:0001948 protein binding • nucleic acid binding • ubiquitin protein ligase binding • transferase activity • GO:0001200, GO:0001133, GO:0001201 DNA-binding transcription factor activity, RNA polymerase II-specific • SUMO ligase activity Cellular component • cytoplasm • cell nucleus • nucleoplasm • intracellular membrane-bounded organelle Biological process • cellular response to organic substance • rhythmic behavior • peripheral nervous system development • regulation of transcription, DNA-templated • rhombomere 3 development • brain segmentation • transcription from RNA polymerase II promoter • protein sumoylation • rhombomere 3 formation • transcription, DNA-templated • response to insulin • positive regulation of transcription, DNA-templated • brain development • rhombomere 5 formation • protein export from nucleus • learning or memory • skeletal muscle cell differentiation • Schwann cell differentiation • regulation of neuronal synaptic plasticity • myelination • facial nerve structural organization • regulation of ossification • motor neuron axon guidance • fat cell differentiation • positive regulation of transcription from RNA polymerase II promoter • regulation of transcription from RNA polymerase II promoter Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	1959	13654
Ensembl	ENSG00000122877	ENSMUSG00000037868
UniProt	P11161	P08152
RefSeq (mRNA)	NM_000399 NM_001136177 NM_001136178 NM_001136179 NM_001321037	NM_010118 NM_001347458
RefSeq (protein)	NP_000390 NP_001129649 NP_001129650 NP_001129651 NP_001307966	NP_001334387 NP_034248 NP_001360912 NP_001360914 NP_001360915 NP_001360916
Location (UCSC)	Chr 10: 62.81 – 62.92 Mb	Chr 10: 67.54 – 67.54 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Function

The early growth response protein 2 is a transcription factor with three tandem C2H2-type zinc fingers. Mutations in this gene are associated with the autosomal dominant Charcot-Marie-Tooth disease, type 1D,[16] Dejerine–Sottas disease,[17] and Congenital Hypomyelinating Neuropathy.[18] Two studies have linked EGR2 expression to proliferation of osteoprogenitors [19] and cell lines derived from Ewing sarcoma, which is a highly aggressive bone-associated cancer.[20]

New research suggests that Krox20 - or the lack of it - is the reason for male baldness.[21]

References

1. GRCh38: Ensembl release 89: ENSG00000122877 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000037868 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Chavrier P, Janssen-Timmen U, Mattéi MG, Zerial M, Bravo R, Charnay P (February 1989). "Structure, chromosome location, and expression of the mouse zinc finger gene Krox-20: multiple gene products and coregulation with the proto-oncogene c-fos". Molecular and Cellular Biology. 9 (2): 787–97. doi:10.1128/mcb.9.2.787. PMC 362656. PMID 2496302.
6. Swiatek PJ, Gridley T (November 1993). "Perinatal lethality and defects in hindbrain development in mice homozygous for a targeted mutation of the zinc finger gene Krox20". Genes & Development. 7 (11): 2071–84. doi:10.1101/gad.7.11.2071. PMID 8224839.
7. Wilkinson DG, Bhatt S, Chavrier P, Bravo R, Charnay P (February 1989). "Segment-specific expression of a zinc-finger gene in the developing nervous system of the mouse". Nature. 337 (6206): 461–4. doi:10.1038/337461a0. PMID 2915691.
8. Bradley LC, Snape A, Bhatt S, Wilkinson DG (January 1993). "The structure and expression of the Xenopus Krox-20 gene: conserved and divergent patterns of expression in rhombomeres and neural crest". Mechanisms of Development. 40 (1–2): 73–84. doi:10.1016/0925-4773(93)90089-g. PMID 8443108.
9. Bhat RV, Worley PF, Cole AJ, Baraban JM (April 1992). "Activation of the zinc finger encoding gene krox-20 in adult rat brain: comparison with zif268". Brain Research. Molecular Brain Research. 13 (3): 263–6. doi:10.1016/0169-328x(92)90034-9. PMID 1317498.
10. Wilkinson DG, Bhatt S, Cook M, Boncinelli E, Krumlauf R (October 1989). "Segmental expression of Hox-2 homoeobox-containing genes in the developing mouse hindbrain". Nature. 341 (6241): 405–9. doi:10.1038/341405a0. PMID 2571936.
11. Hunt P, Gulisano M, Cook M, Sham MH, Faiella A, Wilkinson D, Boncinelli E, Krumlauf R (October 1991). "A distinct Hox code for the branchial region of the vertebrate head". Nature. 353 (6347): 861–4. doi:10.1038/353861a0. PMID 1682814.
12. Oxtoby E, Jowett T (March 1993). "Cloning of the zebrafish krox-20 gene (krx-20) and its expression during hindbrain development". Nucleic Acids Research. 21 (5): 1087–95. doi:10.1093/nar/21.5.1087. PMC 309267. PMID 8464695.
13. Frohman MA, Boyle M, Martin GR (October 1990). "Isolation of the mouse Hox-2.9 gene; analysis of embryonic expression suggests that positional information along the anterior-posterior axis is specified by mesoderm". Development. 110 (2): 589–607. PMID 1983472.
14. Murphy P, Davidson DR, Hill RE (September 1989). "Segment-specific expression of a homoeobox-containing gene in the mouse hindbrain". Nature. 341 (6238): 156–9. doi:10.1038/341156a0. PMID 2571087.
15. Nieto MA, Bradley LC, Wilkinson DG (1991). "Conserved segmental expression of Krox-20 in the vertebrate hindbrain and its relationship to lineage restriction". Development. Suppl 2: 59–62. PMID 1688180.
16. "Entrez Gene: EGR2 early growth response 2 (Krox-20 homolog, Drosophila)".
17. Boerkoel CF, Takashima H, Bacino CA, Daentl D, Lupski JR (July 2001). "EGR2 mutation R359W causes a spectrum of Dejerine-Sottas neuropathy". Neurogenetics. 3 (3): 153–7. doi:10.1007/s100480100107. PMID 11523566.
18. Warner LE, Mancias P, Butler IJ, McDonald CM, Keppen L, Koob KG, Lupski JR (April 1998). "Mutations in the early growth response 2 (EGR2) gene are associated with hereditary myelinopathies". Nature Genetics. 18 (4): 382–4. doi:10.1038/ng0498-382. PMID 9537424.
19. Chandra A, Lan S, Zhu J, Siclari VA, Qin L (July 2013). "Epidermal growth factor receptor (EGFR) signaling promotes proliferation and survival in osteoprogenitors by increasing early growth response 2 (EGR2) expression". The Journal of Biological Chemistry. 288 (28): 20488–98. doi:10.1074/jbc.M112.447250. PMC 3711314. PMID 23720781.
20. Grünewald TG, Bernard V, Gilardi-Hebenstreit P, Raynal V, Surdez D, Aynaud MM, et al. (September 2015). "Chimeric EWSR1-FLI1 regulates the Ewing sarcoma susceptibility gene EGR2 via a GGAA microsatellite". Nature Genetics. 47 (9): 1073–8. doi:10.1038/ng.3363. PMC 4591073. PMID 26214589.
21. Le, Lu. "Scientists find skin cells at the root of balding, gray hair". UT Southwestern Medical Center. Retrieved 9 May 2017.

