Slit-Robo

Slit-Robo is the name of a cell signaling pathway with many diverse functions including axon guidance and angiogenesis.

Slit refers to a secreted protein that is most widely known as a repulsive axon guidance cue, and Robo refers to its transmembrane protein receptor. There are four different Robos and three Slits in vertebrates: Robo1, Robo2, Robo3/Rig-1, and Robo4, and Slit1, Slit2, Slit3.[1] There are three Robos and a single Slit in Drosophila. The corresponding Slit and Robo homologues in C. elegans are Slt and Sax-3, respectively.[2]

Slits are characterized by four distinct domains, each containing variable numbers of leucine-rich repeats (LRRs),[3] seven to nine EGF repeats,[4][5] an ALPS domain (Agrin, Perlecan, Laminin, Slit), and a cysteine knot.[6] Robos are characterized by five Ig-like domains, three fibronectin type III (FNIII) repeats, a transmembrane portion, and an intracellular tail with up to four conserved cytoplasmic motifs: CC0 (a potential site of tyrosine phosphorylation),[7] CC1 (also a potential site of tyrosine phosphorylation and binds P3 domain of netrin-1 receptor DCC),[8] CC2 (polyproline stretch; consensus binding site for Ena/Vasp proteins),[7] and CC3 (polyproline stretch).[9]

Background and discovery

In the developing nervous system of bilaterians, most axons cross over to the opposite (contralateral) side of the body. What are the genes that ensure that this process occurs appropriately? This fundamental question in axon guidance led researchers to Robo, which was identified in a large-scale screening of Drosophila mutants in the early 1990s.[10] Robo expression was shown to be required for repulsion of axons from the midline, both in ipsilateral axons that never cross the midline and in commissural axons that had already crossed.[9] Another protein Commissureless (Comm) was found to be an essential regulator of Robo: in comm mutants, Robo activity is too high, and no axons cross the midline.[11] Several years later, genetic evidence,[12] biochemical binding experiments, and explant assays[13] identified Slits as the repulsive ligands for Robo receptors in both Drosophila and vertebrates. Slit was also found to act as a repulsive cue in olfactory bulb guidance.[14][15] The high conservation of Slit and Robo structures [16] and the similarities in their function among vertebrates and invertebrates[17] make a strong case for an evolutionarily conserved requirement for Slit/Robo signaling in the developing nervous system.

Cell signaling pathways

Slit-robo binding

The functional region of Slit proteins is located within the leucine-rich repeats (LRRs).[18][19] Slit2 binds Robo1 in a flexible linkage between its D2 domain and the first two Ig-like domains of Robo1.[20] Research suggests that heparan sulfate proteoglycans, which are required for Slit signaling in Drosophila,[21] may support this interaction through stabilization of the Slit-Robo complex or by acting as co-receptors that present Slits to Robos.[22]

Intracellular robo-binding events

Function of Slit-Robo signaling is influenced by binding of intracellular factors to the cytoplasmic domains of Robo.

Abelson and Enabled

In Drosophila, the two proteins Abelson tyrosine kinase (Abl) and Enabled (Ena) mediate cytoskeletal remodeling downstream of Slit-Robo signaling. Abl can phosphorylate Robo's CC0 and CC1 domains thereby down-regulating Robo activity, while Ena interacts with CC0 and CC2 to mediate repulsive signaling.[7] Abl is also thought to promote repulsive signaling by binding to adenylyl cyclase associated proteins (CAP), which regulate actin polymerization.[23]

Rho GTPases

Binding of Slit to Robo induces binding of SrGAP1 to the CC3 domain of Robo1, which leads to downstream deactivation of Cdc42, a Rho GTPase which mediates actin polymerization, and activation of RhoA, a Rho GTPase which mediates actin depolymerization.[24] In Drosophila, the SH3-SH2 adaptor protein Dock binds directly to the CC2 and CC3 domains of Robo, recruiting p21-activated protein kinase (Pak) and Sos, resulting in increased Rac activity. This Robo-Dock association is increased by Slit-Robo binding, as is the recruitment of Sos.[25] Drosophila Robo also directly interacts with the GAP Vilse or CrossGAP, which may function to down-regulate Rac activity.[26]

Netrin receptor DCC

Another way Slit-Robo signaling might mediate repulsion from the midline is by silencing the receptor of the attractive guidance cue netrin-1, Deleted in Colorectal Cancer (DCC), thereby inactivating netrin-1-mediated attraction to the midline.[8] Robo binds directly to the cytoplasmic domain of DCC and experiments with Xenopus explants have shown that this interaction silences netrin-mediated attraction; however, in vivo experiments have not yet confirmed the relevance of this mechanism for commisural axon guidance in embryos.

