TRNA-intron endonuclease
tRNA-intron lyase (EC 4.6.1.16, tRNA intron endonuclease, transfer ribonucleate intron endoribonuclease, tRNA splicing endonuclease, splicing endonuclease, tRNATRPintron endonuclease, transfer splicing endonuclease) is an enzyme.[1][2][3][4] This enzyme catalyses the following chemical reaction
- pre-tRNA a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
| tRNA-intron lyase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 4.6.1.16 | ||||||||
| CAS number | 117444-13-0 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
This enzyme catalyses the final stage in the maturation of tRNA molecules. Its systematic name is pretRNA lyase (intron-removing; cyclic-2',3'-phosphate-forming).
References
- Attardi DG, Margarit I, Tocchini-Valentini GP (December 1985). "Structural alterations in mutant precursors of the yeast tRNALeu3 gene which behave as defective substrates for a highly purified splicing endoribonuclease". The EMBO Journal. 4 (12): 3289–97. PMC 554656. PMID 3937725.
- Peebles CL, Gegenheimer P, Abelson J (February 1983). "Precise excision of intervening sequences from precursor tRNAs by a membrane-associated yeast endonuclease". Cell. 32 (2): 525–36. doi:10.1016/0092-8674(83)90472-5. PMID 6186398.
- Thompson LD, Brandon LD, Nieuwlandt DT, Daniels CJ (January 1989). "Transfer RNA intron processing in the halophilic archaebacteria". Canadian Journal of Microbiology. 35 (1): 36–42. doi:10.1139/m89-006. PMID 2470486.
- Thompson LD, Daniels CJ (December 1988). "A tRNA(Trp) intron endonuclease from Halobacterium volcanii. Unique substrate recognition properties". The Journal of Biological Chemistry. 263 (34): 17951–9. PMID 3192521.
External links
- TRNA-intron+endonuclease at the US National Library of Medicine Medical Subject Headings (MeSH)
This article is issued from Wikipedia. The text is licensed under Creative Commons - Attribution - Sharealike. Additional terms may apply for the media files.