Ampoule

An ampoule (also ampul and ampule) is a small sealed vial which is used to contain and preserve a sample, usually a solid or liquid. Ampoules are made of glass.

Ampoules containing pharmaceutical products

A large ampoule containing 1.4 kg of high purity caesium.

Modern ampoules are most commonly used to contain pharmaceuticals and chemicals that must be protected from air and contaminants. They are hermetically sealed by melting the thin top with an open flame, and usually opened by snapping off the neck. The space above the chemical may be filled with an inert gas before sealing. The walls of glass ampoules are usually sufficiently strong to be brought into a glovebox without any difficulty.

Glass ampoules are more expensive than bottles and other simple containers, but there are many situations where their superior imperviousness to gases and liquids and all-glass interior surface are worth the extra cost. Examples of chemicals sold in ampoules are injectable pharmaceuticals, air-sensitive reagents like tetrakis(triphenylphosphine)palladium(0), hygroscopic materials like deuterated solvents and trifluoromethanesulfonic acid, and analytical standards.

Ampoules can be pressurised, have air evacuated from them, and have the air in the ampoule replaced with other gasses, often inert ones. The radio-pharmaceutical Xenon-133 often is packaged in glass ampoules[1] and specially-shaped glass ampoules have long been used for samples of gaseous elements, such as all of the Column 18 Noble Gasses save radon (mainly because it is radioactive with a half life less than half a week) and special thick-walled quartz and fluorite ampoules under high pressure containing fluorine and chlorine liquified by the high pressure[2]

Teflon ampoules have been developed, based on the concept of the Teflon jug for high-molarity hydrofluoric acid,[3] for containing chemicals that would corrode and/or ignite glass and/or contaminate themselves, corrode, or disintegrate metal containers where the reagent does not passivate the metal by rapidly forming a layer of a new inert compound on the metal surface reliably and predictably or at all.

Photosensitive chemicals like many 14-dihydromorphinone opioids like hydromorphone and oxymorphone, various silver salts and so on can be packaged in ampoules of smoked glass, glass with chemicals added in manufacturing that filter out ultraviolet and other types of light, or be made with an opaque top and bottom (usually painted with opaque paint) and the rest of the ampoule wrapped in thick paper.

Historic ampoules

A collection of ancient ampoules

Historically, ampoules were used to contain a small sample of a person's blood after death, which was entombed alongside them in many Christian catacombs. It was originally believed that only martyrs were given this burial treatment, though it is suspected to have been a widely practiced tradition.[4]

San Gennaro

An ampoule, allegedly dating back to the year 305 and filled with the blood of Saint Januarius (San Gennaro), bishop of Benevento, has been kept for centuries in the Cathedral at Naples. Every year on 19 September the town celebrates the Feast of San Gennaro, when the solid reddish-brown contents of the ampoule usually liquifies after being taken out of a safe, carried in procession and placed on the Cathedral's altar.[5]

Sainte Ampoule

The Holy Ghost brings the Holy Ampulla for the baptism of Clovis I

Another well-known ampoule is the Holy Ampulla (Sainte Ampoule) which held the anointing oil for the coronation of the French monarchs. The oil was allegedly passed down from the time of Clovis I; it was kept for a time in the tomb of Saint Remigius and later in the Cathedral of Notre-Dame, Reims. It was used in coronation of Charles X.

Production and usage

Fabrication

Modern glass ampoules are produced industrially from short lengths of glass tubing, shaped by heating with gas torches and gravity in automated production lines. Computer vision techniques are usually employed for quality control.

Ampoule codes

Diagram of an ampoule showing color-coded neck rings.

Ampoules often have colored rings of paint or enamel around their necks that are meant to identify the substance inside the ampoule. The rings are machine-readable and allow for accurate handling of the substance for the purposes of storage, labeling, and secondary packaging.[6]

Color coding of modern ampoules is done during the manufacturing process. A machine applies colored rings on the ampoule between the two ovens. [6]

Filling

The filling and sealing of ampoules may be done by automated machinery on an industrial scale, or by hand in small-scale industries and laboratory. Ampoule filling machines can be categorized in three categories called automatic machine, semi automatic machine and manual (hand-operated) machines.

Blank ampoules can be purchased from scientific glass supply houses and sealed with a small gas torch.

A Schlenk line may be used for sealing under inert atmospheres. There is procedure of purging nitrogen before and after filling liquid into ampoules in order to remove atmospheric air available inside the ampoules.

Opening

Ampoules are opened by scoring the neck and snapping the top off. In the "one point cut" (OPC ampoules) a dot above the neck identifies the location of a small cut in the glass to help breaking/opening the ampoule.

If properly done, this last operation creates a clean break without any extra glass shards or slivers; but the liquid or solution may be filtered for greater assurance.

Contamination concerns

Glass particle contamination is of ongoing concern, with patients who receive medication parenterally, such as intravenously under hospital care, at greater risk of receiving glass particulates when medication is aspirated.[7] A 2016 study of 180 ampoules found 19,473 glass particles in aspirated fluids, with filtering only reducing the mean from 114 to 89 particles per ampoule. Glass particle contamination of an ampule occurred with all intravenous injection methods.[8]

Other uses

Ampoules are common practice as containers of low frequency RFID tags. These are used mainly for tagging animals for identification.[9]

See also

• Ampulla

References

• Hans-Jürgen Bässler und Frank Lehmann : Containment Technology: Progress in the Pharmaceutical and Food Processing Industry. Springer, Berlin 2013, ISBN 978-3642392917

1. Veal, N. (1 June 1965). "The handling and dispensing of xenon-133: Gas shipments for clinical use". The International Journal of Applied Radiation and Isotopes. 16 (6): 385–387. doi:10.1016/0020-708X(65)90023-2. PMID 14327645.
2. "Pressurized ampule, a sample of the element Chlorine in the Periodic Table".
3. "The acid that really does eat through everything". the chronicle flask. 16 April 2013.
4. (in Italian) Seroux d'Agincourt, Viaggio nelle catacombe di Roma di un membro dell'Accademia di Cortona (1810), pp. 200-1, Milano, G. Silvestri, 1835.
5. Thurston, Herbert (1910). "Saint Januarius". Catholic Encyclopedia.
6. "Pharmaceutical Packaging Machinery for Pharma | Bausch+Ströbel Germany". Archived from the original on 12 March 2013. Retrieved 14 February 2013.
7. Lye, S. T.; Hwang, N. C. (January 2003). "Glass particle contamination: is it here to stay?". Anaesthesia. 58 (1): 93–94. doi:10.1046/j.1365-2044.2003.296812.x. PMID 12492680.
8. Joo, Ga Eul; Sohng, Kyeong-Yae; Park, Michael Yong (6 January 2016). "The effect of different methods of intravenous injection on glass particle contamination from ampules". SpringerPlus. 5: 15. doi:10.1186/s40064-015-1632-0. PMC 4703599. PMID 26759754.
9. Wassmer, Thomas; Murray, Dennis; Boutin, Stan; Fahlman, Andreas; Jensen, Frants Havmand, eds. (2020). Ecology and Behaviour of Free-Ranging Animals Studied by Advanced Data-Logging and Tracking Techniques. Frontiers Media SA. p. 216. doi:10.3389/978-2-88963-792-8. ISBN 978-2889637928.