Alpha-neoagaro-oligosaccharide hydrolase

Alpha-neoagaro-oligosaccharide hydrolase (EC 3.2.1.159, alpha-neoagarooligosaccharide hydrolase, alpha-NAOS hydrolase) is an enzyme with systematic name alpha-neoagaro-oligosaccharide 3-glycohydrolase.[1] This enzyme catalyses the following chemical reaction

Hydrolysis of the (1->3)-alpha-L-galactosidic linkages of neoagaro-oligosaccharides that are smaller than a hexamer, yielding 3,6-anhydro-L-galactose and D-galactose
Alpha-neoagaro-oligosaccharide hydrolase
Identifiers
EC number3.2.1.159
CAS number60063-77-6
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum

When neoagarohexaose is used as a substrate, the oligosaccharide is cleaved at the non-reducing end to produce 3,6-anhydro-L-galactose and agaropentaose, which is further hydrolysed to agarobiose and agarotriose.

References

  1. Sugano Y, Kodama H, Terada I, Yamazaki Y, Noma M (November 1994). "Purification and characterization of a novel enzyme, alpha-neoagarooligosaccharide hydrolase (alpha-NAOS hydrolase), from a marine bacterium, Vibrio sp. strain JT0107". Journal of Bacteriology. 176 (22): 6812–8. PMC 197048. PMID 7961439.
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