Dirofilaria immitis

Dirofilaria immitis, also known as heartworm or dog heartworm, is a parasitic roundworm that is a type of filarial worm, a small thread-like worm, that causes dirofilariasis. It is spread from host to host through the bites of mosquitoes. There are four genera of mosquitoes that transmit dirofilariasis, Aedes, Culex, Anopheles, and Mansonia.[1] The definitive host is the dog, but it can also infect cats, wolves, coyotes, jackals, foxes, ferrets, bears, seals, sea lions and, under rare circumstances, humans.[2]

"Heartworm" redirects here. For the Whipping Boy album, see Heartworm (album). For the publisher, see Heartworm Press.
"Heartworms" redirects here. For the Shins album, see Heartworms (album).

Dirofilaria immitis
A German Shepherd dog heart infested with Dirofilaria immitis
Scientific classification
Kingdom: Animalia
Phylum: Nematoda
Class: Chromadorea
Order: Rhabditida
Family: Onchocercidae
Genus: Dirofilaria
Species:
D. immitis
Binomial name
Dirofilaria immitis
(Leidy, 1856)

Dirofilaria immitis is commonly called "heartworm". Contrary to their name however, adult heartworms often reside in the pulmonary arterial system (lung arteries) as well as the heart, and a major health effect in the infected animal host is a manifestation of damage to its lung vessels and tissues.[3] In cases involving advanced worm infestation, adult heartworms may migrate to the right heart and the pulmonary artery. Heartworm infection may result in serious complications for the infected host if left untreated, eventually leading to death, most often as a result of secondary congestive heart failure.

Distribution and epidemiology

Although at one time confined to the southern United States, heartworm has now spread to nearly all locations where its mosquito vector is found. In the southeast region of the United States, veterinary clinics saw an average of more than 100 cases of heartworm each in 2016.[4] Transmission of the parasite occurs in all of the United States (cases have even been reported in Alaska), and the warmer regions of Canada. The highest infection rates are found within 150 miles of the coast from Texas to New Jersey, and along the Mississippi River and its major tributaries.[3] It has also been found in South America,[5] southern Europe,[6][7] Southeast Asia,[8] the Middle East,[9] Australia, Korea, and Japan.[10][11]

Course of infection
Lifecycle of D. immitis
Microfilaria (as seen at 400× magnification)

Heartworms go through several life stages before they become adults infecting the pulmonary artery of the host animal. The worms require the mosquito as an intermediate host to complete their lifecycles. The rate of development in the mosquito is temperature-dependent, requiring about two weeks of temperature at or above 27 °C (80 °F). Below a threshold temperature of 14 °C (57 °F), development cannot occur, and the cycle is halted.[12] As a result, transmission is limited to warm weather, and duration of the transmission season varies geographically. The period between the initial infection when the dog is bitten by a mosquito and the maturation of the worms into adults living in the pulmonary arteries takes six to seven months in dogs and is known as the "prepatent period".

The first larval stage (L1) and second larval stage (L2) of heartworm development occurs within the body of a mosquito. Once the larvae develop into the infective third larval stage (L3), the mosquito locates and bites a host, depositing the larvae under the skin at the site of the bite. After a week or two of further growth, they molt into the fourth larval stage (L4) . Then, they migrate to the muscles of the chest and abdomen, and 45 to 60 days after infection, molt to the fifth stage (L5, immature adult). Between 75 and 120 days after infection, these immature heartworms then enter the bloodstream and are carried through the heart to reside in the pulmonary artery. Over the next three to four months, they increase greatly in size. The female adult worm is about 30 cm in length, and the male is about 23 cm, with a coiled tail.[13] By seven months after infection, the adult worms have mated and the females begin giving birth to live young, called microfilariae. Heartworms can live for 5 to 7 years in a dog.[14]

The microfilariae circulate in the bloodstream for as long as two years, and are ingested by bloodsucking mosquitos, where development occurs and the cycle repeats.

