TRNA-intron endonuclease

tRNA-intron lyase (EC 4.6.1.16, tRNA intron endonuclease, transfer ribonucleate intron endoribonuclease, tRNA splicing endonuclease, splicing endonuclease, tRNATRPintron endonuclease, transfer splicing endonuclease) is an enzyme.[1][2][3][4] This enzyme catalyses the following chemical reaction

pre-tRNA a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus
tRNA-intron lyase
Identifiers
EC number4.6.1.16
CAS number117444-13-0
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum

This enzyme catalyses the final stage in the maturation of tRNA molecules. Its systematic name is pretRNA lyase (intron-removing; cyclic-2',3'-phosphate-forming).

References

  1. Attardi DG, Margarit I, Tocchini-Valentini GP (December 1985). "Structural alterations in mutant precursors of the yeast tRNALeu3 gene which behave as defective substrates for a highly purified splicing endoribonuclease". The EMBO Journal. 4 (12): 3289–97. PMC 554656. PMID 3937725.
  2. Peebles CL, Gegenheimer P, Abelson J (February 1983). "Precise excision of intervening sequences from precursor tRNAs by a membrane-associated yeast endonuclease". Cell. 32 (2): 525–36. doi:10.1016/0092-8674(83)90472-5. PMID 6186398.
  3. Thompson LD, Brandon LD, Nieuwlandt DT, Daniels CJ (January 1989). "Transfer RNA intron processing in the halophilic archaebacteria". Canadian Journal of Microbiology. 35 (1): 36–42. doi:10.1139/m89-006. PMID 2470486.
  4. Thompson LD, Daniels CJ (December 1988). "A tRNA(Trp) intron endonuclease from Halobacterium volcanii. Unique substrate recognition properties". The Journal of Biological Chemistry. 263 (34): 17951–9. PMID 3192521.
This article is issued from Wikipedia. The text is licensed under Creative Commons - Attribution - Sharealike. Additional terms may apply for the media files.