UCHL3

Ubiquitin carboxyl-terminal hydrolase isozyme L3 is an enzyme that in humans is encoded by the UCHL3 gene.[5][6]

UCHL3
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1UCH, 1XD3
Identifiers
Aliases	UCHL3, UCH-L3, ubiquitin C-terminal hydrolase L3
External IDs	OMIM: 603090 MGI: 1355274 HomoloGene: 4377 GeneCards: UCHL3
Gene location (Human) Chr. Chromosome 13 (human)[1] Band 13q22.2 Start 75,549,480 bp[1] End 75,606,020 bp[1]
Gene location (Mouse) Chr. Chromosome 14 (mouse)[2] Band 14 E2.3|14 50.9 cM Start 101,653,967 bp[2] End 101,696,125 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • GO:0001948 protein binding • cysteine-type peptidase activity • hydrolase activity • peptidase activity • ubiquitin binding • NEDD8-specific protease activity • thiol-dependent ubiquitinyl hydrolase activity • thiol-dependent ubiquitin-specific protease activity Cellular component • cell nucleus • intracellular • cytoplasm • cytosol Biological process • protein catabolic process • proteolysis • ubiquitin-dependent protein catabolic process • post-translational modification • protein deubiquitination • protein ubiquitination Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	7347	50933
Ensembl	ENSG00000118939	ENSMUSG00000022111
UniProt	P15374	Q9JKB1
RefSeq (mRNA)	NM_001270952 NM_006002	NM_016723
RefSeq (protein)	NP_001257881 NP_005993	NP_057932
Location (UCSC)	Chr 13: 75.55 – 75.61 Mb	Chr 14: 101.65 – 101.7 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Interactions

UCHL3 has been shown to interact with NEDD8 and the tauopathy and synucleinopathy associated mutated ubiquitin molecule UBB+1.[7][8]

See also

• Ubiquitin carboxy-terminal hydrolase L1—an enzyme

References

1. GRCh38: Ensembl release 89: ENSG00000118939 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000022111 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Wilkinson KD, Lee KM, Deshpande S, Duerksen-Hughes P, Boss JM, Pohl J (Dec 1989). "The neuron-specific protein PGP 9.5 is a ubiquitin carboxyl-terminal hydrolase". Science. 246 (4930): 670–3. doi:10.1126/science.2530630. PMID 2530630.
6. "Entrez Gene: UCHL3 ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)".
7. Dennissen FJ, Kholod N, Hermes DJ, Kemmerling N, Steinbusch HW, Dantuma NP, van Leeuwen FW (July 2011). "Mutant ubiquitin (UBB(+1)) associated with neurodegenerative disorders is hydrolyzed by ubiquitin C-terminal hydrolase L3 (UCH-L3)". FEBS Lett. 585 (16): 2568–74. doi:10.1016/j.febslet.2011.06.037. PMID 21762696.
8. Wada H, Kito K, Caskey LS, Yeh ET, Kamitani T (October 1998). "Cleavage of the C-terminus of NEDD8 by UCH-L3". Biochem. Biophys. Res. Commun. 251 (3): 688–92. doi:10.1006/bbrc.1998.9532. PMID 9790970.

Further reading

• Johnston SC, Larsen CN, Cook WJ, et al. (1997). "Crystal structure of a deubiquitinating enzyme (human UCH-L3) at 1.8 A resolution". EMBO J. 16 (13): 3787–96. doi:10.1093/emboj/16.13.3787. PMC 1170002. PMID 9233788.
• Wilkinson KD, Laleli-Sahin E, Urbauer J, et al. (1999). "The binding site for UCH-L3 on ubiquitin: mutagenesis and NMR studies on the complex between ubiquitin and UCH-L3". J. Mol. Biol. 291 (5): 1067–77. doi:10.1006/jmbi.1999.3038. PMID 10518943.
• Baek SH, Yoo YJ, Tanaka K, Chung CH (1999). "Molecular cloning of chick UCH-6 which shares high similarity with human UCH-L3: its unusual substrate specificity and tissue distribution". Biochem. Biophys. Res. Commun. 264 (1): 235–40. doi:10.1006/bbrc.1999.1492. PMID 10527871.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Saito S, Iida A, Sekine A, et al. (2003). "Catalog of 680 variations among eight cytochrome p450 ( CYP) genes, nine esterase genes, and two other genes in the Japanese population". J. Hum. Genet. 48 (5): 249–70. doi:10.1007/s10038-003-0021-7. PMID 12721789.
• Nam MJ, Madoz-Gurpide J, Wang H, et al. (2004). "Molecular profiling of the immune response in colon cancer using protein microarrays: occurrence of autoantibodies to ubiquitin C-terminal hydrolase L3". Proteomics. 3 (11): 2108–15. doi:10.1002/pmic.200300594. PMID 14595809.
• Dunham A, Matthews LH, Burton J, et al. (2004). "The DNA sequence and analysis of human chromosome 13". Nature. 428 (6982): 522–8. doi:10.1038/nature02379. PMC 2665288. PMID 15057823.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Misaghi S, Galardy PJ, Meester WJ, et al. (2005). "Structure of the ubiquitin hydrolase UCH-L3 complexed with a suicide substrate". J. Biol. Chem. 280 (2): 1512–20. doi:10.1074/jbc.M410770200. PMID 15531586.
• Rolén U, Kobzeva V, Gasparjan N, et al. (2006). "Activity profiling of deubiquitinating enzymes in cervical carcinoma biopsies and cell lines". Mol. Carcinog. 45 (4): 260–9. doi:10.1002/mc.20177. PMID 16402389.
• Lim J, Hao T, Shaw C, et al. (2006). "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration". Cell. 125 (4): 801–14. doi:10.1016/j.cell.2006.03.032. PMID 16713569.