Phthalyl amidase

In enzymology, a phthalyl amidase (EC 3.5.1.79) is an enzyme that catalyzes the chemical reaction

a phthalylamide + H2O phthalic acid + a substituted amine
phthalyl amidase
Identifiers
EC number3.5.1.79
CAS number169150-79-2
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO

Thus, the two substrates of this enzyme are phthalylamide and H2O, whereas its two products are phthalic acid and substituted amine.

This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is phthalyl-amide amidohydrolase.

References

    • M (1996). "Discovery, purification, and properties of o-phthalyl amidase from Xanthobacter agilis". J. Mol. Catal., B Enzym. 2: 53–69. doi:10.1016/1381-1177(96)00011-2.
    • Zmijewski MJ; Briggs, Barbara S.; Evans, Robert; Muth, William L.; Vangala, Surya; Zmijewski, Milton J. (1996). "o-Phthalyl amidase in the synthesis of Loracarbef: process development using this novel biocatalyst". Biotechnol. Lett. 18: 875–880. doi:10.1007/BF00154613.
    • Costello C, Kreuzman A, Zmijewski M (1996). "Selective deprotection of phthalyl protected proteins". Tetrahedron Lett. 37: 7469–7472. doi:10.1016/0040-4039(96)01752-2.
    • Briggs BS, Zmijewski MJ (1995). "Enzyme from microbial source: phthalyl amidase". Chem. Abstr. 123: 25010.


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