Further reading

• Rangnekar VM, Aplin AC, Sukhatme VP (May 1990). "The serum and TPA responsive promoter and intron-exon structure of EGR2, a human early growth response gene encoding a zinc finger protein". Nucleic Acids Research. 18 (9): 2749–57. doi:10.1093/nar/18.9.2749. PMC 330760. PMID 2111009.
• Chavrier P, Janssen-Timmen U, Mattéi MG, Zerial M, Bravo R, Charnay P (February 1989). "Structure, chromosome location, and expression of the mouse zinc finger gene Krox-20: multiple gene products and coregulation with the proto-oncogene c-fos". Molecular and Cellular Biology. 9 (2): 787–97. doi:10.1128/mcb.9.2.787. PMC 362656. PMID 2496302.
• Joseph LJ, Le Beau MM, Jamieson GA, Acharya S, Shows TB, Rowley JD, Sukhatme VP (October 1988). "Molecular cloning, sequencing, and mapping of EGR2, a human early growth response gene encoding a protein with "zinc-binding finger" structure". Proceedings of the National Academy of Sciences of the United States of America. 85 (19): 7164–8. doi:10.1073/pnas.85.19.7164. PMC 282144. PMID 3140236.
• Topilko P, Schneider-Maunoury S, Levi G, Baron-Van Evercooren A, Chennoufi AB, Seitanidou T, Babinet C, Charnay P (October 1994). "Krox-20 controls myelination in the peripheral nervous system". Nature. 371 (6500): 796–9. doi:10.1038/371796a0. PMID 7935840.
• Sham MH, Vesque C, Nonchev S, Marshall H, Frain M, Gupta RD, Whiting J, Wilkinson D, Charnay P, Krumlauf R (January 1993). "The zinc finger gene Krox20 regulates HoxB2 (Hox2.8) during hindbrain segmentation". Cell. 72 (2): 183–96. doi:10.1016/0092-8674(93)90659-E. PMID 8093858.
• Levi G, Topilko P, Schneider-Maunoury S, Lasagna M, Mantero S, Pesce B, Ghersi G, Cancedda R, Charnay P (June 1996). "Role of Krox-20 in endochondral bone formation". Annals of the New York Academy of Sciences. 785: 288–91. doi:10.1111/j.1749-6632.1996.tb56286.x. PMID 8702157.
• Warner LE, Mancias P, Butler IJ, McDonald CM, Keppen L, Koob KG, Lupski JR (April 1998). "Mutations in the early growth response 2 (EGR2) gene are associated with hereditary myelinopathies". Nature Genetics. 18 (4): 382–4. doi:10.1038/ng0498-382. PMID 9537424.
• Warner LE, Svaren J, Milbrandt J, Lupski JR (July 1999). "Functional consequences of mutations in the early growth response 2 gene (EGR2) correlate with severity of human myelinopathies". Human Molecular Genetics. 8 (7): 1245–51. doi:10.1093/hmg/8.7.1245. PMID 10369870.
• Timmerman V, De Jonghe P, Ceuterick C, De Vriendt E, Löfgren A, Nelis E, Warner LE, Lupski JR, Martin JJ, Van Broeckhoven C (June 1999). "Novel missense mutation in the early growth response 2 gene associated with Dejerine-Sottas syndrome phenotype". Neurology. 52 (9): 1827–32. doi:10.1212/wnl.52.9.1827. PMID 10371530.
• Bellone E, Di Maria E, Soriani S, Varese A, Doria LL, Ajmar F, Mandich P (October 1999). "A novel mutation (D305V) in the early growth response 2 gene is associated with severe Charcot-Marie-Tooth type 1 disease". Human Mutation. 14 (4): 353–4. doi:10.1002/(SICI)1098-1004(199910)14:4<353::AID-HUMU17>3.0.CO;2-4. PMID 10502832.
