Dolichyl-diphosphooligosaccharide–protein glycotransferase
In enzymology, a dolichyl-diphosphooligosaccharide–protein glycotransferase (EC 2.4.99.18) is an enzyme that catalyzes the chemical reaction
- dolichyl diphosphooligosaccharide + protein L-asparagine dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine
| dolichyl-diphosphooligosaccharide-protein glycotransferase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 2.4.99.18 | ||||||||
| CAS number | 75302-32-8 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
| Oligosaccaryltransferase | |
|---|---|
| Identifiers | |
| Symbol | ? |
| InterPro | IPR018943 |
| OPM superfamily | 242 |
| OPM protein | 2lat |
| Membranome | 275 |
Thus, the two substrates of this enzyme are dolichyl diphosphooligosaccharide and protein L-asparagine, whereas its 3 products are dolichyl diphosphate, glycoprotein with the oligosaccharide chain attached by N-glycosyl, and linkage to protein L-asparagine.
This enzyme belongs to the family of glycosyltransferases, specifically the hexosyltransferases. The systematic name of this enzyme class is dolichyl-diphosphooligosaccharide:protein-L-asparagine oligopolysaccharidotransferase. Other names in common use include dolichyldiphosphooligosaccharide-protein glycosyltransferase, asparagine N-glycosyltransferase, dolichyldiphosphooligosaccharide-protein oligosaccharyltransferase, dolichylpyrophosphodiacetylchitobiose-protein glycosyltransferase, oligomannosyltransferase, oligosaccharide transferase, dolichyldiphosphoryloligosaccharide-protein, and oligosaccharyltransferase. This enzyme participates in n-glycan biosynthesis and glycan structures - biosynthesis 1.
References
- Das RC, Heath EC (1980). "Dolichyldiphosphoryloligosaccharide--protein oligosaccharyltransferase; solubilization, purification, and properties". Proc. Natl. Acad. Sci. U.S.A. 77 (7): 3811–5. doi:10.1073/pnas.77.7.3811. PMC 349716. PMID 6933437.