Pyrimidine oxygenase

Pyrimidine oxygenase (EC 1.14.99.46, RutA) is an enzyme with systematic name uracil,FMNH2:oxygen oxidoreductase (uracil hydroxylating, ring-opening) .[1][2] This enzyme catalyses the following chemical reaction

(1) uracil + FMNH2 + O2 (Z)-3-ureidoacrylate peracid + FMN
(2) thymine + FMNH2 + O2 (Z)-2-methylureidoacrylate peracid + FMN
Pyrimidine oxygenase
Identifiers
EC number1.14.99.46
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum

In vitro the product (Z)-3-ureidoacrylate peracid is spontaneously reduced to ureidoacrylate.

References

  1. Mukherjee T, Zhang Y, Abdelwahed S, Ealick SE, Begley TP (April 2010). "Catalysis of a flavoenzyme-mediated amide hydrolysis". Journal of the American Chemical Society. 132 (16): 5550–1. doi:10.1021/ja9107676. PMC 2873085. PMID 20369853.
  2. Kim KS, Pelton JG, Inwood WB, Andersen U, Kustu S, Wemmer DE (August 2010). "The Rut pathway for pyrimidine degradation: novel chemistry and toxicity problems". Journal of Bacteriology. 192 (16): 4089–102. doi:10.1128/JB.00201-10. PMC 2916427. PMID 20400551.
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