Cob(I)yrinic acid a,c-diamide adenosyltransferase

In molecular biology, cob(I)yrinic acid a,c-diamide adenosyltransferase (also known as ATP:cob(I)alamin adenosyltransferase or ATP:corrinoid adenosyltransferase) EC 2.5.1.17 is an enzyme which catalyses the conversion of cobalamin (vitamin B12) into one of its coenzyme forms, adenosylcobalamin (coenzyme B12, AdoCbl).[2][3] Adenosylcobalamin is required as a cofactor for the activity of certain enzymes. AdoCbl contains an adenosyl moiety liganded to the cobalt ion of cobalamin via a covalent Co-C bond.

ATP:corrinoid adenosyltransferase
Human mitochondrial Cob(I)yrinic acid a,c-diamide adenosyltransferase, MMAB.[1]
Identifiers
EC number2.5.1.17
CAS number37277-84-2
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO
ATP:corrinoid adenosyltransferase
the three-dimensional structure of atp:corrinoid adenosyltransferase from salmonella typhimurium. apo-atp form
Identifiers
SymbolCobA_CobO_BtuR
PfamPF02572
Pfam clanCL0023
InterProIPR003724
SCOPe1g64 / SUPFAM
Cobalamin adenosyltransferase (PduO/EutT)
crystal structure of conserved protein 0546 from thermoplasma acidophilum
Identifiers
SymbolCob_adeno_trans
PfamPF01923
InterProIPR002779
SCOPe1nog / SUPFAM

ATP:cob(I)alamin adenosyltransferases are classed into three groups: CobA-type,[4] EutT-type [5] and PduO-type.[6] Each of the three enzyme types appears to be specialised for particular AdoCbl-dependent enzymes or for the de novo synthesis of AdoCbl. PduO and EutT are distantly related, sharing short conserved motifs, while CobA is evolutionarily unrelated and is an example of convergent evolution.

The CobA group includes the ATP:cob(I)alamin adenosyltransferases CobA (Salmonella typhimurium), CobO (Pseudomonas denitrificans), and ButR (Escherichia coli). There is a high degree of sequence identity between these proteins.[7] CobA is responsible for attaching the adenosyl moiety from ATP to the cobalt ion of the corrin ring, necessary for the conversion of cobalamin to adenosylcobalamin.[3][4] PduO functions to convert cobalamin to AdoCbl for 1,2-propanediol degradation,[8] while EutT produces AdoCbl for ethanolamine utilisation.[9]

Synonyms

This enzyme is also known as:

  • Cobalamin adenosyltransferase
  • ATP:cob(I)alamin adenosyltransferase
  • ATP:corrinoid adenosyltransferase
gollark: At least it's better than a government just throwing money at the system to try and give everyone a degree they might not actually *need* in a sensible market which didn't discriminate that way.
gollark: <@!330678593904443393> Somewhat, sure!
gollark: My problem with the whole free-college/university thing (again, see here: https://slatestarcodex.com/2015/06/06/against-tulip-subsidies/) is that it's just propping up what seems to basically just be an expensive and time-consuming signalling scheme at great cost.
gollark: Frequently.
gollark: Er, I was talking about university/college being a nigh-pointless signalling thing.

See also

References
  1. Schubert HL, Hill CP (December 2006). "Structure of ATP-bound human ATP:cobalamin adenosyltransferase". Biochemistry. 45 (51): 15188–96. doi:10.1021/bi061396f. PMC 2532598. PMID 17176040.
  2. Mera PE, Escalante-Semerena JC (September 2010). "Multiple roles of ATP:cob(I)alamin adenosyltransferases in the conversion of B12 to coenzyme B12". Appl. Microbiol. Biotechnol. 88 (1): 41–8. doi:10.1007/s00253-010-2773-2. PMC 3034633. PMID 20677021.
  3. Sheppard DE, Penrod JT, Bobik T, Kofoid E, Roth JR (November 2004). "Evidence that a B12-adenosyl transferase is encoded within the ethanolamine operon of Salmonella enterica". J. Bacteriol. 186 (22): 7635–44. doi:10.1128/JB.186.22.7635-7644.2004. PMC 524904. PMID 15516577.
  4. Buan NR, Rehfeld K, Escalante-Semerena JC (May 2006). "Studies of the CobA-type ATP:Co(I)rrinoid adenosyltransferase enzyme of Methanosarcina mazei strain Go1". J. Bacteriol. 188 (10): 3543–50. doi:10.1128/JB.188.10.3543-3550.2006. PMC 1482872. PMID 16672609.
  5. Buan NR, Suh SJ, Escalante-Semerena JC (September 2004). "The eutT gene of Salmonella enterica Encodes an oxygen-labile, metal-containing ATP:corrinoid adenosyltransferase enzyme". J. Bacteriol. 186 (17): 5708–14. doi:10.1128/JB.186.17.5708-5714.2004. PMC 516830. PMID 15317775.
  6. Johnson CL, Pechonick E, Park SD, Havemann GD, Leal NA, Bobik TA (March 2001). "Functional genomic, biochemical, and genetic characterization of the Salmonella pduO gene, an ATP:cob(I)alamin adenosyltransferase gene". J. Bacteriol. 183 (5): 1577–84. doi:10.1128/JB.183.5.1577-1584.2001. PMC 95042. PMID 11160088.
  7. Suh SJ, Escalante-Semerena JC (July 1993). "Cloning, sequencing and overexpression of cobA which encodes ATP:corrinoid adenosyltransferase in Salmonella typhimurium". Gene. 129 (1): 93–7. doi:10.1016/0378-1119(93)90701-4. PMID 7916712.
  8. Luers F, Seyfried M, Daniel R, Gottschalk G (September 1997). "Glycerol conversion to 1,3-propanediol by Clostridium pasteurianum: cloning and expression of the gene encoding 1,3-propanediol dehydrogenase". FEMS Microbiol. Lett. 154 (2): 337–45. doi:10.1016/s0378-1097(97)00351-0. PMID 9311132.
  9. Buan NR, Escalante-Semerena JC (June 2006). "Purification and initial biochemical characterization of ATP:Cob(I)alamin adenosyltransferase (EutT) enzyme of Salmonella enterica". J. Biol. Chem. 281 (25): 16971–7. doi:10.1074/jbc.M603069200. PMID 16636051.
This article incorporates text from the public domain Pfam and InterPro: IPR003724
This article incorporates text from the public domain Pfam and InterPro: IPR002779
This article is issued from Wikipedia. The text is licensed under Creative Commons - Attribution - Sharealike. Additional terms may apply for the media files.