PKN3 (gene)

PKN3 is a protein kinase C-related molecule and thought to be an effector mediating malignant cell growth downstream of activated phosphoinositide 3-kinase (PI3K).[5] It is thought that chronic activation of the phosphoinositide 3-kinase (PI3K)/PTEN signal transduction pathway contributes to metastatic cell growth and that PKN3 may mediate that growth.1

PKN3
Identifiers
Aliases	PKN3, UTDP4-1, protein kinase N3
External IDs	OMIM: 610714 MGI: 2388285 HomoloGene: 50980 GeneCards: PKN3
Gene location (Human) Chr. Chromosome 9 (human)[1] Band 9q34.11 Start 128,702,503 bp[1] End 128,720,916 bp[1]
Gene location (Mouse) Chr. Chromosome 2 (mouse)[2] Band 2|2 B Start 30,077,684 bp[2] End 30,091,022 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • transferase activity • protein kinase activity • nucleotide binding • protein serine/threonine kinase activity • protein kinase C activity • GO:0001948 protein binding • ATP binding • kinase activity • GTP-Rho binding Cellular component • cytoplasm • perinuclear region of cytoplasm • Golgi apparatus • cell nucleus Biological process • protein phosphorylation • GO:0007243 intracellular signal transduction • peptidyl-serine phosphorylation • epithelial cell migration • phosphorylation • signal transduction Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	29941	263803
Ensembl	ENSG00000160447	ENSMUSG00000026785
UniProt	Q6P5Z2	Q8K045
RefSeq (mRNA)	NM_013355 NM_001317926	NM_153805
RefSeq (protein)	NP_001304855 NP_037487	NP_722500
Location (UCSC)	Chr 9: 128.7 – 128.72 Mb	Chr 2: 30.08 – 30.09 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

PKN3 is required for invasive prostate cell growth as assessed by 3D cell culture assays and in an orthotopic mouse tumor model by inducible expression of short hairpin RNA (shRNA). PKN3 may represent a target for therapeutic intervention in cancers that lack tumor suppressor PTEN function or depend on chronic activation of PI3K.

Interactions

PKN3 (gene) has been shown to interact with ARHGAP26.[6]

References

1. GRCh38: Ensembl release 89: ENSG00000160447 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000026785 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. "Entrez Gene: PKN3 protein kinase N3".
6. Shibata H, Oishi K, Yamagiwa A, Matsumoto M, Mukai H, Ono Y (July 2001). "PKNbeta interacts with the SH3 domains of Graf and a novel Graf related protein, Graf2, which are GTPase activating proteins for Rho family". Journal of Biochemistry. 130 (1): 23–31. doi:10.1093/oxfordjournals.jbchem.a002958. PMID 11432776.

Further reading

• Wissing J, Jänsch L, Nimtz M, Dieterich G, Hornberger R, Kéri G, Wehland J, Daub H (March 2007). "Proteomics analysis of protein kinases by target class-selective prefractionation and tandem mass spectrometry". Molecular & Cellular Proteomics. 6 (3): 537–47. doi:10.1074/mcp.T600062-MCP200. PMID 17192257.
• Leenders F, Möpert K, Schmiedeknecht A, Santel A, Czauderna F, Aleku M, Penschuck S, Dames S, Sternberger M, Röhl T, Wellmann A, Arnold W, Giese K, Kaufmann J, Klippel A (August 2004). "PKN3 is required for malignant prostate cell growth downstream of activated PI 3-kinase". The EMBO Journal. 23 (16): 3303–13. doi:10.1038/sj.emboj.7600345. PMC 514518. PMID 15282551.
• Shibata H, Oishi K, Yamagiwa A, Matsumoto M, Mukai H, Ono Y (July 2001). "PKNbeta interacts with the SH3 domains of Graf and a novel Graf related protein, Graf2, which are GTPase activating proteins for Rho family". Journal of Biochemistry. 130 (1): 23–31. doi:10.1093/oxfordjournals.jbchem.a002958. PMID 11432776.
• Oishi K, Takahashi M, Mukai H, Banno Y, Nakashima S, Kanaho Y, Nozawa Y, Ono Y (May 2001). "PKN regulates phospholipase D1 through direct interaction". The Journal of Biological Chemistry. 276 (21): 18096–101. doi:10.1074/jbc.M010646200. PMID 11259428.
• Oishi K, Mukai H, Shibata H, Takahashi M, Ona Y (August 1999). "Identification and characterization of PKNbeta, a novel isoform of protein kinase PKN: expression and arachidonic acid dependency are different from those of PKNalpha". Biochemical and Biophysical Research Communications. 261 (3): 808–14. doi:10.1006/bbrc.1999.1116. PMID 10441506.