Interactions with commissureless

Drosophila Commissureless (Comm) is a transmembrane protein expressed in commissural neurons. Comm promotes midline crossing by down-regulating Robo. A LPSY sorting signal motif has been shown to be required for Comm to sort Robo to endosomes, preventing it from accessing the surface of the growth cone. Thus, when Comm is expressed, axons are unaffected by the presence of Slit and are able to cross the midline.[27] Comm expression is tightly regulated to ensure that axons down-regulate Robo at the correct time. In the absence of Comm, Robo is not appropriately down-regulated and all axons fail to cross the midline.

Functions

Slits mediate cell communication in many diverse systems, regulating the guidance, cell migration and polarization of many different cell types.[16]

Axon guidance

Slit-Robo interactions regulate axon guidance at the midline for commissural,[28] retinal,[29] olfactory,[30] cortical,[31] and precerebellar axons.[32] Deletions of individual robos do not phenotypically match Slit mutants, indicating that Robos1-3 play distinct, complementary but not entirely overlapping roles in axon guidance. In Drosophila, Slit interactions with Robo1 and Robo2 function together in determining whether an axon will cross the midline, and both are necessary for proper crossing.[33] Robo2 and Robo3 function together to specify the lateral position of the axon relative to the midline. The overlapping expression gradients of Robos along longitudinal tracts in the Central Nervous System (CNS) have been referred to as the "Robo-code," but it is unknown whether the formation of specific longitudinal tracts, mediated in this way by Robo, involves Slit signaling.[34] It has been speculated that homophilic and heterophilic binding among Robos may be sufficient to mediate this effect.

In vertebrates, Robo1 and Robo2 work together to mediate repulsion from Slit ligands expressed at the floor plate, while Robo3/Rig-1 has the opposite activity, and functions to promote attraction to the midline (most likely by inhibiting the other two Robo receptors, via an unknown mechanism). Mice lacking all three Robos or all three Slits exhibit a phenotype similar to the Drosophila Slit mutant.[35]

Axonal and dendritic branching

Slit2 and Slit1 have been shown to function as potential positive regulators of axon collateral formation during establishment or remodeling of neural circuits. In fact Slit2-N, an N-terminal fragment of Slit2, has been shown to induce Dorsal Root Ganglion (DRG) elongation and branching, whereas full length Slit2 antagonizes this effect.[36] In central trigeminal sensory axons, however, full length Slit2, through interactions with semaphorin receptor plexin-A4 regulates axonal branching.[37] Interactions between Slit and Robo in this process are unclear, but DRG express Robo2 and trigeminal axons express Robo1-2.[38] Slit-Robo interactions are highly implicated, however, in the dendritic development of cortical neurons in that exposure to Slit1 leads to increased dendritic outgrowth and branching while inhibition of Slit-robo interactions attenuates dendritic branching.[39]

Topographic projections

Axonal targeting by Slit-Robo appears to play an important role in the organization of topographic projections of axons which correspond to somatosensory receptive fields. In the Drosophila visual system, Slit and Robo prevent mixing of lamaina and lobula cells.[40] Variable expression of Robo receptors on Drosophila olfactory neurons controls axonal organization in the olfactory lobes.[41] In vertebrates, Slit1 plays an important role in vomeronasal organ (VNO) axonal targeting to the accessory olfactory bulb (AOB).[42] In 2009, a combination of Slit-Robo and Netrin-Frazzled signaling in Drosophila was shown to govern the establishment of myotopic maps, which describe the innervation of motorneuron dendrites in the muscle field.[43][44]

Cell migration

Slit-Robo has been shown to influence the migration of neurons and glia, leukocytes,[45] and endothelial cells.[46] Slit1 and Slit2 mediate the repulsive activity of the septum and choroid plexus which orient the migration of undifferentiated cells of the subventricular zone (SVZ) on the rostral migratory stream (RMS) to the olfactory bulb, where they differentiate into olfactory neurons.[47] The contribution of Robo signaling in this system is unclear, but it is known that migrating neuroblasts do express Robo2 and Robo3 mRNAs.[48]

