TPCN2

Two pore segment channel 2 (TPC2) is a protein which in humans is encoded by the TPCN2 gene.[5] TPC2 is an ion channel, however, in contrast to other calcium and sodium channels which have four homologous domains, each containing 6 transmembrane segments (S1 to S6), TPCN1 only contains two domain (each containing segments S1 to S6).[6]

TPCN2
Identifiers
Aliases	TPCN2, SHEP10, TPC2, two pore segment channel 2
External IDs	OMIM: 612163 MGI: 2385297 HomoloGene: 16375 GeneCards: TPCN2
Gene location (Human) Chr. Chromosome 11 (human)[1] Band 11q13.3 Start 69,048,932 bp[1] End 69,136,316 bp[1]
Gene location (Mouse) Chr. Chromosome 7 (mouse)[2] Band 7|7 F5 Start 145,186,524 bp[2] End 145,284,011 bp[2]
Gene ontology Molecular function • calcium channel activity • voltage-gated ion channel activity • ion channel activity • GO:0001948 protein binding • identical protein binding • protein kinase binding • NAADP-sensitive calcium-release channel activity • voltage-gated calcium channel activity Cellular component • integral component of membrane • membrane • cell membrane • endosome membrane • lysosome • lysosomal membrane Biological process • release of sequestered calcium ion into cytosol • membrane depolarization during action potential • response to vitamin D • regulation of ion transmembrane transport • cellular calcium ion homeostasis • ion transport • ion transmembrane transport • regulation of autophagy • calcium ion transmembrane transport • lysosome organization • calcium-mediated signaling • calcium ion transport • transmembrane transport • smooth muscle contraction Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	219931	233979
Ensembl	ENSG00000162341	ENSMUSG00000048677
UniProt	Q8NHX9	Q8BWC0
RefSeq (mRNA)	NM_139075	NM_146206
RefSeq (protein)	NP_620714	NP_666318
Location (UCSC)	Chr 11: 69.05 – 69.14 Mb	Chr 7: 145.19 – 145.28 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Structure

TPC2 is homologous to TPC1, the best characterized member of the TPC family. The structure of a TPC1 ortholog from Arabidopsis thaliana has been solved by two laboratories.[7][8] The structures were solved using X-ray crystallography and contained the fold of a voltage-gated ion channel and EF hands.

Filoviral Infections

Genetic knockout and pharmacological inhibition experiments demonstrate that Two-pore Channels, TPC1 and TPC2, are required for infection by Filoviruses Ebola and Marburg in mice.[9]

See also

• Two-pore channel

References

1. GRCh38: Ensembl release 89: ENSG00000162341 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000048677 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. "Entrez Gene: two pore segment channel 2".
6. Clapham DE, Garbers DL (December 2005). "International Union of Pharmacology. L. Nomenclature and structure-function relationships of CatSper and two-pore channels". Pharmacological Reviews. 57 (4): 451–4. doi:10.1124/pr.57.4.7. PMID 16382101.
7. Guo J, Zeng W, Chen Q, Lee C, Chen L, Yang Y, Cang C, Ren D, Jiang Y (March 2016). "Structure of the voltage-gated two-pore channel TPC1 from Arabidopsis thaliana". Nature. 531 (7593): 196–201. doi:10.1038/nature16446. PMC 4841471. PMID 26689363.
8. Kintzer AF, Stroud RM (March 2016). "Structure, inhibition and regulation of two-pore channel TPC1 from Arabidopsis thaliana". Nature. 531 (7593): 258–62. doi:10.1038/nature17194. PMC 4863712. PMID 26961658.
9. Sakurai Y, Kolokoltsov AA, Chen CC, Tidwell MW, Bauta WE, Klugbauer N, Grimm C, Wahl-Schott C, Biel M, Davey RA (February 2015). "Ebola virus. Two-pore channels control Ebola virus host cell entry and are drug targets for disease treatment". Science. 347 (6225): 995–8. doi:10.1126/science.1258758. PMC 4550587. PMID 25722412.

Further reading

• Sulem P, Gudbjartsson DF, Stacey SN, Helgason A, Rafnar T, Jakobsdottir M, Steinberg S, Gudjonsson SA, Palsson A, Thorleifsson G, Pálsson S, Sigurgeirsson B, Thorisdottir K, Ragnarsson R, Benediktsdottir KR, Aben KK, Vermeulen SH, Goldstein AM, Tucker MA, Kiemeney LA, Olafsson JH, Gulcher J, Kong A, Thorsteinsdottir U, Stefansson K (July 2008). "Two newly identified genetic determinants of pigmentation in Europeans". Nature Genetics. 40 (7): 835–7. doi:10.1038/ng.160. PMID 18488028.
• Suzuki Y, Yamashita R, Shirota M, Sakakibara Y, Chiba J, Mizushima-Sugano J, Nakai K, Sugano S (September 2004). "Sequence comparison of human and mouse genes reveals a homologous block structure in the promoter regions". Genome Research. 14 (9): 1711–8. doi:10.1101/gr.2435604. PMC 515316. PMID 15342556.
• Calcraft PJ, Ruas M, Pan Z, Cheng X, Arredouani A, Hao X, Tang J, Rietdorf K, Teboul L, Chuang KT, Lin P, Xiao R, Wang C, Zhu Y, Lin Y, Wyatt CN, Parrington J, Ma J, Evans AM, Galione A, Zhu MX (May 2009). "NAADP mobilizes calcium from acidic organelles through two-pore channels". Nature. 459 (7246): 596–600. doi:10.1038/nature08030. PMC 2761823. PMID 19387438.
• Taylor TD, Noguchi H, Totoki Y, Toyoda A, Kuroki Y, Dewar K, Lloyd C, Itoh T, Takeda T, Kim DW, She X, Barlow KF, Bloom T, Bruford E, Chang JL, Cuomo CA, Eichler E, FitzGerald MG, Jaffe DB, LaButti K, Nicol R, Park HS, Seaman C, Sougnez C, Yang X, Zimmer AR, Zody MC, Birren BW, Nusbaum C, Fujiyama A, Hattori M, Rogers J, Lander ES, Sakaki Y (March 2006). "Human chromosome 11 DNA sequence and analysis including novel gene identification". Nature. 440 (7083): 497–500. doi:10.1038/nature04632. PMID 16554811.
• Fu GK, Wang JT, Yang J, Au-Young J, Stuve LL (July 2004). "Circular rapid amplification of cDNA ends for high-throughput extension cloning of partial genes". Genomics. 84 (1): 205–10. doi:10.1016/j.ygeno.2004.01.011. PMID 15203218.

External links

• TPCN2+protein,+human at the US National Library of Medicine Medical Subject Headings (MeSH)