6G-fructosyltransferase
In enzymology, a 6G-fructosyltransferase (EC 2.4.1.243) is an enzyme that catalyzes the chemical reaction
- [1-beta-D-fructofuranosyl-(2->1)-]m+1 alpha-D-glucopyranoside + [1-beta-D-fructofuranosyl-(2->1)-]n+1 alpha-D-glucopyranoside [1-beta-D-fructofuranosyl-(2->1)-]m alpha-D-glucopyranoside + [1-beta-D-fructofuranosyl-(2->1)-]n+1 beta-D-fructofuranosyl-(2->6)-alpha-D-glucopyranoside (m > 0; n > 0)
| 6G-fructosyltransferase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 2.4.1.243 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
Thus, the two substrates of this enzyme are [[[1-beta-D-fructofuranosyl-(2->1)-]m+1 alpha-D-glucopyranoside]] and [[[1-beta-D-fructofuranosyl-(2->1)-]n+1 alpha-D-glucopyranoside]], whereas its 4 products are [[[1-beta-D-fructofuranosyl-(2->1)-]m alpha-D-glucopyranoside]], [[[1-beta-D-fructofuranosyl-(2->1)-]n+1]], [[beta-D-fructofuranosyl-(2->6)-alpha-D-glucopyranoside (m > 0; n >=]], and 0.
This enzyme belongs to the family of glycosyltransferases, specifically the hexosyltransferases. The systematic name of this enzyme class is 1F-oligo[beta-D-fructofuranosyl-(2->1)-]sucrose 6G-beta-D-fructotransferase. Other names in common use include fructan:fructan 6G-fructosyltransferase, 1F(1-beta-D-fructofuranosyl)m, sucrose:1F(1-beta-D-fructofuranosyl)nsucrose, 6G-fructosyltransferase, 6G-FFT, 6G-FT, and 6G-fructotransferase.
References
- Shiomi N (1981). "Purification and characterisation of 6G-fructosyltransferase from the roots of asparagus (Asparagus officinalis L.)". Carbohydr. Res. 96 (2): 281–292. doi:10.1016/S0008-6215(00)81878-9.
- Shiomi N (1982). "Reverse reaction of fructosyl transfer catalysed by asparagus 6G-fructosyltransferase". Carbohydr. Res. 106: 166–169. doi:10.1016/S0008-6215(00)80743-0.
- Shiomi N, Ueno K (2004). "Cloning and expression of genes encoding fructosyltransferases from higher plants in food technology". J. Appl. Glycosci. 51 (2): 177–183. doi:10.5458/jag.51.177.
- Ueno K, Onodera S, Kawakami A, Yoshida M, Shiomi N (March 2005). "Molecular characterization and expression of a cDNA encoding fructan:fructan 6G-fructosyltransferase from asparagus (Asparagus officinalis)". The New Phytologist. 165 (3): 813–24. doi:10.1111/j.1469-8137.2004.01294.x. PMID 15720693.