During the developmental of mouse peripheral auditory system, Slit/Robo signaling imposes a restriction force on spiral ganglia neurons to ensure their precise positioning for correct spiral ganglia-cochlear hair cells innervations.[49]

Implications in disease

Cancer and vascular disease

Inhibition of Robo1, which colocalizes with von Willebrand factor in tumor endothelial cells, leads to reduced micro-vessel density and tumor mass of malignant melanoma. Slit2 is known to mediate this effect.[50] Robo4, also known as magic roundabout,[51] is an endothelial specific Robo which, upon binding Slit2, blocks Src family kinase activation, thereby inhibiting VEGF-165-induced migration and permeability in vitro and vascular leak in vivo.[52] This suggests that combined VEGF/Slit2 therapies could be useful in preventing tumor angiogenesis and vascular leak or edema after heart attack or stroke.[53]

Horizontal gaze palsy with progressive scoliosis

The homozygous Robo3 mutations have been associated with typical ophthalmologic horizontal gaze palsy with progressive scoliosis, which is characterized by oculomotor problems and general disturbances in innervation.[54]

Dyslexia

Robo1 has been implicated as one of 14 different candidate genes for dyslexia, and one of 10 that fit into a theoretical molecular network involved in neuronal migration and neurite outgrowth. Slit2 is predicted to play a role in the network.[55]

gollark: You can use the `div` function for integer division.
gollark: Ah, here's the problem! You can't use the `/` operator to divide integers.
gollark: ```haskelldata StatusOrSomething = Bought | Sold | Error```
gollark: Also, you should use an enum.
gollark: Just substitute <lang> with `haskell` or whatever language.

