Prostacyclin

Prostacyclin (also called prostaglandin I2 or PGI₂) is a prostaglandin member of the eicosanoid family of lipid molecules. It inhibits platelet activation and is also an effective vasodilator.

Prostacyclin

Clinical data
AHFS/Drugs.com	Monograph
ATC code	B01AC09 (WHO)
Legal status
Legal status	AU: S4 (Prescription only)
Pharmacokinetic data
Elimination half-life	42 seconds
Identifiers
IUPAC name (Z)-5-[(4R,5R)-5-Hydroxy-4-((S,E)-3-hydroxyoct-1-enyl)hexahydro-2H-cyclopenta[b]furan-2-ylidene]pentanoic acid
CAS Number	35121-78-9^Y
PubChem CID	5282411
IUPHAR/BPS	1915
DrugBank	DB01240^N
ChemSpider	4445566^N
UNII	DCR9Z582X0
ChEMBL	ChEMBL962^N
CompTox Dashboard (EPA)	DTXSID5022988
Chemical and physical data
Formula	C20H32O5
Molar mass	352.471 g·mol^−1
3D model (JSmol)	Interactive image
SMILES OC(=O)CCC\C=C1\C[C@@H]2[C@@H](/C=C/[C@@H](O)CCCCC)[C@H](O)C[C@@H]2O1
InChI InChI=1S/C20H32O5/c1-2-3-4-7-14(21)10-11-16-17-12-15(8-5-6-9-20(23)24)25-19(17)13-18(16)22/h8,10-11,14,16-19,21-22H,2-7,9,12-13H2,1H3,(H,23,24)/b11-10+,15-8-/t14-,16+,17+,18+,19-/m0/s1^N Key:KAQKFAOMNZTLHT-OZUDYXHBSA-N^N
^NY (what is this?)  (verify)

When used as a drug, it is also known as epoprostenol.[1] The terms are sometimes used interchangeably.[2]

Function

Prostacyclin (PGI₂) chiefly prevents formation of the platelet plug involved in primary hemostasis (a part of blood clot formation). It does this by inhibiting platelet activation.[3] It is also an effective vasodilator. Prostacyclin's interactions contrast with those of thromboxane (TXA₂), another eicosanoid. These strongly suggest a mechanism of cardiovascular homeostasis between these two hormones in relation to vascular damage.

Medical uses

It is used to treat pulmonary arterial hypertension.[4]

Degradation

Prostacyclin, which has a half-life of 42 seconds,[5] is broken down into 6-keto-PGF₁, which is a much weaker vasodilator.

Mechanism

Prostacyclin effect		Mechanism	Cellular response
Classical functions	Vessel tone	↑cAMP, ↓ET-1 ↓Ca^2+, ↑K^+	↓SMC proliferation ↑Vasodilation
	Antiproliferative	↑cAMP ↑PPARgamma	↓Fibroblast growth ↑Apoptosis
	Antithrombotic	↓Thromboxane-A2 ↓PDGF	↓Platelet aggregation ↓Platelet adherence to vessel wall
Novel functions	Antiinflammatory	↓IL-1, IL-6 ↑IL-10	↓Proinflammatory cytokines ↑Antiinflammatory cytokines
	Antimitogenic	↓VEGF ↓TGF-β	↓Angiogenesis ↑ECM remodeling

As mentioned above, prostacyclin (PGI₂) is released by healthy endothelial cells and performs its function through a paracrine signaling cascade that involves G protein-coupled receptors on nearby platelets and endothelial cells. The platelet Gs protein-coupled receptor (prostacyclin receptor) is activated when it binds to PGI₂. This activation, in turn, signals adenylyl cyclase to produce cAMP. cAMP goes on to inhibit any undue platelet activation (in order to promote circulation) and also counteracts any increase in cytosolic calcium levels that would result from thromboxane A2 (TXA₂) binding (leading to platelet activation and subsequent coagulation). PGI₂ also binds to endothelial prostacyclin receptors, and in the same manner, raises cAMP levels in the cytosol. This cAMP then goes on to activate protein kinase A (PKA). PKA then continues the cascade by promoting the phosphorylation of the myosin light chain kinase, which inhibits it and leads to smooth muscle relaxation and vasodilation. It can be noted that PGI₂ and TXA₂ work as physiological antagonists.