• Pareyson D, Taroni F, Botti S, Morbin M, Baratta S, Lauria G, Ciano C, Sghirlanzoni A (April 2000). "Cranial nerve involvement in CMT disease type 1 due to early growth response 2 gene mutation". Neurology. 54 (8): 1696–8. doi:10.1212/wnl.54.8.1696. hdl:2434/531868. PMID 10762521.
• Gambardella L, Schneider-Maunoury S, Voiculescu O, Charnay P, Barrandon Y (September 2000). "Pattern of expression of the transcription factor Krox-20 in mouse hair follicle". Mechanisms of Development. 96 (2): 215–8. doi:10.1016/S0925-4773(00)00398-1. PMID 10960786.
• Yoshihara T, Kanda F, Yamamoto M, Ishihara H, Misu K, Hattori N, Chihara K, Sobue G (March 2001). "A novel missense mutation in the early growth response 2 gene associated with late-onset Charcot--Marie--Tooth disease type 1". Journal of the Neurological Sciences. 184 (2): 149–53. doi:10.1016/S0022-510X(00)00504-9. PMID 11239949.
• Boerkoel CF, Takashima H, Bacino CA, Daentl D, Lupski JR (July 2001). "EGR2 mutation R359W causes a spectrum of Dejerine-Sottas neuropathy". Neurogenetics. 3 (3): 153–7. doi:10.1007/s100480100107. PMID 11523566.
• Yang Y, Dong B, Mittelstadt PR, Xiao H, Ashwell JD (May 2002). "HIV Tat binds Egr proteins and enhances Egr-dependent transactivation of the Fas ligand promoter". The Journal of Biological Chemistry. 277 (22): 19482–7. doi:10.1074/jbc.M201687200. PMID 11909874.
• Vandenberghe N, Upadhyaya M, Gatignol A, Boutrand L, Boucherat M, Chazot G, Vandenberghe A, Latour P (December 2002). "Frequency of mutations in the early growth response 2 gene associated with peripheral demyelinating neuropathies". Journal of Medical Genetics. 39 (12): 81e–81. doi:10.1136/jmg.39.12.e81. PMC 1757229. PMID 12471219.
• Musso M, Balestra P, Taroni F, Bellone E, Mandich P (February 2003). "Different consequences of EGR2 mutants on the transactivation of human Cx32 promoter". Neurobiology of Disease. 12 (1): 89–95. doi:10.1016/S0969-9961(02)00018-9. PMID 12609493.
• Unoki M, Nakamura Y (April 2003). "EGR2 induces apoptosis in various cancer cell lines by direct transactivation of BNIP3L and BAK". Oncogene. 22 (14): 2172–85. doi:10.1038/sj.onc.1206222. PMID 12687019.
• Numakura C, Shirahata E, Yamashita S, Kanai M, Kijima K, Matsuki T, Hayasaka K (June 2003). "Screening of the early growth response 2 gene in Japanese patients with Charcot-Marie-Tooth disease type 1". Journal of the Neurological Sciences. 210 (1–2): 61–4. doi:10.1016/S0022-510X(03)00028-5. PMID 12736090.

External links

• GeneReviews/NCBI/NIH/UW entry on Charcot-Marie-Tooth Neuropathy Type 1
• GeneReviews/NCBI/NIH/UW entry on Charcot-Marie-Tooth Neuropathy Type 4
• EGR2+protein,+human at the US National Library of Medicine Medical Subject Headings (MeSH)

This article incorporates text from the United States National Library of Medicine, which is in the public domain.