References
  1. Yuan W, Zhou L, Chen JH, Wu JY, Rao Y, Ornitz DM (August 1999). "The mouse SLIT family: secreted ligands for ROBO expressed in patterns that suggest a role in morphogenesis and axon guidance". Dev. Biol. 212 (2): 290–306. doi:10.1006/dbio.1999.9371. PMID 10433822.
  2. Zallen JA, Yi BA, Bargmann CI (January 1998). "The conserved immunoglobulin superfamily member SAX-3/Robo directs multiple aspects of axon guidance in C. elegans". Cell. 92 (2): 217–27. doi:10.1016/S0092-8674(00)80916-2. PMID 9458046.
  3. Howitt JA, Clout NJ, Hohenester E (November 2004). "Binding site for Robo receptors revealed by dissection of the leucine-rich repeat region of Slit". EMBO J. 23 (22): 4406–12. doi:10.1038/sj.emboj.7600446. PMC 526463. PMID 15496984.
  4. Rothberg JM, Hartley DA, Walther Z, Artavanis-Tsakonas S (December 1988). "slit: an EGF-homologous locus of D. melanogaster involved in the development of the embryonic central nervous system". Cell. 55 (6): 1047–59. doi:10.1016/0092-8674(88)90249-8. PMID 3144436.
  5. Rothberg JM, Jacobs JR, Goodman CS, Artavanis-Tsakonas S (December 1990). "slit: an extracellular protein necessary for development of midline glia and commissural axon pathways contains both EGF and LRR domains". Genes Dev. 4 (12A): 2169–87. doi:10.1101/gad.4.12a.2169. PMID 2176636.
  6. Nguyen-Ba-Charvet KT, Chédotal A (2002). "Role of Slit proteins in the vertebrate brain". J. Physiol. Paris. 96 (1–2): 91–8. doi:10.1016/S0928-4257(01)00084-5. PMID 11755787.
  7. Bashaw GJ, Kidd T, Murray D, Pawson T, Goodman CS (June 2000). "Repulsive axon guidance: Abelson and Enabled play opposing roles downstream of the roundabout receptor". Cell. 101 (7): 703–15. doi:10.1016/S0092-8674(00)80883-1. PMID 10892742.
  8. Stein E, Tessier-Lavigne M (March 2001). "Hierarchical organization of guidance receptors: silencing of netrin attraction by slit through a Robo/DCC receptor complex". Science. 291 (5510): 1928–38. Bibcode:2001Sci...291.1928S. doi:10.1126/science.1058445. PMID 11239147.
  9. Kidd T, Brose K, Mitchell KJ, Fetter RD, Tessier-Lavigne M, Goodman CS, Tear G (January 1998). "Roundabout controls axon crossing of the CNS midline and defines a novel subfamily of evolutionarily conserved guidance receptors". Cell. 92 (2): 205–15. doi:10.1016/S0092-8674(00)80915-0. PMID 9458045.
  10. Seeger M, Tear G, Ferres-Marco D, Goodman CS (March 1993). "Mutations affecting growth cone guidance in Drosophila: genes necessary for guidance toward or away from the midline". Neuron. 10 (3): 409–26. doi:10.1016/0896-6273(93)90330-T. PMID 8461134.
  11. Kidd T, Russell C, Goodman CS, Tear G (January 1998). "Dosage-sensitive and complementary functions of roundabout and commissureless control axon crossing of the CNS midline". Neuron. 20 (1): 25–33. doi:10.1016/S0896-6273(00)80431-6. PMID 9459439.
  12. Kidd T, Bland KS, Goodman CS (March 1999). "Slit is the midline repellent for the robo receptor in Drosophila". Cell. 96 (6): 785–94. doi:10.1016/S0092-8674(00)80589-9. PMID 10102267.
  13. Brose K, Bland KS, Wang KH, Arnott D, Henzel W, Goodman CS, Tessier-Lavigne M, Kidd T (March 1999). "Slit proteins bind Robo receptors and have an evolutionarily conserved role in repulsive axon guidance". Cell. 96 (6): 795–806. doi:10.1016/S0092-8674(00)80590-5. PMID 10102268.
  14. Li HS, Chen JH, Wu W, Fagaly T, Zhou L, Yuan W, Dupuis S, Jiang ZH, Nash W, Gick C, Ornitz DM, Wu JY, Rao Y (March 1999). "Vertebrate slit, a secreted ligand for the transmembrane protein roundabout, is a repellent for olfactory bulb axons". Cell. 96 (6): 807–18. doi:10.1016/S0092-8674(00)80591-7. PMID 10102269.
  15. Wu W, Wong K, Chen J, Jiang Z, Dupuis S, Wu JY, Rao Y (July 1999). "Directional guidance of neuronal migration in the olfactory system by the protein Slit". Nature. 400 (6742): 331–6. Bibcode:1999Natur.400..331W. doi:10.1038/22477. PMC 2041931. PMID 10432110.
