GPR113

GPR113 is a gene that encodes the Probable G-protein coupled receptor 113 protein.[5][6]

ADGRF3
Identifiers
Aliases	ADGRF3, PGR23, hGPCR37, GPR113, adhesion G protein-coupled receptor F3
External IDs	MGI: 2685887 HomoloGene: 17826 GeneCards: ADGRF3
Gene location (Human) Chr. Chromosome 2 (human)[1] Band 2p23.3 Start 26,308,173 bp[1] End 26,346,817 bp[1]
Gene location (Mouse) Chr. Chromosome 5 (mouse)[2] Band 5|5 B1 Start 30,193,431 bp[2] End 30,205,722 bp[2]
Gene ontology Molecular function • G-protein coupled receptor activity • transmembrane signaling receptor activity • signal transducer activity Cellular component • integral component of membrane • membrane Biological process • G-protein coupled receptor signaling pathway • cell surface receptor signaling pathway • signal transduction Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	165082	381628
Ensembl	ENSG00000173567	ENSMUSG00000067642
UniProt	Q8IZF5	Q58Y75
RefSeq (mRNA)	NM_001145168 NM_001145169 NM_153835 NM_001321971 NM_001321975	NM_001014394
RefSeq (protein)	NP_001138640 NP_001138641 NP_001308900 NP_001308904 NP_722577	NP_001014416
Location (UCSC)	Chr 2: 26.31 – 26.35 Mb	Chr 5: 30.19 – 30.21 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Gene

The Homo sapiens GPR113 gene is located on chromosome 2 (2p23.3). This gene spans the length of a 38.65kb region from base 26531041 to 26569685 on the negative strand.[7] The GPR113 gene has two neighbors on either side on the negative strand: OTOF otoferlin preceding and HADHA hydroxyacyl-CoA following. Directly opposite the GPR113 on the positive strand is the EPT1 gene. The GPR113 gene is also known by the aliases PGR23 and HGPCR37.[8]

Homology & Evolution

The GPR113 has 5 human paralogs GPR110, GPR115, GPR128, GPR111, and GPR116.[8] GPR113 is well conserved in mammals from primates to semi-aquatic species, as well as some amphibians. These include the Common Chimpanzee, the African Bush Elephant, the Platypus, and the Western Clawed Frog.[9] Homologous domains that are well conserved throughout orthologs center in the 7 transmembrane receptor (Secretin family) region highlighted in purple in the figure.[10]

Protein

Annotated primary structure of the GPR113 Protein

The protein product of GPR113 gene is a G-protein coupled receptor. The protein has three transcript variants in humans.[11] Of these three, GPR113 Variant 1 has the longest amino acid sequence, and has the highest identity to orthologs. This leads to the conclusion that GPR113 Variant 1 is the homo sapiens descendant of the ancestral GPR113 gene. GPR113 Var 1 contains 1079 Amino Acids, and is integral to the plasma membrane.[12] The 7-pass receptor contains 4 domains highlighted in the figure at right: Signal Peptide (Red), Hormone Receptor Domain (Blue), Latrophilin/CL-1-like GPS domain (Orange), and the 7-transmembrane receptor (Purple). Between the Hormone Receptor Domain and the GPS is a Domain of unknown function that is not highlighted.

Function

GPR113 is a G protein-coupled receptor that is involved in a neuropeptide signaling pathway.[12]

Expression & Disease

GPR113 has been found to be expressed differentially under diseased conditions. Under the condition of Type 2 diabetes, the percentile rank relative to other transcripts decreases relative to normal cell function.[13] The deletion of TP63, which mediates a wide variety of important body processes, also produces decreased GPR113 expression.[14] In mice brains, the cerebellum and the olfactory bulb both show transcription of the GPR113 gene.[15] Additionally, a study from the National Institute of Deafness and Other Communication Disorders has identified GPR113 expression to be highly restricted to a subset of taste receptor cells.[16] This paper's conclusions, coupled with olfactory bulb expression levels, could provide an avenue for future research, potentially illuminating more about GPR113's function.

