PRKG1

cGMP-dependent protein kinase 1, alpha isozyme is an enzyme that in humans is encoded by the PRKG1 gene.[5][6][7]

PRKG1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1ZXA, 3OCP, 3OD0, 3OGJ, 4KU7, 4KU8, 4QX5, 4QXK, 4R4L, 4R4M, 4Z07
Identifiers
Aliases	PRKG1, AAT8, PKG, PRKG1B, PRKGR1B, cGK, cGK 1, cGK1, cGKI, cGKI-BETA, cGKI-alpha, protein kinase, cGMP-dependent, type I, PKG1, protein kinase cGMP-dependent 1
External IDs	OMIM: 176894 MGI: 108174 HomoloGene: 55964 GeneCards: PRKG1
Gene location (Human) Chr. Chromosome 10 (human)[1] Band 10q11.23-q21.1 Start 50,990,888 bp[1] End 52,298,423 bp[1]
Gene location (Mouse) Chr. Chromosome 19 (mouse)[2] Band 19|19 C1 Start 30,564,489 bp[2] End 31,765,033 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • transferase activity • nucleotide binding • calcium channel regulator activity • cGMP binding • kinase activity • GO:0001948 protein binding • catalytic activity • ATP binding • protein serine/threonine kinase activity • cGMP-dependent protein kinase activity • identical protein binding • protein kinase activity Cellular component • cytoplasm • cytosol • Golgi apparatus • cell membrane Biological process • negative regulation of smooth muscle contraction • phosphorylation • GO:0043087, GO:0032313, GO:0032319, GO:0032314, GO:0043088 regulation of GTPase activity • dendrite development • neuron migration • negative regulation of platelet aggregation • cGMP-mediated signaling • relaxation of vascular smooth muscle • forebrain development • metabolism • actin cytoskeleton organization • signal transduction • protein phosphorylation • negative regulation of vascular smooth muscle cell proliferation • negative regulation of vascular associated smooth muscle cell migration Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	5592	19091
Ensembl	ENSG00000185532	ENSMUSG00000052920
UniProt	Q13976	P0C605
RefSeq (mRNA)	NM_001098512 NM_006258 NM_001374781 NM_001374782	NM_001013833 NM_011160
RefSeq (protein)	NP_001091982 NP_006249 NP_001361710 NP_001361711	NP_001013855 NP_035290
Location (UCSC)	Chr 10: 50.99 – 52.3 Mb	Chr 19: 30.56 – 31.77 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Interactions

PRKG1 has been shown to interact with:

• GTF2I,[8]
• ITPR1,[9]
• MRVI1,[9][10]
• RGS2,[11] and
• TNNT1.[12]