Members[6]

PROSTACYCLINS
Flolan (epoprostenol sodium) for Injection	Continuously infused	2 ng/kg/min to start, increased by 2 ng/kg/min every 15 minutes or longer until suitable efficacy/tolerability balance is achieved	Class III Class IV
Veletri (epoprostenol) for Injection	Continuously infused	2 ng/kg/min to start, increased by 2 ng/kg/min every 15 minutes or longer until suitable efficacy/tolerability balance is achieved	Class III Class IV
Remodulin SC§ (treprostinil sodium) Injection	Continuously infused	1.25 ng/kg/min to start, increased by up to 1.25 ng/kg/min per week for 4 weeks, then up to 2.5 ng/kg/min per week until suitable efficacy/tolerability balance is achieved	Class II Class III Class IV
Ventavis (iloprost) Inhalation Solution	Inhaled 6–9 times daily	2.5 μg 6–9 times daily to start, increased to 5.0 μg 6–9 times daily if well tolerated	Class III Class IV

Pharmacology

Ball-and-stick model of prostacyclin

Synthetic prostacyclin analogues (iloprost, cisaprost) are used intravenously, subcutaneously or by inhalation:

• as a vasodilator in severe Raynaud's phenomenon or ischemia of a limb;
• in pulmonary hypertension.
• in primary pulmonary hypertension (PPH)

The production of prostacyclin is inhibited by the action of NSAIDs on cyclooxygenase enzymes COX1 and COX2. These convert arachidonic acid to prostaglandin H2 (PGH₂), the immediate precursor of prostacyclin. Since thromboxane (an eicosanoid stimulator of platelet aggregation) is also downstream of COX enzymes, one might think that the effect of NSAIDs would act to balance. However, prostacyclin concentrations recover much faster than thromboxane levels, so aspirin administration initially has little to no effect but eventually prevents platelet aggregation (the effect of prostaglandins predominates as they are regenerated). This is explained by understanding the cells that produce each molecule, TXA₂ and PGI₂. Since PGI₂ is primarily produced in a nucleated endothelial cell, the COX inhibition by NSAID can be overcome with time by increased COX gene activation and subsequent production of more COX enzymes to catalyze the formation of PGI₂. In contrast, TXA₂ is released primarily by anucleated platelets, which are unable to respond to NSAID COX inhibition with additional transcription of the COX gene because they lack DNA material necessary to perform such a task. This allows NSAIDs to result in PGI₂ dominance that promotes circulation and retards thrombosis.

In patients with pulmonary hypertension, inhaled epoprostenol reduces pulmonary pressure, and improves right ventricular stroke volume in patients undergoing cardiac surgery. A dose of 60 μg is hemodynamically safe, and its effect is completely reversed after 25 minutes. No evidence of platelet dysfunction or an increase in surgical bleeding after administration of inhaled epoprostenol has been found.[7] The drug has been known to cause flushing, headaches and hypotension.[8]

Synthesis

Biosynthesis

Eicosanoid synthesis. (Prostacyclin near bottom center.)

Prostacyclin is produced in endothelial cells, which line the walls of arteries and veins,[9] from prostaglandin H₂ (PGH₂) by the action of the enzyme prostacyclin synthase. Although prostacyclin is considered an independent mediator, it is called PGI₂ (prostaglandin I₂) in eicosanoid nomenclature, and is a member of the prostanoids (together with the prostaglandins and thromboxane). It is important to note that COX 1 has been found to be the major prostacyclin producing cyclooxygenase in the endothelial cells of the blood vessels. [10]

The series-3 prostaglandin PGH₃ also follows the prostacyclin synthase pathway, yielding another prostacyclin, PGI₃.[11] The unqualified term 'prostacyclin' usually refers to PGI₂. PGI₂ is derived from the ω-6 arachidonic acid. PGI₃ is derived from the ω-3 EPA.

Artificial synthesis

Prostacyclin can be synthesized from the methyl ester of prostaglandin F_2α.[12] After its synthesis, the drug is reconstituted in saline and glycerin.[13]

History

During the 1960s, a UK research team, headed by Professor John Vane, began to explore the role of prostaglandins in anaphylaxis and respiratory diseases. Working with a team from the Royal College of Surgeons, Vane discovered that aspirin and other oral anti-inflammatory drugs work by inhibiting the synthesis of prostaglandins. This critical finding opened the door to a broader understanding of the role of prostaglandins in the body.

A team at The Wellcome Foundation led by Salvador Moncada had identified a lipid mediator they called "PG-X," which inhibits platelet aggregation. PG-X, later known as prostacyclin, is 30 times more potent than any other then-known anti-aggregatory agent.

