CKAP5

Cytoskeleton-associated protein 5 is a microtubule-associated protein that in humans is encoded by the CKAP5 gene.[5][6][7] It is the homolog of the Xenopus protein XMAP215[8] and is also known as ch-Tog.

CKAP5
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 4QMI, 4QMJ
Identifiers
Aliases	CKAP5, CHTOG, MSPS, TOG, TOGp, ch-TOG, cytoskeleton associated protein 5
External IDs	OMIM: 611142 MGI: 1923036 HomoloGene: 8844 GeneCards: CKAP5
Gene location (Human) Chr. Chromosome 11 (human)[1] Band 11p11.2 Start 46,743,048 bp[1] End 46,846,308 bp[1]
Gene location (Mouse) Chr. Chromosome 2 (mouse)[2] Band 2|2 E1 Start 91,526,762 bp[2] End 91,620,664 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • GO:0001948 protein binding • cadherin binding • microtubule plus-end binding Cellular component • cytoplasm • cytosol • centrosome • spindle pole • membrane • microtubule cytoskeleton • gamma-tubulin complex • microtubule organizing center • microtubule plus-end • cytoskeleton • chromosome, centromeric region • kinetochore • condensed chromosome kinetochore • chromosome • spindle • nucleolus • cell membrane • macromolecular complex Biological process • RNA transport • establishment or maintenance of microtubule cytoskeleton polarity • cell division • GO:0043146 spindle organization • G2/M transition of mitotic cell cycle • cell cycle • sister chromatid cohesion • centrosome cycle • microtubule polymerization • centrosome duplication • ciliary basal body docking • regulation of G2/M transition of mitotic cell cycle • microtubule depolymerization Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	9793	75786
Ensembl	ENSG00000175216	ENSMUSG00000040549
UniProt	Q14008	A2AGT5
RefSeq (mRNA)	NM_014756 NM_001008938	NM_001165989 NM_029437
RefSeq (protein)	NP_001008938 NP_055571	NP_001159461 NP_083713
Location (UCSC)	Chr 11: 46.74 – 46.85 Mb	Chr 2: 91.53 – 91.62 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

It has at least two distinct roles in spindle formation: it protects kinetochore microtubules from depolymerization by MCAK (KIF2C), and ch-Tog plays an essential role in centrosomal microtubule assembly, a function independent of MCAK activity.[9]

Interactions

CKAP5 has been shown to interact with TACC1.[10][11]

References

1. GRCh38: Ensembl release 89: ENSG00000175216 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000040549 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Nagase T, Miyajima N, Tanaka A, Sazuka T, Seki N, Sato S, Tabata S, Ishikawa K, Kawarabayasi Y, Kotani H (Jul 1995). "Prediction of the coding sequences of unidentified human genes. III. The coding sequences of 40 new genes (KIAA0081-KIAA0120) deduced by analysis of cDNA clones from human cell line KG-1". DNA Res. 2 (1): 37–43. doi:10.1093/dnares/2.1.37. PMID 7788527.
6. Charrasse S, Mazel M, Taviaux S, Berta P, Chow T, Larroque C (Feb 1996). "Characterization of the cDNA and pattern of expression of a new gene over-expressed in human hepatomas and colonic tumors". Eur. J. Biochem. 234 (2): 406–13. doi:10.1111/j.1432-1033.1995.406_b.x. PMID 8536682.
7. "Entrez Gene: CKAP5 cytoskeleton associated protein 5".
8. Cassimeris L, Morabito J (2004). "TOGp, the human homolog of XMAP215/Dis1, is required for centrosome integrity, spindle pole organization, and bipolar spindle assembly". Mol. Biol. Cell. 15 (4): 1580–90. doi:10.1091/mbc.E03-07-0544. PMC 379257. PMID 14718566.
9. Barr AR, Gergely F (2008). "MCAK-independent functions of ch-Tog/XMAP215 in microtubule plus-end dynamics". Mol. Cell. Biol. 28 (23): 7199–211. doi:10.1128/MCB.01040-08. PMC 2593372. PMID 18809577.
10. Conte N, Delaval B, Ginestier C, Ferrand A, Isnardon D, Larroque C, Prigent C, Séraphin B, Jacquemier J, Birnbaum D (Nov 2003). "TACC1-chTOG-Aurora A protein complex in breast cancer". Oncogene. 22 (50): 8102–16. doi:10.1038/sj.onc.1206972. PMID 14603251.
11. Lauffart B, Howell SJ, Tasch JE, Cowell JK, Still IH (Apr 2002). "Interaction of the transforming acidic coiled-coil 1 (TACC1) protein with ch-TOG and GAS41/NuBI1 suggests multiple TACC1-containing protein complexes in human cells". Biochem. J. 363 (Pt 1): 195–200. doi:10.1042/0264-6021:3630195. PMC 1222467. PMID 11903063.

