Cysteine—tRNA ligase
In enzymology, a cysteine-tRNA ligase (EC 6.1.1.16) is an enzyme that catalyzes the chemical reaction
- ATP + L-cysteine + tRNACys AMP + diphosphate + L-cysteinyl-tRNACys
| cysteine-tRNA ligase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 6.1.1.16 | ||||||||
| CAS number | 37318-56-2 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
The 3 substrates of this enzyme are ATP, L-cysteine, and tRNA(Cys), whereas its 3 products are AMP, diphosphate, and L-cysteinyl-tRNA(Cys).
This enzyme belongs to the family of ligases, to be specific those forming carbon-oxygen bonds in aminoacyl-tRNA and related compounds. The systematic name of this enzyme class is L-cysteine:tRNACys ligase (AMP-forming). Other names in common use include cysteinyl-tRNA synthetase, cysteinyl-transferRNA synthetase, cysteinyl-transfer ribonucleate synthetase, and cysteine translase. This enzyme participates in cysteine metabolism and aminoacyl-trna biosynthesis.
Structural studies
As of late 2007, 3 structures have been solved for this class of enzymes, with PDB accession codes 1LI5, 1LI7, and 1U0B.
References
- Mccorquodale DJ (December 1964). "The separation and partial purification of aminoacyl-RNA synthetases from Escherichia coliI". Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects. 91 (4): 541–8. doi:10.1016/0926-6550(64)90001-5. PMID 14262440.