Cyanophycinase
Cyanophycinase (EC 3.4.15.6, cyanophycin degrading enzyme, beta-Asp-Arg hydrolysing enzyme, CGPase, CphB, CphE, cyanophycin granule polypeptidase, extracellular CGPase) is an enzyme.[1][2][3] It catalyses the following chemical reaction
- [L-Asp(4-L-Arg)]n + H2O [L-Asp(4-L-Arg)]n-1 + L-Asp(4-L-Arg)
| Cyanophycinase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 3.4.15.6 | ||||||||
| CAS number | 131554-16-0 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
| Cyanophycinase | |||||||
|---|---|---|---|---|---|---|---|
| Identifiers | |||||||
| Organism | |||||||
| Symbol | cphB | ||||||
| PDB | 3EN0 | ||||||
| UniProt | P73832 | ||||||
| |||||||
The enzyme is highly specific for the branched polypeptide cyanophycin. It is similar to Dipeptidase E, another S51 family serine protease.
References
- Obst M, Krug A, Luftmann H, Steinbüchel A (July 2005). "Degradation of cyanophycin by Sedimentibacter hongkongensis strain KI and Citrobacter amalonaticus strain G Isolated from an anaerobic bacterial consortium". Applied and Environmental Microbiology. 71 (7): 3642–52. doi:10.1128/aem.71.7.3642-3652.2005. PMC 1169039. PMID 16000772.
- Obst M, Oppermann-Sanio FB, Luftmann H, Steinbüchel A (July 2002). "Isolation of cyanophycin-degrading bacteria, cloning and characterization of an extracellular cyanophycinase gene (cphE) from Pseudomonas anguilliseptica strain BI. The cphE gene from P. anguilliseptica BI encodes a cyanophycinhydrolyzing enzyme". The Journal of Biological Chemistry. 277 (28): 25096–105. doi:10.1074/jbc.m112267200. PMID 11986309.
- Richter R, Hejazi M, Kraft R, Ziegler K, Lockau W (July 1999). "Cyanophycinase, a peptidase degrading the cyanobacterial reserve material multi-L-arginyl-poly-L-aspartic acid (cyanophycin): molecular cloning of the gene of Synechocystis sp. PCC 6803, expression in Escherichia coli, and biochemical characterization of the purified enzyme". European Journal of Biochemistry. 263 (1): 163–9. doi:10.1046/j.1432-1327.1999.00479.x. PMID 10429200.
External links
- Cyanophycinase at the US National Library of Medicine Medical Subject Headings (MeSH)
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