Hosts

Hosts of Dirofilaria immitis include:[2]

Reservoir hosts for D. immitis are coyotes and stray dogs.[17]

Clinical signs of infection in dogs

Dogs show no indication of heartworm infection during the six-month prepatent period prior to the worms' maturation, and current diagnostic tests for the presence of microfilariae or antigens cannot detect prepatent infections. Rarely, migrating heartworm larvae get "lost" and end up in aberrant sites, such as the eye, brain, or an artery in the leg, which results in unusual symptoms such as blindness, seizures, and lameness, but normally, until the larvae mature and congregate inside the heart, they produce no symptoms or signs of illness.

Many dogs show little or no sign of infection even after the worms become adults. These animals usually have only a light infection and live a fairly sedentary lifestyle. However, active dogs and those with heavier infections may show the classic signs of heartworm disease. Early signs include a cough, especially during or after exercise, and exercise intolerance. In the most advanced cases where many adult worms have built up in the heart without treatment, signs progress to severe weight loss, fainting, coughing up blood, and finally, congestive heart failure.

There are 4 different classes of symptoms. Class 1, no or mild symptoms with occasional cough. Class 2, mild symptoms with occasional cough and tiredness after moderate activity. Class 3, more severe, sick appearance, persistent cough, tiredness after mild activity. At class 3 trouble breathing and signs of heart failure are common that are seen with a X-ray. Class 4 caval syndrome, blood flow back to the heart is blocked by large mass of worms. only treatment is surgery.[18]

Role of Wolbachia pipientis

Wolbachia pipientis is an intracellular bacterium that is an endosymbiont of D. immitis. All heartworms are thought to be infected with Wolbachia to some degree. The inflammation occurring at the die-off of adult heartworms or larvae is in part due to the release of Wolbachia bacteria or protein into the tissues. This may be particularly significant in cats, in which the disease seems to be more related to larval death than living adult heartworms. Treating heartworm-positive animals with an antibiotic such as doxycycline to remove Wolbachia may prove to be beneficial, but further studies are necessary.[19]

Diagnosis in dogs
Microfilaria of Dirofilaria immitis (heartworms) in a lymph node of a dog with lymphoma. This baby nematode is in a pillow of intermediate-to-large, immature lymphocytes, exhibiting multiple criteria of cancer.[20]

Three methods can be used for the diagnosis:

Microfilarial detection was accomplished most commonly in the past by the microscopic identification of microfilariae on a direct blood smear, above the buffy coat in a microhematocrit tube (or capillary tube), using the modified Knott test, or after millipore filtration. The accuracy of these tests, typically used for routine screening or diagnosis of heartworm infection, is improved by multiple testing. The modified Knott test and millipore filtration are more sensitive because they concentrate microfilariae, improving the chance of diagnosis.[3] The direct smear technique allows examination of larval motion, helping in the distinction of D. immitis from Acanthocheilonema reconditum. This distinction is important because the presence of the latter parasite does not require expensive and potentially harmful therapy. The potential for a microfilaremic infection is 5 - 67%. The number of circulating microfilariae does not correlate with the number of adult heartworms, so is not an indicator of disease severity.[3]

Antigen testing, in most practices, has supplanted or supplemented microfilarial detection. Combining the microfilaria and adult antigen test is most useful in dogs receiving diethylcarbamazine or no preventive (macrolides like ivermectin or moxidectin typically render the dog amicrofilaremic). Up to 1% of infected dogs are microfilaria-positive and antigen-negative.[3]Immunodiagnostics (ELISA, lateral flow immunoassay, rapid immunomigration techniques) to detect heartworm antigen in the host's blood are now regularly used. They can detect occult infections, or infections without the presence of circulating microfilariae. However, these tests are limited in that they only detect the antigens released from the sexually mature female worm's reproductive tract. Therefore, false-negative results may occur during the first five to eight months of infection when the worms are not yet sexually mature.[3] The specificity of these tests is close to 100%, and the sensitivity is more than 80%.[21] A recent study demonstrated a sensitivity of only 64% for infections of only one female worm, but improved with increasing female worm burden (85%, 88%, and 89% for two, three, and four female worms, respectively). Specificity in this study was 97%.[3] False-negative test results can be due to low worm counts, immature infections, and all-male infections.

