Endoglycosylceramidase

In enzymology, an endoglycosylceramidase (EC 3.2.1.123) is an enzyme that catalyzes the chemical reaction

oligoglycosylglucosylceramide + H2O ceramide + oligoglycosylglucose
endoglycosylceramidase
Identifiers
EC number3.2.1.123
CAS number105503-61-5
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO

Thus, the two substrates of this enzyme are oligoglycosylglucosylceramide and H2O, whereas its two products are ceramide and oligoglycosylglucose.

This enzyme belongs to the family of hydrolases, specifically those glycosidases that hydrolyse O- and S-glycosyl compounds. The systematic name of this enzyme class is oligoglycosylglucosylceramide glycohydrolase. Other names in common use include endoglycoceramidase, EGCase, and glycosyl-N-acetyl-sphingosine 1,1-beta-D-glucanohydrolase.

Structural studies

As of late 2007, 6 structures have been solved for this class of enzymes, with PDB accession codes 2OSW, 2OSX, 2OSY, 2OYK, 2OYL, and 2OYM.

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References

    • Ito M, Yamagata T (1986). "A novel glycosphingolipid-degrading enzyme cleaves the linkage between the oligosaccharide and ceramide of neutral and acidic glycosphingolipids". J. Biol. Chem. 261 (30): 14278–82. PMID 3771534.


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