Interacting Proteins

GPR113 has been shown to associate with the orphan G protein-coupled receptor GPR123.[17]

Transcription Factors

Top 10 Transcription factors of highest likelihood[18]
Binding site for S8 type homeodomains
Binding site for S8 type homeodomains
DLX-1, -2, and -5 binding sites
TCF/LEF-1 (secondary DNA binding preference)
Homeobox containing germ cell-specific transcription factor NOBOX
Spalt-like transcription factor 1
Transcriptional repressor CDP
Alternative splicing variant of FOXP1, activated in ESCs
Binding site for S8 type homeodomains
Homeobox containing germ cell-specific transcription factor NOBOX

Clinical significance

The clinical significance of this protein has not been established. However, the expression profiles provide exciting directions for future research of the GPR113 gene, especially in fields studying taste and smell.

References

1. GRCh38: Ensembl release 89: ENSG00000173567 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000067642 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Fredriksson R, Lagerstrom MC, Hoglund PJ, Schioth HB (November 2002). "Novel human G protein-coupled receptors with long N-terminals containing GPS domains and Ser/Thr-rich regions". FEBS Lett. 531 (3): 407–14. doi:10.1016/S0014-5793(02)03574-3. PMID 12435584.
6. "Entrez Gene: GPR113 G protein-coupled receptor 113".
7. "Homo sapiens chromosome 2, GRCh37.p13 Primary Assembly". 2013-08-13. Cite journal requires |journal= (help)
8. https://www.genecards.org/cgi-bin/carddisp.pl?gene=GPR113
9. "BLAST: Basic Local Alignment Search Tool".
10. "SDSC Biology Workbench".
11. "Homo sapiens adhesion G protein-coupled receptor F3 (ADGRF3), transcript variant 2, mRNA". 2018-06-24. Cite journal requires |journal= (help)
12. "ADGRF3 - Adhesion G-protein coupled receptor F3 precursor - Homo sapiens (Human) - ADGRF3 gene & protein".
13. "71224040 - GEO Profiles - NCBI".
14. "34655140 - GEO Profiles - NCBI".
15. "Gene Detail :: Allen Brain Atlas: Mouse Brain".
16. Lopezjimenez, N. D.; Sainz, E; Cavenagh, M. M.; Cruz-Ithier, M. A.; Blackwood, C. A.; Battey, J. F.; Sullivan, S. L. (2005). "Two novel genes, Gpr113, which encodes a family 2 G-protein-coupled receptor, and Trcg1, are selectively expressed in taste receptor cells". Genomics. 85 (4): 472–82. doi:10.1016/j.ygeno.2004.12.005. PMID 15780750.
17. "STRING: Functional protein association networks".
18. "ElDorado Introduction".

Further reading

• Takeda S, Kadowaki S, Haga T, et al. (2002). "Identification of G protein-coupled receptor genes from the human genome sequence". FEBS Lett. 520 (1–3): 97–101. doi:10.1016/S0014-5793(02)02775-8. PMID 12044878.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Vassilatis DK, Hohmann JG, Zeng H, et al. (2003). "The G protein-coupled receptor repertoires of human and mouse". Proc. Natl. Acad. Sci. U.S.A. 100 (8): 4903–8. Bibcode:2003PNAS..100.4903V. doi:10.1073/pnas.0230374100. PMC 153653. PMID 12679517.
• Clark HF, Gurney AL, Abaya E, et al. (2003). "The Secreted Protein Discovery Initiative (SPDI), a Large-Scale Effort to Identify Novel Human Secreted and Transmembrane Proteins: A Bioinformatics Assessment". Genome Res. 13 (10): 2265–70. doi:10.1101/gr.1293003. PMC 403697. PMID 12975309.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Bjarnadóttir TK, Fredriksson R, Höglund PJ, et al. (2005). "The human and mouse repertoire of the adhesion family of G-protein-coupled receptors". Genomics. 84 (1): 23–33. doi:10.1016/j.ygeno.2003.12.004. PMID 15203201.