Levansucrase

Levansucrase (EC 2.4.1.10) is an enzyme that catalyzes the chemical reaction

sucrose + (2,6-beta-D-fructosyl)n glucose + (2,6-beta-D-fructosyl)n+1
levansucrase
Levansucrase monomer, Bacillus subtilis
Identifiers
EC number2.4.1.10
CAS number9030-17-5
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO

Thus, the two substrates of this enzyme are sucrose and (2,6-beta-D-fructosyl)n, whereas its two products are glucose and (2,6-beta-D-fructosyl)n+1.

This enzyme belongs to the family of glycosyltransferases, specifically the hexosyltransferases. The systematic name of this enzyme class is sucrose:2,6-beta-D-fructan 6-beta-D-fructosyltransferase. Other names in common use include sucrose 6-fructosyltransferase, beta-2,6-fructosyltransferase, and beta-2,6-fructan:D-glucose 1-fructosyltransferase. This enzyme participates in starch and sucrose metabolism and two-component system - general.

Structural studies

As of late 2007, 3 structures have been solved for this class of enzymes, with PDB accession codes 1OYG, 1PT2, and 1W18.

gollark: There's "only" about 541 lines of code here, I'm sure I can come up with a better implementation again.
gollark: OH APIOFORMS, my webcrawler code was SO VAGUELY BAD.
gollark: Yes, that is the shadily appropriate way.
gollark: RFS works because you have a human there to interpret it very freeformly.
gollark: That wouldn't really work.

References
    • Hehre EJ (1951). "Enzymic synthesis of polysaccharides: a biological type of polymerization". Adv. Enzymol. Relat. Subj. Biochem. Advances in Enzymology - and Related Areas of Molecular Biology. 11: 297–337. doi:10.1002/9780470122563.ch6. ISBN 978-0-470-12256-3.
    • AVIGAD G, FEINGOLD DS, HESTRIN S (1956). "The mechanism of polysaccharide production from sucrose. 3 Donor-acceptor specificity of levansucrase from Aerobacter levanicum". Biochem. J. 64 (2): 340–51. PMC 1199737. PMID 13363847.
    • Reese ET, Avigad G (1966). "Purification of levansucrase by precipitation with levan". Biochim. Biophys. Acta. 113 (1): 79–83. doi:10.1016/s0926-6593(66)80123-6. PMID 5940635.


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