References

1. GRCh38: Ensembl release 89: ENSG00000185532 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000052920 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Sandberg M, Natarajan V, Ronander I, Kalderon D, Walter U, Lohmann SM, Jahnsen T (Sep 1989). "Molecular cloning and predicted full-length amino acid sequence of the type I beta isozyme of cGMP-dependent protein kinase from human placenta. Tissue distribution and developmental changes in rat". FEBS Letters. 255 (2): 321–9. doi:10.1016/0014-5793(89)81114-7. PMID 2792381.
6. Orstavik S, Sandberg M, Bérubé D, Natarajan V, Simard J, Walter U, Gagné R, Hansson V, Jahnsen T (April 1992). "Localization of the human gene for the type I cyclic GMP-dependent protein kinase to chromosome 10". Cytogenetics and Cell Genetics. 59 (4): 270–3. doi:10.1159/000133267. PMID 1544322.
7. "Entrez Gene: PRKG1 protein kinase, cGMP-dependent, type I".
8. Casteel DE, Zhuang S, Gudi T, Tang J, Vuica M, Desiderio S, Pilz RB (Aug 2002). "cGMP-dependent protein kinase I beta physically and functionally interacts with the transcriptional regulator TFII-I". The Journal of Biological Chemistry. 277 (35): 32003–14. doi:10.1074/jbc.M112332200. PMID 12082086.
9. Schlossmann J, Ammendola A, Ashman K, Zong X, Huber A, Neubauer G, Wang GX, Allescher HD, Korth M, Wilm M, Hofmann F, Ruth P (Mar 2000). "Regulation of intracellular calcium by a signalling complex of IRAG, IP3 receptor and cGMP kinase Ibeta". Nature. 404 (6774): 197–201. doi:10.1038/35004606. PMID 10724174.
10. Ammendola A, Geiselhöringer A, Hofmann F, Schlossmann J (Jun 2001). "Molecular determinants of the interaction between the inositol 1,4,5-trisphosphate receptor-associated cGMP kinase substrate (IRAG) and cGMP kinase Ibeta". The Journal of Biological Chemistry. 276 (26): 24153–9. doi:10.1074/jbc.M101530200. PMID 11309393.
11. Tang KM, Wang GR, Lu P, Karas RH, Aronovitz M, Heximer SP, Kaltenbronn KM, Blumer KJ, Siderovski DP, Zhu Y, Mendelsohn ME, Tang M, Wang G (Dec 2003). "Regulator of G-protein signaling-2 mediates vascular smooth muscle relaxation and blood pressure". Nature Medicine. 9 (12): 1506–12. doi:10.1038/nm958. PMID 14608379.
12. Yuasa K, Michibata H, Omori K, Yanaka N (Dec 1999). "A novel interaction of cGMP-dependent protein kinase I with troponin T". The Journal of Biological Chemistry. 274 (52): 37429–34. doi:10.1074/jbc.274.52.37429. PMID 10601315.

