Jaswant Singh–Bhattacharji stain

Jaswant Singh–Bhattacharji stain, commonly referred to as JSB stain, is a rapid staining method for detection of malaria.[1][2] It is useful for the diagnosis of malaria in thick smear samples of blood.[3] The JSB stain is commonly used throughout India, but rarely used in other countries.[4]

Composition

The JSB stain consists of two solutions which are used in sequence to stain various parts of the sample. The first solution consists of methylene blue, potassium dichromate, and sulfuric acid diluted in water. This solution is heated for several hours to oxidize the methylene blue. The second solution is eosin dissolved in water.[5]

gollark: Apparently my `.config` folder is 1GB.
gollark: ```Filesystem 1K-blocks Used Available Use% Mounted ondev 4049896 0 4049896 0% /devrun 4093740 1156 4092584 1% /run/dev/mapper/arch-root 62914560 36713744 25937136 59% /tmpfs 4093740 32672 4061068 1% /dev/shmtmpfs 4093740 0 4093740 0% /sys/fs/cgrouptmpfs 4093740 8236 4085504 1% /tmp/dev/sda1 1046512 72504 974008 7% /boot/dev/mapper/arch-home 163371072 142836388 20534684 88% /hometmpfs 818748 32 818716 1% /run/user/1000```
gollark: Well, my server holds other stuff, and it has 1TB.
gollark: I had to clean out my home folder a while ago.
gollark: Yep!

See also

References
  1. Singh, Jaswant; Bhattacharji, S; WHO Expert Committee on Malaria; WHO Malaria Conference in Equatorial Africa (1950 : Kampala, Uganda) (1950). "Rapid staining method of blood smears / Jaswant Singh, Bhattacharji". Geneva : World Health Organization. Cite journal requires |journal= (help)
  2. Manwell, RD; Feigelson, P (June 1948). "A modified method of preparing the J. S. B. stain". The Journal of Laboratory and Clinical Medicine. 33 (6): 777–82. PMID 18865757.
  3. Annam, Vamseedhar; Mohan, ChakkiralaNalini; Mrinalini, VazhayilRamunny (2013). "Rapid detection of malaria parasite by toluidine blue method: A new staining method". Journal of Global Infectious Diseases. 5 (4): 153. doi:10.4103/0974-777X.122008. PMC 3958985. PMID 24672177.
  4. Malaria Microscopy Quality Assurance Manual. World Health Organization. 2009. p. 29. ISBN 9789290614227.
  5. US Dept. of the Army (1951). Methods for Medical Laboratory Technicians. US Government Printing Office. pp. 610–611.
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