5-HT_1B receptor

5-hydroxytryptamine receptor 1B also known as the 5-HT_1B receptor is a protein that in humans is encoded by the HTR1B gene.[5][6] The 5-HT_1B receptor is a 5-HT receptor subtype.[7]

HTR1B
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 4IAQ, 4IAR
Identifiers
Aliases	HTR1B, 5-HT1B, 5-HT1DB, HTR1D2, HTR1DB, S12, 5-HT-1B, 5-HT-1D-beta, 5-hydroxytryptamine receptor 1B
External IDs	OMIM: 182131 MGI: 96274 HomoloGene: 669 GeneCards: HTR1B
Gene location (Human) Chr. Chromosome 6 (human)[1] Band 6q14.1 Start 77,461,753 bp[1] End 77,463,773 bp[1]
Gene location (Mouse) Chr. Chromosome 9 (mouse)[2] Band 9 E1|9 44.61 cM Start 81,628,291 bp[2] End 81,633,828 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • G-protein coupled receptor activity • signal transducer activity • G-protein coupled serotonin receptor activity • GO:0001948 protein binding • drug binding • neurotransmitter receptor activity • serotonin binding • voltage-gated calcium channel activity involved in regulation of presynaptic cytosolic calcium levels Cellular component • cytoplasm • integral component of membrane • membrane • integral component of plasma membrane • cell membrane • calyx of Held • integral component of presynaptic membrane • serotonergic synapse • dendrite Biological process • chemical synaptic transmission • G-protein coupled receptor internalization • regulation of dopamine secretion • regulation of behavior • response to mineralocorticoid • vasoconstriction • adenylate cyclase-inhibiting serotonin receptor signaling pathway • G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger • cellular response to alkaloid • negative regulation of serotonin secretion • cellular response to temperature stimulus • cellular response to drug • negative regulation of synaptic transmission, GABAergic • protein kinase C-activating G-protein coupled receptor signaling pathway • drinking behavior • bone remodeling • feeding behavior • response to ethanol • negative regulation of synaptic transmission, glutamatergic • response to drug • signal transduction • response to cocaine • positive regulation of vascular smooth muscle cell proliferation • G-protein coupled receptor signaling pathway • adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway • animal behavior • presynaptic modulation of chemical synaptic transmission • regulation of synaptic vesicle exocytosis • regulation of presynaptic cytosolic calcium ion concentration Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	3351	15551
Ensembl	ENSG00000135312	ENSMUSG00000049511
UniProt	P28222	P28334
RefSeq (mRNA)	NM_000863	NM_010482
RefSeq (protein)	NP_000854	NP_034612
Location (UCSC)	Chr 6: 77.46 – 77.46 Mb	Chr 9: 81.63 – 81.63 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Tissue distribution and function

5-HT_1B receptors are widely distributed throughout the central nervous system with the highest concentrations found in the frontal cortex, basal ganglia, striatum, and the hippocampus.[8] The function of the 5-HT_1B receptor differs depending upon its location. In the frontal cortex, it is believed to act as a postsynaptic receptor inhibiting the release of dopamine. In the basal ganglia and the striatum, evidence suggests 5-HT signaling acts on an autoreceptor, inhibiting the release of serotonin[9] and decreasing glutamatergic transmission by reducing miniature excitatory postsynaptic potential (mEPSP) frequency,[10] respectively. In the hippocampus, a recent study has demonstrated that activation of postsynaptic 5-HT_1B heteroreceptors produces a facilitation in excitatory synaptic transmission which is altered in depression.[11] When the expression of 5-HT_1B in human cortex was traced throughout life, significant changes during adolescence were observed, in a way that is strongly correlated with the expression of 5-HT_1E.[12]

Outside the brain, 5-HT_1B receptor activation also has vascular effects, such as pulmonary vasoconstriction. Furthermore, blocking 5-HT_1B receptor signalling increases the number of osteoblasts, bone mass, and the bone formation rate.[13]

Knockout mice lacking the 5-HT_1B gene have shown an increase in aggression and a higher preference for alcohol.[14] Under basal conditions, knockout mice present with a "normal" phenotype and exhibit a sucrose preference (lack of sucrose preference is considered a measure of anhedonia). However, after undergoing chronic unpredictable stress treatment to induce a "depression-like" phenotype these animals do not benefit from administration of selective serotonin reuptake inhibitor (SSRIs).[11]