X-rays are used to evaluate the severity of the heartworm infection and develop a prognosis for the animal. Typically, the changes observed are enlargement of the main pulmonary artery, the right side of the heart, and the pulmonary arteries in the lobes of the lung. Inflammation of the lung tissue is also often observed.[22]

Treatment in dogs

If an animal is diagnosed with heartworms, treatment may be indicated. Before the worms can be treated, however, the dog's heart, liver, and kidney function must be evaluated to determine the risks of treatment. Usually, the adult worms are killed with an arsenic-based compound. The currently approved drug in the US, melarsomine, is marketed under the brand name Immiticide.[23] It has a greater efficacy and fewer side effects than the previously used drug thiacetarsamide, sold as Caparsolate, which makes it a safer alternative for dogs with late-stage infections.

After treatment, the dog must rest, and exercise is to be heavily reduced for several weeks so as to give its body sufficient time to absorb the dead worms without ill effect. Otherwise, if the dog is under exertion, dead worms may break loose and travel to the lungs, potentially causing respiratory failure and sudden death. According to the American Heartworm Society, the administering of aspirin to dogs infected with heartworms is no longer recommended due to a lack of evidence of clinical benefit, and aspirin may be contraindicated in several cases. Aspirin had previously been recommended for its effects on platelet adhesion and the reduction of vascular damage caused by the heartworms.

The course of treatment is not completed until several weeks later, when the microfilariae are dealt with in a separate course of treatment. Once heartworm tests are negative and no surviving worm is detected, the treatment is considered a success, and the patient is effectively cured.

Surgical removal of the adult heartworms as a form of treatment may also be indicated, especially in advanced cases with substantial heart involvement and damage.[24]

Long-term monthly administration of ivermectin year-round at three times the dose normally used for heartworm prevention eventually kills adult heartworms. However, this is not the treatment of choice for removal of adult heartworms for two reasons. Firstly, this treatment is not as effective as melarsomine. More importantly, adult heartworms do not begin to die until 18 months of treatment have elapsed, which is not acceptable for dogs with high-volume infections, and the worms cause irreversible damage to the heart and lungs during that time. Long-term treatment over a year with doxycycline daily and ivermectin/pyrantel has been shown to be effective in early heartworm patients which are asymptomatic.

Prevention of infection in dogs

Prevention of heartworm infection can be obtained through a number of veterinary drugs. The drugs approved for use in the US are ivermectin (sold under the brand names Heartgard, Iverhart, and several other generic versions), milbemycin (Interceptor Flavor Tabs and Sentinel Flavor Tabs) and moxidectin (ProHeart) administered as pills or chewable tablets. Moxidectin is also available in both a six-month and 12-month, sustained-release injection, ProHeart 6 or ProHeart 12, administered by veterinarians. The injectable form of moxidectin was taken off the market in the United States due to safety concerns in 2004, but the FDA returned a newly formulated ProHeart 6 to the market in 2008. ProHeart 6 remains on the market in many other countries, including Canada and Japan. Its sister product, ProHeart 12, is used extensively in Australia and Asia as a 12-month injectable preventive. It was approved for use in the United States by the FDA in July 2019.[25] Topical treatments are available, as well. Advantage Multi (imidacloprid + moxidectin) Topical Solution, uses moxidectin for control and prevention of roundworms, hookworms, heartworms, and whipworms, as well as imidacloprid to kill adult fleas. Selamectin (Revolution) is a topical preventive likewise administered monthly, and can also be used to control fleas, ticks, and mites.

Preventive drugs are highly effective, and when regularly administered, have been shown to protect more than 99% of dogs and cats from heartworm. Most compromises in protection result from the failure to properly administer the drugs during seasonal transmission periods.[26] In regions where the temperature is consistently above 14 °C (57 °F) year-round, a continuous prevention schedule is recommended.

