PRIM1

DNA primase small subunit is an enzyme that in humans is encoded by the PRIM1 gene.[5]

PRIM1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 4BPU, 4BPW, 4BPX, 4LIK, 4LIL, 4MHQ, 4RR2, 5EXR
Identifiers
Aliases	PRIM1, p49, primase (DNA) subunit 1, DNA primase subunit 1
External IDs	OMIM: 176635 MGI: 97757 HomoloGene: 730 GeneCards: PRIM1
Gene location (Human) Chr. Chromosome 12 (human)[1] Band 12q13.3 Start 56,731,296 bp[1] End 56,752,374 bp[1]
Gene location (Mouse) Chr. Chromosome 10 (mouse)[2] Band 10 76.39 cM|10 D3 Start 128,015,168 bp[2] End 128,030,037 bp[2]
Gene ontology Molecular function • DNA-directed 5'-3' RNA polymerase activity • transferase activity • nucleotidyltransferase activity • metal ion binding • single-stranded DNA binding • DNA primase activity Cellular component • membrane • nucleoplasm • alpha DNA polymerase:primase complex • primosome complex Biological process • DNA replication • G1 phase • DNA replication initiation • DNA replication, synthesis of RNA primer • telomere maintenance via semi-conservative replication • G1/S transition of mitotic cell cycle Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	5557	19075
Ensembl	ENSG00000198056	ENSMUSG00000025395
UniProt	P49642	P20664
RefSeq (mRNA)	NM_000946	NM_008921
RefSeq (protein)	NP_000937	NP_032947
Location (UCSC)	Chr 12: 56.73 – 56.75 Mb	Chr 10: 128.02 – 128.03 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the small, 49 kDa primase subunit.

References

1. GRCh38: Ensembl release 89: ENSG00000198056 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000025395 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Shiratori A, Okumura K, Nogami M, Taguchi H, Onozaki T, Inoue T, Ando T, Shibata T, Izumi M, Miyazawa H, et al. (Feb 1996). "Assignment of the 49-kDa (PRIM1) and 58-kDa (PRIM2A and PRIM2B) subunit genes of the human DNA primase to chromosome bands 1q44 and 6p11.1-p12". Genomics. 28 (2): 350–3. doi:10.1006/geno.1995.1155. PMID 8530050.

Further reading

• Stadlbauer F, Brueckner A, Rehfuess C, et al. (1994). "DNA replication in vitro by recombinant DNA-polymerase-alpha-primase". Eur. J. Biochem. 222 (3): 781–93. doi:10.1111/j.1432-1033.1994.tb18925.x. PMID 8026492.
• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Schneider A, Smith RW, Kautz AR, et al. (1998). "Primase activity of human DNA polymerase alpha-primase. Divalent cations stabilize the enzyme activity of the p48 subunit". J. Biol. Chem. 273 (34): 21608–15. doi:10.1074/jbc.273.34.21608. PMID 9705292.
• Arezi B, Kirk BW, Copeland WC, Kuchta RD (1999). "Interactions of DNA with human DNA primase monitored with photoactivatable cross-linking agents: implications for the role of the p58 subunit". Biochemistry. 38 (39): 12899–907. doi:10.1021/bi9908991. PMID 10504261.
• Smith RW, Nasheuer HP (2002). "Control of complex formation of DNA polymerase alpha-primase and cell-free DNA replication by the C-terminal amino acids of the largest subunit p180". FEBS Lett. 527 (1–3): 143–6. doi:10.1016/S0014-5793(02)03197-6. PMID 12220650.

External links

• Overview of all the structural information available in the PDB for UniProt: P49642 (Human DNA primase small subunit (PRIM1)) at the PDBe-KB.