Ligands

Agonists

• Ergotamine (vasoconstrictor in migraine)
• Oxymetazoline
• Sumatriptan (vasoconstrictor in migraine)
• Zolmitriptan
• 5-Carboxamidotryptamine
• CGS-12066A
• CP-93,129 (peripherally acting)
• CP-94,253
• CP-122,288 (mixed 5-HT_1B/1D agonist)
• CP-135,807 (mixed 5-HT_1B/1D agonist)
• RU-24969 (mixed 5-HT_1A/1B agonist)

Partial agonists

• Ziprasidone (antipsychotic)
• Asenapine (antipsychotic)
• Vortioxetine (antidepressant)

Antagonists and inverse agonists

• Methiothepin (antipsychotic)
• Yohimbine (aphrodisiac)
• Metergoline
• Aripiprazole
• Isamoltane
• AR-A000002[15]
• SB-216,641
• SB-224,289 (inverse agonist)[16]
• SB-236,057 (inverse agonist)[17]

Undetermined Action

• Dextromethorphan (Cough Suppressant)[18]

Genetics

In humans the protein is coded by the gene HTR1B.

A genetic variant in the promoter region, A-161T, has been examined with respect to personality traits and showed no major effect.[19]

See also

• 5-HT₁ receptor
• 5-HT receptor

References

1. GRCh38: Ensembl release 89: ENSG00000135312 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000049511 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Jin H, Oksenberg D, Ashkenazi A, Peroutka SJ, Duncan AM, Rozmahel R, Yang Y, Mengod G, Palacios JM, O'Dowd BF (Mar 1992). "Characterization of the human 5-hydroxytryptamine1B receptor". The Journal of Biological Chemistry. 267 (9): 5735–8. PMID 1348246.
6. Sanders AR, Cao Q, Taylor J, Levin TE, Badner JA, Cravchik A, Comeron JM, Naruya S, Del Rosario A, Salvi DA, Walczyk KA, Mowry BJ, Levinson DF, Crowe RR, Silverman JM, Gejman PV (Feb 2001). "Genetic diversity of the human serotonin receptor 1B (HTR1B) gene". Genomics. 72 (1): 1–14. doi:10.1006/geno.2000.6411. PMID 11247661.
7. "Entrez Gene: HTR1B 5-hydroxytryptamine (serotonin) receptor 1B".
8. "5-hydroxytryptamine (serotonin) receptor 1B, G protein-coupled". Retrieved 23 Feb 2013.
9. Pytliak M, Vargová V, Mechírová V, Felšöci M (2011). "Serotonin receptors - from molecular biology to clinical applications". Physiological Research. 60 (1): 15–25. doi:10.33549/physiolres.931903. PMID 20945968.
10. Huang CC, Yeh CM, Wu MY, Hsu KS (Jun 2013). "A single in vivo cocaine administration impairs 5-HT(1B) receptor-induced long-term depression in the nucleus accumbens". Journal of Neurochemistry. 125 (6): 809–21. doi:10.1111/jnc.12227. PMID 23452061.
11. Cai X, Kallarackal AJ, Kvarta MD, Goluskin S, Gaylor K, Bailey AM, Lee HK, Huganir RL, Thompson SM (Apr 2013). "Local potentiation of excitatory synapses by serotonin and its alteration in rodent models of depression". Nature Neuroscience. 16 (4): 464–72. doi:10.1038/nn.3355. PMC 3609911. PMID 23502536.
12. Shoval G, Bar-Shira O, Zalsman G, John Mann J, Chechik G (Jul 2014). "Transitions in the transcriptome of the serotonergic and dopaminergic systems in the human brain during adolescence". European Neuropsychopharmacology. 24 (7): 1123–32. doi:10.1016/j.euroneuro.2014.02.009. PMID 24721318.
13. Yadav VK, Ryu JH, Suda N, Tanaka KF, Gingrich JA, Schütz G, Glorieux FH, Chiang CY, Zajac JD, Insogna KL, Mann JJ, Hen R, Ducy P, Karsenty G (Nov 2008). "Lrp5 controls bone formation by inhibiting serotonin synthesis in the duodenum". Cell. 135 (5): 825–37. doi:10.1016/j.cell.2008.09.059. PMC 2614332. PMID 19041748.
14. Hoyer D, Hannon JP, Martin GR (Apr 2002). "Molecular, pharmacological and functional diversity of 5-HT receptors". Pharmacology Biochemistry and Behavior. 71 (4): 533–54. doi:10.1016/S0091-3057(01)00746-8. PMID 11888546.
15. Hudzik TJ, Yanek M, Porrey T, Evenden J, Paronis C, Mastrangelo M, Ryan C, Ross S, Stenfors C (Mar 2003). "Behavioral pharmacology of AR-A000002, a novel, selective 5-hydroxytryptamine(1B) antagonist". The Journal of Pharmacology and Experimental Therapeutics. 304 (3): 1072–84. doi:10.1124/jpet.102.045468. PMID 12604684.
16. Selkirk JV, Scott C, Ho M, Burton MJ, Watson J, Gaster LM, Collin L, Jones BJ, Middlemiss DN, Price GW (Sep 1998). "SB-224289--a novel selective (human) 5-HT1B receptor antagonist with negative intrinsic activity". British Journal of Pharmacology. 125 (1): 202–8. doi:10.1038/sj.bjp.0702059. PMC 1565605. PMID 9776361.
17. Roberts C, Watson J, Price GW, Middlemiss DN (2001). "SB-236057-A: a selective 5-HT1B receptor inverse agonist". CNS Drug Reviews. 7 (4): 433–44. doi:10.1111/j.1527-3458.2001.tb00209.x. PMC 6741665. PMID 11830759.
18. Nguyen L, Thomas KL, Lucke-Wold BP, Cavendish JZ, Crowe MS, Matsumoto RR (2016). "Dextromethorphan: An update on its utility for neurological and neuropsychiatric disorders". Pharmacol. Ther. 159: 1–22. doi:10.1016/j.pharmthera.2016.01.016. PMID 26826604.
19. Tsai SJ, Wang YC, Chen JY, Hong CJ (2003). "Allelic variants of the tryptophan hydroxylase (A218C) and serotonin 1B receptor (A-161T) and personality traits". Neuropsychobiology. 48 (2): 68–71. doi:10.1159/000072879. PMID 14504413.