  16. Tessier-Lavigne M, Goodman CS (November 1996). "The molecular biology of axon guidance". Science. 274 (5290): 1123–33. Bibcode:1996Sci...274.1123T. doi:10.1126/science.274.5290.1123. PMID 8895455.
  17. Hao JC, Yu TW, Fujisawa K, Culotti JG, Gengyo-Ando K, Mitani S, Moulder G, Barstead R, Tessier-Lavigne M, Bargmann CI (October 2001). "C. elegans slit acts in midline, dorsal-ventral, and anterior-posterior guidance via the SAX-3/Robo receptor". Neuron. 32 (1): 25–38. doi:10.1016/S0896-6273(01)00448-2. PMID 11604136.
  18. Battye R, Stevens A, Perry RL, Jacobs JR (June 2001). "Repellent signaling by Slit requires the leucine-rich repeats". J. Neurosci. 21 (12): 4290–8. doi:10.1523/JNEUROSCI.21-12-04290.2001. PMC 6762740. PMID 11404414.
  19. Chen JH, Wen L, Dupuis S, Wu JY, Rao Y (March 2001). "The N-terminal leucine-rich regions in Slit are sufficient to repel olfactory bulb axons and subventricular zone neurons". J. Neurosci. 21 (5): 1548–56. doi:10.1523/JNEUROSCI.21-05-01548.2001. PMC 6762944. PMID 11222645.
  20. Morlot C, Thielens NM, Ravelli RB, Hemrika W, Romijn RA, Gros P, Cusack S, McCarthy AA (September 2007). "Structural insights into the Slit-Robo complex". Proc. Natl. Acad. Sci. U.S.A. 104 (38): 14923–8. Bibcode:2007PNAS..10414923M. doi:10.1073/pnas.0705310104. PMC 1975871. PMID 17848514.
  21. Steigemann P, Molitor A, Fellert S, Jäckle H, Vorbrüggen G (February 2004). "Heparan sulfate proteoglycan syndecan promotes axonal and myotube guidance by slit/robo signaling". Curr. Biol. 14 (3): 225–30. doi:10.1016/j.cub.2004.01.006. PMID 14761655.
  22. Inatani M, Irie F, Plump AS, Tessier-Lavigne M, Yamaguchi Y (November 2003). "Mammalian brain morphogenesis and midline axon guidance require heparan sulfate". Science. 302 (5647): 1044–6. Bibcode:2003Sci...302.1044I. doi:10.1126/science.1090497. PMID 14605369.
  23. Wills Z, Emerson M, Rusch J, Bikoff J, Baum B, Perrimon N, Van Vactor D (November 2002). "A Drosophila homolog of cyclase-associated proteins collaborates with the Abl tyrosine kinase to control midline axon pathfinding". Neuron. 36 (4): 611–22. doi:10.1016/S0896-6273(02)01022-X. PMID 12441051.
  24. Wong K, Ren XR, Huang YZ, Xie Y, Liu G, Saito H, Tang H, Wen L, Brady-Kalnay SM, Mei L, Wu JY, Xiong WC, Rao Y (October 2001). "Signal transduction in neuronal migration: roles of GTPase activating proteins and the small GTPase Cdc42 in the Slit-Robo pathway". Cell. 107 (2): 209–21. doi:10.1016/S0092-8674(01)00530-X. PMID 11672528.
  25. Fan X, Labrador JP, Hing H, Bashaw GJ (September 2003). "Slit stimulation recruits Dock and Pak to the roundabout receptor and increases Rac activity to regulate axon repulsion at the CNS midline". Neuron. 40 (1): 113–27. doi:10.1016/S0896-6273(03)00591-9. PMID 14527437.
  26. Lundström A, Gallio M, Englund C, Steneberg P, Hemphälä J, Aspenström P, Keleman K, Falileeva L, Dickson BJ, Samakovlis C (September 2004). "Vilse, a conserved Rac/Cdc42 GAP mediating Robo repulsion in tracheal cells and axons". Genes Dev. 18 (17): 2161–71. doi:10.1101/gad.310204. PMC 515293. PMID 15342493.
  27. Keleman K, Rajagopalan S, Cleppien D, Teis D, Paiha K, Huber LA, Technau GM, Dickson BJ (August 2002). "Comm sorts robo to control axon guidance at the Drosophila midline". Cell. 110 (4): 415–27. doi:10.1016/S0092-8674(02)00901-7. PMID 12202032.
  28. Sabatier C; Plump AS; Le Ma; Brose K; Tamada A; Murakami F; Lee EY; Tessier-Lavigne M (April 2004). "The divergent Robo family protein rig-1/Robo3 is a negative regulator of slit responsiveness required for midline crossing by commissural axons". Cell. 117 (2): 157–69. doi:10.1016/S0092-8674(04)00303-4. PMID 15084255.