Due to newly emerging resistant strains of heartworms, which no macrocyclic lactone (heartworm prevention) can protect against, the American Heartworm Society recommends dogs be on a repellent and a heartworm preventive. The repellent, such as Vectra 3-D, keeps mosquitoes from feeding on the dog and transmitting the L3 stage worms. If a dog is bitten, the heartworm preventive takes over when administered. If a mosquito feeds on a heartworm positive dog on a repellent, they do not live long enough for the microfilaria they ingested to molt into the infective L3 larva. Vectra 3-D was tested using thousands of mosquitoes infected with the resistant heartworm strain JYD34. In the control group that was given only a placebo, every dog contracted heartworms. In the experimental group that was given only Vectra 3-D, two of eight dogs contracted heartworms and had an average of 1.5 adult worms each. In the experimental group given both heartworm prevention and Vectra 3-D, one dog was infected with L3 stage larvae that did not mature into adulthood due to the heartworm prevention. Using a repellant and a prevention is over 99% effective.[27]

Ivermectin, even with lapses up to four months between doses, still provides 95% protection from adult worms. This period is called the reach-back effect.[28] Since dogs are susceptible to heartworms, they should be tested annually before they start preventive treatment.[17] Annual heartworm testing is highly recommended for pet owners who choose to use minimal dosing schedules. Testing a dog annually for heartworms and other internal parasites is a fundamental part of a complete heartworm prevention program, and is also recommended for dogs who are already on a monthly prevention program.[17]

Heartworm infection in cats

While dogs are a natural host for D. immitis, cats are atypical hosts. Because of this, differences between canine and feline heartworm diseases are significant. The majority of heartworm larvae do not survive in cats, so unlike in dogs, a typical infection in cats is two to five worms. The lifespan of heartworms is considerably shorter in cats, only two to three years, and most infections in cats do not have circulating microfilariae. Cats are also more likely to have aberrant migration of heartworm larvae, resulting in infections in the brain or body cavities.[29]

The infection rate in cats is 1-5% of that in dogs in endemic areas.[30] Both indoor and outdoor cats are infected. The mosquito vector is known to enter homes.[31]

Pathology

The vascular disease in cats that occurs when the L5 larvae invade the pulmonary arteries is more severe than in dogs. A syndrome related to this inflammatory reaction has been identified in cats: heartworm-associated respiratory disease, which can occur three to four months after the initial infection, and is caused by the presence of the L5 larvae in the vessels. The subsequent inflammation of the pulmonary vasculature and lungs can be easily misdiagnosed as feline asthma or allergic bronchitis.[32]

Obstruction of pulmonary arteries due to emboli from dying worms is more likely to be fatal in cats than dogs because of less collateral circulation and fewer vessels.[33] Heartworms can live for 2 to 3 years in cats.[14]

Signs and symptoms

Acute heartworm disease in cats can result in shock, vomiting, diarrhea, fainting, and sudden death. Chronic infection can cause loss of appetite, weight loss, lethargy, exercise intolerance, coughing, and difficulty breathing. The signs of heartworm-associated respiratory disease can persist even after complete elimination of the heartworm infection.[32] Normally, a cat's immune response is strong enough to clear a heartworm infection; however, the immune response itself can cause the same signs associated with heartworm disease.[34] If the infection is resolved, a cat can still have respiratory damage.[34]

Diagnosis

Diagnosis of heartworm infection in cats is problematic. Like in dogs, a positive ELISA test for heartworm antigen is a very strong indication of infection. However, the likelihood of a positive antigen test depends on the number of adult female worms present. If only male worms are present, the test will be negative. Even with female worms, an antigen test usually only becomes positive seven to eight months after infection. Therefore, a cat may have significant clinical signs long before the development of a positive test. Heartworm-associated respiratory disease can be found in cats that never develop adult heartworms and therefore never have a positive antigen test.