In 1976, Vane and fellow researchers Salvador Moncada, Ryszard Gryglewski, and Stuart Bunting published the first paper on prostacyclin in Nature.[14] The collaboration produced a synthesized molecule, which was named epoprostenol. But, as with native prostacyclin, the epoprostenol molecule is unstable in solution and prone to rapid degradation. This presented a challenge for both in vitro experiments and clinical applications.

To overcome this challenge, the research team that discovered prostacyclin continued the research. The research team synthesized nearly 1,000 analogues.

See also

• Essential fatty acid

References

1. "epoprostenol" at Dorland's Medical Dictionary
2. Kermode J, Butt W, Shann F (August 1991). "Comparison between prostaglandin E1 and epoprostenol (prostacyclin) in infants after heart surgery". British Heart Journal. 66 (2): 175–8. doi:10.1136/hrt.66.2.175. PMC 1024613. PMID 1883670.
3. Pathologic Basis of Disease, Robbins and Cotran, 8th ed. Saunders Philadelphia 2010
4. "Iloprost Monograph for Professionals". AHFS. Retrieved 29 December 2017.
5. Cawello, W; Schweer, H; Müller, R; Bonn, R; Seyberth, HW (1994). "Metabolism and pharmacokinetics of prostaglandin E1 administered by intravenous infusion in human subjects". European Journal of Clinical Pharmacology. 46 (3): 275–7. doi:10.1007/BF00192562. PMID 8070511.
6. ^ REM_RefGuideWC_AUG07v.1
7. Haché, M; Denault, A; Bélisle, S; Robitaille, D; Couture, P; Sheridan, P; Pellerin, M; Babin, D; Noël, N; Guertin, MC; Martineau, R; Dupuis, J (Mar 2003). "Inhaled epoprostenol (prostacyclin) and pulmonary hypertension before cardiac surgery". The Journal of Thoracic and Cardiovascular Surgery. 125 (3): 642–9. doi:10.1067/mtc.2003.107. PMID 12658208.
8. Nickson, C. (2015, October 28). Prostacyclin or Epoprostenol. Retrieved November 16, 2015, from http://lifeinthefastlane.com/ccc/prostacyclin-or-epoprostenol/
9. prostacyclin. (n.d.) Miller-Keane Encyclopedia and Dictionary of Medicine, Nursing, and Allied Health, Seventh Edition. (2003). Retrieved November 17, 2015 from http://medical-dictionary.thefreedictionary.com/prostacyclin
10. Kirkby, Nicholas S.; Lundberg, Martina H.; Harrington, Louise S.; Leadbeater, Philip D. M.; Milne, Ginger L.; Potter, Claire M. F.; Al-Yamani, Malak; Adeyemi, Oladipupo; Warner, Timothy D.; Mitchell, Jane A. (2012-10-23). "Cyclooxygenase-1, not cyclooxygenase-2, is responsible for physiological production of prostacyclin in the cardiovascular system". Proceedings of the National Academy of Sciences of the United States of America. 109 (43): 17597–17602. doi:10.1073/pnas.1209192109. ISSN 0027-8424. PMC 3491520. PMID 23045674.
11. Fischer S, Weber PC (1985). "Thromboxane (TX)A3 and prostaglandin (PG)I3 are formed in man after dietary eicosapentaenoic acid: identification and quantification by capillary gas chromatography-electron impact mass spectrometry". Biomed. Mass Spectrom. 12 (9): 470–6. doi:10.1002/bms.1200120905. PMID 2996649.
12. Johnson, Roy A.; Lincoln, Frank H.; Nidy, Eldon G.; Schneider, William P.; Thompson, John L.; Axen, Udo (1978). "Synthesis and characterization of prostacyclin, 6-ketoprostaglandin F1.alpha., prostaglandin I1, and prostaglandin I3". Journal of the American Chemical Society. 100 (24): 7690. doi:10.1021/ja00492a043.
13. Nickson, C. (2015, October 28). Prostacyclin or Epoprostenol. Retrieved November 16, 2015, from http://lifeinthefastlane.com/ccc/prostacyclin-or-epoprostenol/
14. Moncada, S.; Gryglewski, R.; Bunting, S.; Vane, J. R. (21 October 1976). "An enzyme isolated from arteries transforms prostaglandin endoperoxides to an unstable substance that inhibits platelet aggregation". Nature. 263 (5579): 663–665. Bibcode:1976Natur.263..663M. doi:10.1038/263663a0. PMID 802670.