Further reading

• Picciotto MR, Cohn JA, Bertuzzi G, Greengard P, Nairn AC (Jun 1992). "Phosphorylation of the cystic fibrosis transmembrane conductance regulator". The Journal of Biological Chemistry. 267 (18): 12742–52. PMID 1377674.
• Thomas MK, Francis SH, Corbin JD (Sep 1990). "Substrate- and kinase-directed regulation of phosphorylation of a cGMP-binding phosphodiesterase by cGMP". The Journal of Biological Chemistry. 265 (25): 14971–8. PMID 2168396.
• Pöhler D, Butt E, Meissner J, Müller S, Lohse M, Walter U, Lohmann SM, Jarchau T (Nov 1995). "Expression, purification, and characterization of the cGMP-dependent protein kinases I beta and II using the baculovirus system". FEBS Letters. 374 (3): 419–25. doi:10.1016/0014-5793(95)01168-E. PMID 7589584.
• Komalavilas P, Lincoln TM (Mar 1994). "Phosphorylation of the inositol 1,4,5-trisphosphate receptor by cyclic GMP-dependent protein kinase". The Journal of Biological Chemistry. 269 (12): 8701–7. PMID 8132598.
• Butt E, Abel K, Krieger M, Palm D, Hoppe V, Hoppe J, Walter U (May 1994). "cAMP- and cGMP-dependent protein kinase phosphorylation sites of the focal adhesion vasodilator-stimulated phosphoprotein (VASP) in vitro and in intact human platelets". The Journal of Biological Chemistry. 269 (20): 14509–17. PMID 8182057.
• Suko J, Maurer-Fogy I, Plank B, Bertel O, Wyskovsky W, Hohenegger M, Hellmann G (Jan 1993). "Phosphorylation of serine 2843 in ryanodine receptor-calcium release channel of skeletal muscle by cAMP-, cGMP- and CaM-dependent protein kinase". Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 1175 (2): 193–206. doi:10.1016/0167-4889(93)90023-I. PMID 8380342.
• Tamura N, Itoh H, Ogawa Y, Nakagawa O, Harada M, Chun TH, Suga S, Yoshimasa T, Nakao K (Mar 1996). "cDNA cloning and gene expression of human type Ialpha cGMP-dependent protein kinase". Hypertension. 27 (3 Pt 2): 552–7. doi:10.1161/01.hyp.27.3.552. PMID 8613202.
• Salih E, Zhou HY, Glimcher MJ (Jul 1996). "Phosphorylation of purified bovine bone sialoprotein and osteopontin by protein kinases". The Journal of Biological Chemistry. 271 (28): 16897–905. doi:10.1074/jbc.271.28.16897. PMID 8663267.
• Xing J, Ginty DD, Greenberg ME (Aug 1996). "Coupling of the RAS-MAPK pathway to gene activation by RSK2, a growth factor-regulated CREB kinase". Science. 273 (5277): 959–63. doi:10.1126/science.273.5277.959. PMID 8688081.
• Orstavik S, Natarajan V, Taskén K, Jahnsen T, Sandberg M (Jun 1997). "Characterization of the human gene encoding the type I alpha and type I beta cGMP-dependent protein kinase (PRKG1)". Genomics. 42 (2): 311–8. doi:10.1006/geno.1997.4743. PMID 9192852.
• Deak M, Clifton AD, Lucocq LM, Alessi DR (Aug 1998). "Mitogen- and stress-activated protein kinase-1 (MSK1) is directly activated by MAPK and SAPK2/p38, and may mediate activation of CREB". The EMBO Journal. 17 (15): 4426–41. doi:10.1093/emboj/17.15.4426. PMC 1170775. PMID 9687510.
• Du K, Montminy M (Dec 1998). "CREB is a regulatory target for the protein kinase Akt/PKB". The Journal of Biological Chemistry. 273 (49): 32377–9. doi:10.1074/jbc.273.49.32377. PMID 9829964.
• Endo S, Suzuki M, Sumi M, Nairn AC, Morita R, Yamakawa K, Greengard P, Ito M (Mar 1999). "Molecular identification of human G-substrate, a possible downstream component of the cGMP-dependent protein kinase cascade in cerebellar Purkinje cells". Proceedings of the National Academy of Sciences of the United States of America. 96 (5): 2467–72. doi:10.1073/pnas.96.5.2467. PMC 26808. PMID 10051666.
• Wang X, Bruderer S, Rafi Z, Xue J, Milburn PJ, Krämer A, Robinson PJ (Aug 1999). "Phosphorylation of splicing factor SF1 on Ser20 by cGMP-dependent protein kinase regulates spliceosome assembly". The EMBO Journal. 18 (16): 4549–59. doi:10.1093/emboj/18.16.4549. PMC 1171529. PMID 10449420.
• Surks HK, Mochizuki N, Kasai Y, Georgescu SP, Tang KM, Ito M, Lincoln TM, Mendelsohn ME (Nov 1999). "Regulation of myosin phosphatase by a specific interaction with cGMP- dependent protein kinase Ialpha". Science. 286 (5444): 1583–7. doi:10.1126/science.286.5444.1583. PMID 10567269.
• Yuasa K, Michibata H, Omori K, Yanaka N (Dec 1999). "A novel interaction of cGMP-dependent protein kinase I with troponin T". The Journal of Biological Chemistry. 274 (52): 37429–34. doi:10.1074/jbc.274.52.37429. PMID 10601315.
• Yuasa K, Omori K, Yanaka N (Feb 2000). "Binding and phosphorylation of a novel male germ cell-specific cGMP-dependent protein kinase-anchoring protein by cGMP-dependent protein kinase Ialpha". The Journal of Biological Chemistry. 275 (7): 4897–905. doi:10.1074/jbc.275.7.4897. PMID 10671526.
• Huber A, Neuhuber WL, Klugbauer N, Ruth P, Allescher HD (Feb 2000). "Cysteine-rich protein 2, a novel substrate for cGMP kinase I in enteric neurons and intestinal smooth muscle". The Journal of Biological Chemistry. 275 (8): 5504–11. doi:10.1074/jbc.275.8.5504. PMID 10681529.