An antibody test is also available for feline heartworm infection. It will be positive in the event of exposure to D. immitis, so a cat that has successfully eliminated an infection may still be positive for up to three months. The antibody test is more sensitive than the antigen test, but it does not provide direct evidence of adult infection.[35] It can, however, be considered specific for diagnosing previous larval infections, and therefore fairly specific for heartworm-associated respiratory disease.

X-rays of the chest of a heartworm-infected cat may show an increased width of the pulmonary arteries and focal or diffuse opacities in the lungs. Echocardiography is a fairly sensitive test in cats. Adult heartworms appear as double-lined hyperechoic structures within the heart or pulmonary arteries.[36]

Treatment and prevention
Comparison of probable Dirofilaria repens (left column, from human) and D. immitis (right column, from dog)


Heartworm prevention for cats is available as ivermectin (Heartgard for Cats), milbemycin (Interceptor), or the topical selamectin (Revolution for Cats) and Advantage Multi (imidacloprid + moxidectin) topical solution. Ivermectin, milbemycin, and selamectin are approved for use in cats in the US.

Arsenic compounds have been used for heartworm adulticide treatment in cats, as well as dogs, but seem more likely to cause pulmonary reactions. A significant number of cats develop pulmonary embolisms a few days after treatment. The effects of melarsomine are poorly studied in cats. Due to a lack of studies showing a clear benefit of treatment and the short lifespan of heartworms in cats, adulticide therapy is not recommended, and no drugs are approved in the US for use in cats.[33]

Treatment typically consists of putting the cat on a monthly heartworm preventive and a short-term corticosteroid.[29] Surgery has also been used successfully to remove adult worms. The prognosis for feline heartworm disease is guarded.

Heartworm infection in humans

Dirofilaria are important medical parasites, but diagnosis is unusual and is often only made after an infected person happens to have a chest X-ray following granuloma formation in the lung. The nodule itself may be large enough to resemble lung cancer on the X-ray, and requires a biopsy for a pathologic assessment. This has been shown to be the most significant medical consequence of human infection by the canine heartworm. Patients are infected with the parasite through the bite of an infected mosquito, which is the same mechanism that causes heartworm infection in dogs.[37]

D. immitis is one of many species that can cause infection in dogs and humans. It was thought to infect the human eye, with most cases reported from the southeastern United States. However, these cases are now thought to be caused by a closely related parasite of raccoons, Dirofilaria tenuis. Several hundred cases of subcutaneous infections in humans have been reported in Europe, but these are almost always caused by another closely related parasite, Dirofilaria repens, rather than the dog heartworm. There are proven D. immitis infections,[16] but humans rarely get infected with heartworms due to the larvae never fully maturing.[37] When the heartworm microfilariae migrate through the skin, they often die since heartworms cannot survive in a human host, even if they make it into the bloodstream.[37] Once the heartworms die, the immune system in the human body reacts to their tissue with inflammation as it tries to destroy the heartworms.[37] When this happens, the condition is called pulmonary dirofilariasis.[37] Heartworms in humans is not a serious problem unless they are causing pain, discomfort, and other noticeable symptoms.[37]

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gollark: Yep.

