Lys-N

Lys-N is a metalloendopeptidase found in the mushroom Grifola frondosa that cleaves proteins on the amino side of lysine residues.[1]

Crystal structure of Lys-N with co-ordinated zinc atom.[2]

Mass spectrometry

Lys-N is becoming a popular protease used for protein digestion in proteomics experiments. The combination Lys-N proteolytic peptides and mass spectrometry sequencing with ETD creates tandem mass spectra composed mostly of amino terminal peptide fragment ions.[3] This fragmentation pattern facilitates the applicability of these spectra for de novo peptide sequencing.[3]

gollark: It's still *recognizable*, I'd say.
gollark: I'll see if I can get it to be transparent, but I can get it to downscale at least.
gollark: Do you want me to just half the resolution or something? I can run it through ffmpeg and do that.
gollark: You can't really shrink that GIF further without losing quality in some way, if you want it to remain a GIF.
gollark: Well, if you convert it to a video then the video itself is smaller, because video codecs/formats are... actually optimized for videos; GIFs are not.

See also

References
  1. Nonaka T, Hashimoto Y, Takio K (July 1998). "Kinetic characterization of lysine-specific metalloendopeptidases from Grifola frondosa and Pleurotus ostreatus fruiting bodies". Journal of Biochemistry. 124 (1): 157–62. doi:10.1093/oxfordjournals.jbchem.a022074. PMID 9644258.
  2. RCSB Protein Data Bank - RCSB PDB - 1G12 Structure Summary
  3. Taouatas N, Drugan MM, Heck AJ, Mohammed S (May 2008). "Straightforward ladder sequencing of peptides using a Lys-N metalloendopeptidase". Nature Methods. 5 (5): 405–7. doi:10.1038/nmeth.1204. PMID 18425140.
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