tRNA (guanine-N7-)-methyltransferase

In enzymology, a tRNA (guanine-N7-)-methyltransferase (EC 2.1.1.33) is an enzyme that catalyzes the chemical reaction

S-adenosyl-L-methionine + tRNA S-adenosyl-L-homocysteine + tRNA containing N7-methylguanine
tRNA (guanine-N7-)-methyltransferase
Identifiers
EC number2.1.1.33
CAS number37257-00-4
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO

Thus, the two substrates of this enzyme are S-adenosyl methionine and tRNA, whereas its two products are S-adenosylhomocysteine and tRNA containing N7-methylguanine.

This enzyme belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. The systematic name of this enzyme class is S-adenosyl-L-methionine:tRNA (guanine-N7-)-methyltransferase. Other names in common use include transfer ribonucleate guanine 7-methyltransferase, 7-methylguanine transfer ribonucleate methylase, tRNA guanine 7-methyltransferase, N7-methylguanine methylase, and S-adenosyl-L-methionine:tRNA (guanine-7-N-)-methyltransferase.

Structural studies

As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 1YZH and 2FCA.

gollark: Which is... no longer accessible, I should really bring my website back up.
gollark: You're right, I should add tau support to my RPN calculator.
gollark: Yes, which is mildly more annoying than saying τ radians.
gollark: τ radians is a full turn of a circle. 1/4τ is a quarter circle. This is somewhat easier than with pi.
gollark: Radians!

References
    • Hurwitz J, Gold M, Anders M (1964). "The Enzymatic Methylation of Ribonucleic Acid and Deoxyribonucleic Acid. 3. Purification of Soluble Ribonucleic Acid-Methylating Enzymes". J. Biol. Chem. 239: 3462–3473. PMID 14245404.
    • Bjork GR, Svensson I (1969). "Studies on microbial RNA. Fractionation of tRNA methylases from Saccharomyces cerevisiae". Eur. J. Biochem. 9 (2): 207–215. doi:10.1111/j.1432-1033.1969.tb00596.x. PMID 4896260.
    • Aschhoff HJ, Elten H, Arnold HH, Mahal G, Kersten W, Kersten H (1976). "7-Methylguanine specific tRNA-methyltransferase from Escherichia coli". Nucleic Acids Res. 3 (11): 3109–22. doi:10.1093/nar/3.11.3109. PMC 343155. PMID 794833.
    • Salvatore F, Traboni C, Colonna A, Ciliberto G, Paoletta C, Cimino F (1980). Cavallini D, Gaul GE, Zappia V (eds.). Natural Sulfur Compounds. New York: Plenum Press. p. 25.


    This article is issued from Wikipedia. The text is licensed under Creative Commons - Attribution - Sharealike. Additional terms may apply for the media files.