  29. Hussain SA, Piper M, Fukuhara N, Strochlic L, Cho G, Howitt JA, Ahmed Y, Powell AK, Turnbull JE, Holt CE, Hohenester E (December 2006). "A molecular mechanism for the heparan sulfate dependence of slit-robo signaling". J. Biol. Chem. 281 (51): 39693–8. doi:10.1074/jbc.M609384200. PMC 3680705. PMID 17062560.
  30. Nguyen-Ba-Charvet KT, Plump AS, Tessier-Lavigne M, Chedotal A (July 2002). "Slit1 and slit2 proteins control the development of the lateral olfactory tract". J. Neurosci. 22 (13): 5473–80. doi:10.1523/JNEUROSCI.22-13-05473.2002. PMC 6758232. PMID 12097499.
  31. Shu T, Sundaresan V, McCarthy MM, Richards LJ (September 2003). "Slit2 guides both precrossing and postcrossing callosal axons at the midline in vivo". J. Neurosci. 23 (22): 8176–84. doi:10.1523/JNEUROSCI.23-22-08176.2003. PMC 6740498. PMID 12954881.
  32. Marillat V, Sabatier C, Failli V, Matsunaga E, Sotelo C, Tessier-Lavigne M, Chédotal A (July 2004). "The slit receptor Rig-1/Robo3 controls midline crossing by hindbrain precerebellar neurons and axons". Neuron. 43 (1): 69–79. doi:10.1016/j.neuron.2004.06.018. PMID 15233918.
  33. Simpson JH, Kidd T, Bland KS, Goodman CS (December 2000). "Short-range and long-range guidance by slit and its Robo receptors. Robo and Robo2 play distinct roles in midline guidance". Neuron. 28 (3): 753–66. doi:10.1016/S0896-6273(00)00151-3. PMID 11163264.
  34. Simpson JH, Bland KS, Fetter RD, Goodman CS (December 2000). "Short-range and long-range guidance by Slit and its Robo receptors: a combinatorial code of Robo receptors controls lateral position". Cell. 103 (7): 1019–32. doi:10.1016/S0092-8674(00)00206-3. PMID 11163179.
  35. Long H, Sabatier C, Ma L, Plump A, Yuan W, Ornitz DM, Tamada A, Murakami F, Goodman CS, Tessier-Lavigne M (April 2004). "Conserved roles for Slit and Robo proteins in midline commissural axon guidance". Neuron. 42 (2): 213–23. doi:10.1016/S0896-6273(04)00179-5. PMID 15091338.
  36. Nguyen Ba-Charvet KT, Brose K, Ma L, Wang KH, Marillat V, Sotelo C, Tessier-Lavigne M, Chédotal A (June 2001). "Diversity and specificity of actions of Slit2 proteolytic fragments in axon guidance". J. Neurosci. 21 (12): 4281–9. doi:10.1523/JNEUROSCI.21-12-04281.2001. PMC 6762758. PMID 11404413.
  37. Miyashita T, Yeo SY, Hirate Y, Segawa H, Wada H, Little MH, Yamada T, Takahashi N, Okamoto H (August 2004). "PlexinA4 is necessary as a downstream target of Islet2 to mediate Slit signaling for promotion of sensory axon branching". Development. 131 (15): 3705–15. doi:10.1242/dev.01228. PMID 15229183.
  38. Wang KH, Brose K, Arnott D, Kidd T, Goodman CS, Henzel W, Tessier-Lavigne M (March 1999). "Biochemical purification of a mammalian slit protein as a positive regulator of sensory axon elongation and branching". Cell. 96 (6): 771–84. doi:10.1016/S0092-8674(00)80588-7. PMID 10102266.
  39. Whitford KL, Marillat V, Stein E, Goodman CS, Tessier-Lavigne M, Chédotal A, Ghosh A (January 2002). "Regulation of cortical dendrite development by Slit-Robo interactions". Neuron. 33 (1): 47–61. doi:10.1016/S0896-6273(01)00566-9. PMID 11779479.
  40. Tayler TD, Robichaux MB, Garrity PA (December 2004). "Compartmentalization of visual centers in the Drosophila brain requires Slit and Robo proteins". Development. 131 (23): 5935–45. doi:10.1242/dev.01465. PMC 1201521. PMID 15525663.
  41. Jhaveri D, Saharan S, Sen A, Rodrigues V (May 2004). "Positioning sensory terminals in the olfactory lobe of Drosophila by Robo signaling". Development. 131 (9): 1903–12. doi:10.1242/dev.01083. PMID 15056612.
  42. Cloutier JF, Sahay A, Chang EC, Tessier-Lavigne M, Dulac C, Kolodkin AL, Ginty DD (October 2004). "Differential requirements for semaphorin 3F and Slit-1 in axonal targeting, fasciculation, and segregation of olfactory sensory neuron projections". J. Neurosci. 24 (41): 9087–96. doi:10.1523/JNEUROSCI.2786-04.2004. PMC 6730055. PMID 15483127.