References
  1. Prevention, CDC-Centers for Disease Control and (2019-04-16). "CDC - Dirofliariasis - Biology - Life Cycle of D. immitis". www.cdc.gov. Retrieved 2020-05-07.
  2. "American Heartworm Society | FAQs". Heartwormsociety.org. Retrieved 2014-07-04.
  3. Ettinger, Stephen J.; Feldman, Edward C. (2010). Textbook of Veterinary Internal Medicine (7th ed.). W.B. Saunders Company. ISBN 978-1-4160-6593-7.
  4. Heartworm Incidence Maps. American Heartworm Society. 1970 Apr 10 [cited 2019 Apr 16]. https://www.heartwormsociety.org/pet-owner-resources/incidence-maps Archived 2019-04-23 at the Wayback Machine.
  5. Vezzani D, Carbajo A (2006). "Spatial and temporal transmission risk of Dirofilaria immitis in Argentina". Int J Parasitol. 36 (14): 1463–72. doi:10.1016/j.ijpara.2006.08.012. PMID 17027990.
  6. "Heartworm Disease: Introduction". The Merck Veterinary Manual /. 2006. Retrieved 2007-02-26.
  7. Vieira, Ana Luísa; Vieira, Maria João; Oliveira, João Manuel; Simões, Ana Rita; Diez-Baños, Pablo; Gestal, Juan (2014). "Prevalence of canine heartworm (Dirofilaria immitis) disease in dogs of central Portugal". Parasite. 21: 5. doi:10.1051/parasite/2014003. ISSN 1776-1042. PMC 3927308. PMID 24534524.
  8. Nithiuthai, Suwannee (2003). "Risk of Canine Heartworm Infection in Thailand". Proceedings of the 28th World Congress of the World Small Animal Veterinary Association. Retrieved 2007-02-26.
  9. Rafiee, Mashhady (2005). "Study of Prevalence of Dirofilaria immitis Infestation in Dogs were Examined in Veterinary Clinics of Tabriz Azad University (Iran) during 1992–2002". Proceedings of the 30th World Congress of the World Small Animal Veterinary Association. Retrieved 2007-02-26.
  10. Ettinger, Stephen J.; Feldman, Edward C. (1995). Textbook of Veterinary Internal Medicine (4th ed.). W.B. Saunders Company. ISBN 978-0-7216-6795-9.
  11. Oi, M.; Yoshikawa, S.; Ichikawa, Y.; Nakagaki, K.; Matsumoto, J.; Nogami, S. (2014). "Prevalence of Dirofilaria immitis among shelter dogs in Tokyo, Japan, after a decade: comparison of 1999-2001 and 2009-2011". Parasite. 21: 10. doi:10.1051/parasite/2014008. PMC 3937804. PMID 24581552.
  12. Knight, D. H.; Lok, J. B. (May 1998). "Seasonality of heartworm infection and implications for chemoprophylaxis". Clinical Techniques in Small Animal Practice. 13 (2): 77–82. doi:10.1016/S1096-2867(98)80010-8. ISSN 1096-2867. PMID 9753795.
  13. Johnstone, Colin (1998). "Heartworm". Parasites and Parasitic Diseases of Domestic Animals. University of Pennsylvania. Archived from the original on 2000-12-06. Retrieved 2007-02-26.
  14. "Heartworm Basics - American Heartworm Society". www.heartwormsociety.org. Archived from the original on 2020-05-23. Retrieved 2020-05-07.
  15. Mazzariol, S.; Cassini, R.; Voltan, L.; Aresu, L.; di Regalbono, A. F. (2010). "Heartworm (Dirofilaria immitis) infection in a leopard (Panthera pardus pardus) housed in a zoological park in north-eastern Italy". Parasites & Vectors. 3: 25. doi:10.1186/1756-3305-3-25.
  16. Tumolskaya, Nelli Ignatievna; Pozio, Edoardo; Rakova, Vera Mikhaylovna; Supriaga, Valentina Georgievna; Sergiev, Vladimir Petrovich; Morozov, Evgeny Nikolaevich; Morozova, Lola Farmonovna; Rezza, Giovanni; Litvinov, Serguei Kirillovich (2016). "Dirofilaria immitis in a child from the Russian Federation". Parasite. 23: 37. doi:10.1051/parasite/2016037. ISSN 1776-1042. PMC 5018928. PMID 27600944.