Further reading

• Olivier B, van Oorschot R (Dec 2005). "5-HT1B receptors and aggression: a review". European Journal of Pharmacology. 526 (1–3): 207–17. doi:10.1016/j.ejphar.2005.09.066. PMID 16310769.
• Hamblin MW, Metcalf MA, McGuffin RW, Karpells S (Apr 1992). "Molecular cloning and functional characterization of a human 5-HT1B serotonin receptor: a homologue of the rat 5-HT1B receptor with 5-HT1D-like pharmacological specificity". Biochemical and Biophysical Research Communications. 184 (2): 752–9. doi:10.1016/0006-291X(92)90654-4. PMID 1315531.
• Veldman SA, Bienkowski MJ (Sep 1992). "Cloning and pharmacological characterization of a novel human 5-hydroxytryptamine1D receptor subtype". Molecular Pharmacology. 42 (3): 439–44. PMID 1328844.
• Demchyshyn L, Sunahara RK, Miller K, Teitler M, Hoffman BJ, Kennedy JL, Seeman P, Van Tol HH, Niznik HB (Jun 1992). "A human serotonin 1D receptor variant (5HT1D beta) encoded by an intronless gene on chromosome 6". Proceedings of the National Academy of Sciences of the United States of America. 89 (12): 5522–6. doi:10.1073/pnas.89.12.5522. PMC 49324. PMID 1351684.
• Levy FO, Gudermann T, Perez-Reyes E, Birnbaumer M, Kaumann AJ, Birnbaumer L (Apr 1992). "Molecular cloning of a human serotonin receptor (S12) with a pharmacological profile resembling that of the 5-HT1D subtype". The Journal of Biological Chemistry. 267 (11): 7553–62. PMID 1559993.
• Weinshank RL, Zgombick JM, Macchi MJ, Branchek TA, Hartig PR (Apr 1992). "Human serotonin 1D receptor is encoded by a subfamily of two distinct genes: 5-HT1D alpha and 5-HT1D beta". Proceedings of the National Academy of Sciences of the United States of America. 89 (8): 3630–4. doi:10.1073/pnas.89.8.3630. PMC 48922. PMID 1565658.
• Mochizuki D, Yuyama Y, Tsujita R, Komaki H, Sagai H (Jun 1992). "Cloning and expression of the human 5-HT1B-type receptor gene". Biochemical and Biophysical Research Communications. 185 (2): 517–23. doi:10.1016/0006-291X(92)91655-A. PMID 1610347.
• Nöthen MM, Erdmann J, Shimron-Abarbanell D, Propping P (Dec 1994). "Identification of genetic variation in the human serotonin 1D beta receptor gene". Biochemical and Biophysical Research Communications. 205 (2): 1194–200. doi:10.1006/bbrc.1994.2792. PMID 7802650.
• Ng GY, George SR, Zastawny RL, Caron M, Bouvier M, Dennis M, O'Dowd BF (Nov 1993). "Human serotonin1B receptor expression in Sf9 cells: phosphorylation, palmitoylation, and adenylyl cyclase inhibition". Biochemistry. 32 (43): 11727–33. doi:10.1021/bi00094a032. PMID 8218242.
• Bouchelet I, Cohen Z, Case B, Séguéla P, Hamel E (Aug 1996). "Differential expression of sumatriptan-sensitive 5-hydroxytryptamine receptors in human trigeminal ganglia and cerebral blood vessels". Molecular Pharmacology. 50 (2): 219–23. PMID 8700126.
