Field stain

Field stain is a histological method for staining of blood smears. It is used for staining thick blood films in order to discover malarial parasites. Field's stain is a version of a Romanowsky stain, used for rapid processing of the specimens.[1]

Micrograph of a Field stain showing malignant melanoma.

Field's stain consists of two parts - Field's stain A is methylene blue and Azure 1 dissolved in phosphate buffer solution; Field's stain B is Eosin Y in buffer solution. Field stain is named after physician John William Field, who developed it in 1941.[2]

Additional images
  • Colorectal adenocarcinoma. Field stain.
  • Granuloma. Field stain.
gollark: You can't overthrow God, they've been dead since 1996.
gollark: Of course. You are unlikely to implode.
gollark: https://media.discordapp.net/attachments/426116061415342080/925432567417696396/Screenshot_20211224-143635_YouTube.jpg
gollark: Optimal bees, even.
gollark: But some of the bees are superior bees.

References
  1. Chunge, CN.; Ngige, S.; Bwibo, CR.; Mulega, PC.; Kilonzo, JF.; Kibati, F.; Owate, J. (Aug 1989). "A rapid staining technique for Leishmania parasites in splenic aspirate smears". Ann Trop Med Parasitol. 83 (4): 361–4. PMID 2481429.
  2. "Obituaries" (PDF). Medical Journal of Malaysia. June 1981. Retrieved 4 May 2015.


This article is issued from Wikipedia. The text is licensed under Creative Commons - Attribution - Sharealike. Additional terms may apply for the media files.