External links

• Human CKAP5 genome location and CKAP5 gene details page in the UCSC Genome Browser.

Further reading

• Basto R, Gergely F, Draviam VM, Ohkura H, Liley K, Raff JW (2007). "Hsp90 is required to localise cyclin B and Msps/ch-TOG to the mitotic spindle in Drosophila and humans". J. Cell Sci. 120 (Pt 7): 1278–87. doi:10.1242/jcs.000604. PMID 17376965.
• Kosturko LD, Maggipinto MJ, D'Sa C, Carson JH, Barbarese E (2005). "The microtubule-associated protein tumor overexpressed gene binds to the RNA trafficking protein heterogeneous nuclear ribonucleoprotein A2". Mol. Biol. Cell. 16 (4): 1938–47. doi:10.1091/mbc.E04-08-0709. PMC 1073673. PMID 15703215.
• Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
• Cassimeris L, Morabito J (2004). "TOGp, the human homolog of XMAP215/Dis1, is required for centrosome integrity, spindle pole organization, and bipolar spindle assembly". Mol. Biol. Cell. 15 (4): 1580–90. doi:10.1091/mbc.E03-07-0544. PMC 379257. PMID 14718566.
• Conte N, Delaval B, Ginestier C, Ferrand A, Isnardon D, Larroque C, Prigent C, Séraphin B, Jacquemier J, Birnbaum D (2003). "TACC1-chTOG-Aurora A protein complex in breast cancer". Oncogene. 22 (50): 8102–16. doi:10.1038/sj.onc.1206972. PMID 14603251.
• Gergely F, Draviam VM, Raff JW (2003). "The ch-TOG/XMAP215 protein is essential for spindle pole organization in human somatic cells". Genes Dev. 17 (3): 336–41. doi:10.1101/gad.245603. PMC 195983. PMID 12569123.
• Nakayama M, Kikuno R, Ohara O (2002). "Protein-protein interactions between large proteins: two-hybrid screening using a functionally classified library composed of long cDNAs". Genome Res. 12 (11): 1773–84. doi:10.1101/gr.406902. PMC 187542. PMID 12421765.
• Lauffart B, Howell SJ, Tasch JE, Cowell JK, Still IH (2002). "Interaction of the transforming acidic coiled-coil 1 (TACC1) protein with ch-TOG and GAS41/NuBI1 suggests multiple TACC1-containing protein complexes in human cells". Biochem. J. 363 (Pt 1): 195–200. doi:10.1042/0264-6021:3630195. PMC 1222467. PMID 11903063.
• Bonaldo MF, Lennon G, Soares MB (1996). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Res. 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548.
• Nakamura S, Grigoriev I, Nogi T, Hamaji T, Cassimeris L, Mimori-Kiyosue Y (2012). "Dissecting the nanoscale distributions and functions of microtubule-end-binding proteins EB1 and ch-TOG in interphase HeLa cells". PLoS ONE. 7 (12): e51442. doi:10.1371/journal.pone.0051442. PMC 3520847. PMID 23251535.
• Zanic M, Widlund PO, Hyman AA, Howard J (2013). "Synergy between XMAP215 and EB1 increases microtubule growth rates to physiological levels". Nat. Cell Biol. 15 (6): 688–93. doi:10.1038/ncb2744. PMID 23666085.