Phosphatidate cytidylyltransferase
Phosphatidate cytidylyltransferase (EC 2.7.7.41)[1][2][3] (also known as CDP- diacylglycerol synthase) (CDS) is the enzyme that catalyzes the synthesis of CDP-diacylglycerol from cytidine triphosphate and phosphatidate.
- CTP + phosphatidate diphosphate + CDP-diacylglycerol
| phosphatidate cytidylyltransferase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 2.7.7.41 | ||||||||
| CAS number | 9067-83-8 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
| Cytidylyltransferase family | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| Symbol | CTP_transf_1 | ||||||||
| Pfam | PF01148 | ||||||||
| InterPro | IPR000374 | ||||||||
| PROSITE | PDOC01019 | ||||||||
| OPM superfamily | 414 | ||||||||
| OPM protein | 4q2e | ||||||||
| |||||||||
Thus, the two substrates of this enzyme are cytidine triphosphate, or CTP, and phosphatidate, whereas its two products are diphosphate and CDP-diacylglycerol.
CDP-diacylglycerol is an important branch point intermediate in both prokaryotic and eukaryotic organisms. CDS is a membrane-bound enzyme.
This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing nucleotide groups (nucleotidyltransferases). The systematic name of this enzyme class is CTP:phosphatidate cytidylyltransferase. Other names in common use include CDP diglyceride pyrophosphorylase, CDP-diacylglycerol synthase, CDP-diacylglyceride synthetase, cytidine diphosphoglyceride pyrophosphorylase, phosphatidate cytidyltransferase, phosphatidic acid cytidylyltransferase, CTP:1,2-diacylglycerophosphate-cytidyl transferase, CTP-diacylglycerol synthetase, DAG synthetase, and CDP-DG. This enzyme participates in glycerophospholipid metabolism and phosphatidylinositol signaling system.
References
- Sparrow CP, Raetz CR (1985). "Purification and properties of the membrane-bound CDP-diglyceride synthetase from Escherichia coli". J. Biol. Chem. 260 (22): 12084–12091. PMID 2995359.
- Shen H, Heacock PN, Clancey CJ, Dowhan W (1996). "The CDS1 gene encoding CDP-diacylglycerol synthase in Saccharomyces cerevisiae is essential for cell growth". J. Biol. Chem. 271 (2): 789–795. doi:10.1074/jbc.271.2.789. PMID 8557688.
- Kondo H, Goto K, Saito S, Tonosaki A (1997). "Gene cloning and characterization of CDP-diacylglycerol synthase from rat brain". J. Biol. Chem. 272 (14): 9503–9509. doi:10.1074/jbc.272.14.9503. PMID 9083091.
- Carter JR, Kennedy EP (1966). "Enzymatic synthesis of cytidine diphosphate diglyceride". J. Lipid Res. 7 (5): 678–83. PMID 4291255.
- McCaman RE, Finnerty WR (1968). "Biosynthesis of cytidine diphosphate-diglyceride by a particulate fracgion from Micrococcus cerificans". J. Biol. Chem. 243 (19): 5074–80. PMID 5679981.
- Petzold GL, Agranoff BW (1967). "The biosynthesis of cytidine diphosphate diglyceride by embryonic chick brain". J. Biol. Chem. 242 (6): 1187–91. PMID 6067194.