  43. Brierley DJ, Blanc E, Reddy OV, Vijayraghavan K, Williams DW (September 2009). "Dendritic targeting in the leg neuropil of Drosophila: the role of midline signalling molecules in generating a myotopic map". PLOS Biol. 7 (9): e1000199. doi:10.1371/journal.pbio.1000199. PMC 2737123. PMID 19771147.
  44. Mauss A, Tripodi M, Evers JF, Landgraf M (September 2009). "Midline signalling systems direct the formation of a neural map by dendritic targeting in the Drosophila motor system". PLOS Biol. 7 (9): e1000200. doi:10.1371/journal.pbio.1000200. PMC 2736389. PMID 19771146.
  45. Wong K, Park HT, Wu JY, Rao Y (October 2002). "Slit proteins: molecular guidance cues for cells ranging from neurons to leukocytes". Curr. Opin. Genet. Dev. 12 (5): 583–91. doi:10.1016/S0959-437X(02)00343-X. PMID 12200164.
  46. Carmeliet P, Tessier-Lavigne M (July 2005). "Common mechanisms of nerve and blood vessel wiring". Nature. 436 (7048): 193–200. Bibcode:2005Natur.436..193C. doi:10.1038/nature03875. PMID 16015319.
  47. Nguyen-Ba-Charvet KT, Picard-Riera N, Tessier-Lavigne M, Baron-Van Evercooren A, Sotelo C, Chédotal A (February 2004). "Multiple roles for slits in the control of cell migration in the rostral migratory stream". J. Neurosci. 24 (6): 1497–506. doi:10.1523/JNEUROSCI.4729-03.2004. PMC 6730320. PMID 14960623.
  48. Marillat V, Cases O, Nguyen-Ba-Charvet KT, Tessier-Lavigne M, Sotelo C, Chédotal A (January 2002). "Spatiotemporal expression patterns of slit and robo genes in the rat brain". J. Comp. Neurol. 442 (2): 130–55. doi:10.1002/cne.10068. PMID 11754167.
  49. S.Z. Wang, L.A. Ibrahim, Y.J. Kim, D.A. Gibson, H.C. Leung, W. Yuan, K.K. Zhang, H.W. Tao, L. Ma, L.I. Zhang Slit/Robo signaling mediates spatial positioning of spiral ganglion neurons during development of cochlear innervation J. Neurosci., 30 (2013), pp. 12242–12254
  50. Wang B, Xiao Y, Ding BB, Zhang N, Yuan X, Gui L, Qian KX, Duan S, Chen Z, Rao Y, Geng JG (July 2003). "Induction of tumor angiogenesis by Slit-Robo signaling and inhibition of cancer growth by blocking Robo activity". Cancer Cell. 4 (1): 19–29. doi:10.1016/S1535-6108(03)00164-8. PMID 12892710.
  51. Jones CA, Nishiya N, London NR, Zhu W, Sorensen LK, Chan AC, Lim CJ, Chen H, Zhang Q, Schultz PG, Hayallah AM, Thomas KR, Famulok M, Zhang K, Ginsberg MH, Li DY (November 2009). "Slit2-Robo4 signalling promotes vascular stability by blocking Arf6 activity". Nat. Cell Biol. 11 (11): 1325–31. doi:10.1038/ncb1976. PMC 2854659. PMID 19855388.
  52. Jones CA, London NR, Chen H, Park KW, Sauvaget D, Stockton RA, Wythe JD, Suh W, Larrieu-Lahargue F, Mukouyama YS, Lindblom P, Seth P, Frias A, Nishiya N, Ginsberg MH, Gerhardt H, Zhang K, Li DY (April 2008). "Robo4 stabilizes the vascular network by inhibiting pathologic angiogenesis and endothelial hyperpermeability". Nat. Med. 14 (4): 448–53. doi:10.1038/nm1742. PMC 2875252. PMID 18345009.
  53. Acevedo LM, Weis SM, Cheresh DA (April 2008). "Robo4 counteracts VEGF signaling". Nat. Med. 14 (4): 372–3. doi:10.1038/nm0408-372. PMID 18391935.
  54. Volk AE, Carter O, Fricke J, Herkenrath P, Poggenborg J, Borck G, Demant AW, Ivo R, Eysel P, Kubisch C, Neugebauer A (2011). "Horizontal gaze palsy with progressive scoliosis: three novel ROBO3 mutations and descriptions of the phenotypes of four patients". Mol. Vis. 17: 1978–86. PMC 3154129. PMID 21850172.
  55. Poelmans G, Buitelaar JK, Pauls DL, Franke B (April 2011). "A theoretical molecular network for dyslexia: integrating available genetic findings". Mol. Psychiatry. 16 (4): 365–82. doi:10.1038/mp.2010.105. PMID 20956978.

Further reading
This article is issued from Wikipedia. The text is licensed under Creative Commons - Attribution - Sharealike. Additional terms may apply for the media files.