  17. Paul, Mike. 10 Things You Need to Know About Heartworm and Your Dog. Pet Health Network, 10 Sept. 2015 [cited 3 Apr 2019]. www.pethealthnetwork.com/dog-health/dog-diseases-conditions-a-z/10-things-you-need-know-about-heartworm-and-your-dog.
  18. Medicine, Center for Veterinary (2019-08-27). "Keep the Worms Out of Your Pet's Heart! The Facts about Heartworm Disease". FDA.
  19. Todd-Jenkins, Karen (October 2007). "The Role of Wolbachia in heartworm disease". Veterinary Forum. 24 (10): 28–30.
  20. Wheeler, Lance. "Photostream". Flickr. Retrieved 2 December 2017.
  21. Atkins, Clarke (2005). "Heartworm Disease in Dogs: An Update". Proceedings of the 30th World Congress of the World Small Animal Veterinary Association. Retrieved 2007-02-26.
  22. "American Heaartworm Society". Archived from the original on 2013-11-04.
  23. "Product Information". Archived from the original on 2008-08-28. Retrieved 2008-07-01.
  24. "2005 Guidelines For the Diagnosis, Prevention and Management of Heartworm (Dirofilaria immitis) Infection in Dogs". American Heartworm Society. 2005. Archived from the original on 2008-06-16. Retrieved 2008-07-01.
  25. Medicine, Center for Veterinary (2019-07-02). "FDA Approves ProHeart 12 (moxidectin) for Prevention of Heartworm Disease in Dogs". FDA.
  26. Knight, David (1998-05-01). "Heartworm". Seasonality of Heartworm Infection and Implications for Chemoprophylaxis. Topics in Companion Animal Medicine.
  27. "Fight Heartworm Now". Fight Heartworm Now. Archived from the original on 2018-08-18. Retrieved 2018-08-17.
  28. Atwell, R. (1988). "Heartworm". Dirofilariasis (CRC Press, 1988:30–34). Archived from the original on 2011-07-14. Retrieved 2009-05-09.
  29. "2007 Guidelines For the Diagnosis, Treatment and Prevention of Heartworm (Dirofilaria immitis) Infection in Cats". American Heartworm Society. 2007. Archived from the original on 2007-06-14. Retrieved 2007-07-26.
  30. Berdoulay P, Levy JK, Snyder PS, et al. (2004). "Comparison of serological tests for the detection of natural heartworm infection in cats". Journal of the American Animal Hospital Association. 40 (5): 376–84. doi:10.5326/0400376. PMID 15347617.
  31. Atkins CE, DeFrancesco TC, Coats JR, Sidley JA, Keene BW (2000). "Heartworm infection in cats: 50 cases (1985–1997)". J. Am. Vet. Med. Assoc. 217 (3): 355–8. CiteSeerX 10.1.1.204.2733. doi:10.2460/javma.2000.217.355. PMID 10935039.
  32. Yin, Sophia (June 2007). "Update on heartworm infection". Veterinary Forum. 24 (6): 42–43.
  33. Atkins, Clarke E.; Litster, Annette L. (2005). "Heartworm Disease". In August, John R. (ed.). Consultations in Feline Internal Medicine Vol. 5. Elsevier Saunders. ISBN 978-0-7216-0423-7.
  34. Reno, Tabitha. The Myths and Facts of Heartworm Disease in Cats. Whitesburg Animal Hospital, 5 May 2017 [cited 3 Apr 2019]. www.whitesburganimalhospital.com/blog/heartworm-disease-cats/.
  35. Atkins C (1999). "The diagnosis of feline heartworm infection". Journal of the American Animal Hospital Association. 35 (3): 185–7. doi:10.5326/15473317-35-3-185. PMID 10333254.
  36. DeFrancesco TC, Atkins CE, Miller MW, Meurs KM, Keene BW (2001). "Use of echocardiography for the diagnosis of heartworm disease in cats: 43 cases (1985–1997)". J. Am. Vet. Med. Assoc. 218 (1): 66–9. doi:10.2460/javma.2001.218.66. PMID 11149717.
  37. Jewell, Tim. Can Humans Get Heartworms from Dogs? Edited by Daniel Murrell, Healthline, 31 July 2018 [cited 3 Apr 2019]. www.healthline.com/health/heartworms-in-humans.

Further reading
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