• Varnäs K, Hall H, Bonaventure P, Sedvall G (Oct 2001). "Autoradiographic mapping of 5-HT(1B) and 5-HT(1D) receptors in the post mortem human brain using [(3)H]GR 125743". Brain Research. 915 (1): 47–57. doi:10.1016/S0006-8993(01)02823-2. PMID 11578619.
• Salim K, Fenton T, Bacha J, Urien-Rodriguez H, Bonnert T, Skynner HA, Watts E, Kerby J, Heald A, Beer M, McAllister G, Guest PC (May 2002). "Oligomerization of G-protein-coupled receptors shown by selective co-immunoprecipitation". The Journal of Biological Chemistry. 277 (18): 15482–5. doi:10.1074/jbc.M201539200. PMID 11854302.
• Hasegawa Y, Higuchi S, Matsushita S, Miyaoka H (Apr 2002). "Association of a polymorphism of the serotonin 1B receptor gene and alcohol dependence with inactive aldehyde dehydrogenase-2". Journal of Neural Transmission. 109 (4): 513–21. doi:10.1007/s007020200042. PMID 11956970.
• Hawi Z, Dring M, Kirley A, Foley D, Kent L, Craddock N, Asherson P, Curran S, Gould A, Richards S, Lawson D, Pay H, Turic D, Langley K, Owen M, O'Donovan M, Thapar A, Fitzgerald M, Gill M (2003). "Serotonergic system and attention deficit hyperactivity disorder (ADHD): a potential susceptibility locus at the 5-HT(1B) receptor gene in 273 nuclear families from a multi-centre sample". Molecular Psychiatry. 7 (7): 718–25. doi:10.1038/sj.mp.4001048. PMID 12192616.
• Huang YY, Oquendo MA, Friedman JM, Greenhill LL, Brodsky B, Malone KM, Khait V, Mann JJ (Jan 2003). "Substance abuse disorder and major depression are associated with the human 5-HT1B receptor gene (HTR1B) G861C polymorphism". Neuropsychopharmacology. 28 (1): 163–9. doi:10.1038/sj.npp.1300000. PMID 12496953.
• Quist JF, Barr CL, Schachar R, Roberts W, Malone M, Tannock R, Basile VS, Beitchman J, Kennedy JL (Jan 2003). "The serotonin 5-HT1B receptor gene and attention deficit hyperactivity disorder". Molecular Psychiatry. 8 (1): 98–102. doi:10.1038/sj.mp.4001244. PMID 12556913.
• Sinha R, Cloninger CR, Parsian A (Aug 2003). "Linkage disequilibrium and haplotype analysis between serotonin receptor 1B gene variations and subtypes of alcoholism". American Journal of Medical Genetics Part B. 121B (1): 83–8. doi:10.1002/ajmg.b.20064. PMID 12898580.

External links

• "5-HT_1B". IUPHAR Database of Receptors and Ion Channels. International Union of Basic and Clinical Pharmacology.
• Human HTR1B genome location and HTR1B gene details page in the UCSC Genome Browser.
• Overview of all the structural information available in the PDB for UniProt: P28222 (5-hydroxytryptamine receptor 1B) at the PDBe-KB.

This article incorporates text from the United States National Library of Medicine, which is in the public domain.