Hydroxyisourate hydrolase
In enzymology, a hydroxyisourate hydrolase (EC 3.5.2.17) is an enzyme that catalyzes the chemical reaction
- 5-hydroxyisourate + H2O 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate
| Hydroxyisourate hydrolase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 3.5.2.17 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
Thus, the two substrates of this enzyme are 5-hydroxyisourate and H2O, whereas its product is 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate.
This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in cyclic amides. The systematic name of this enzyme class is 5-hydroxyisourate amidohydrolase. Other names in common use include HIUHase, and 5-hydroxyisourate hydrolase. This enzyme participates in purine metabolism.
Structural studies
As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 2H1X and 2H6U.
gollark: Or longer.
gollark: The timeline is probably a few hundred years to run out of uranium.
gollark: *Technically* with a finite amount you'll eventually run out, but advancing technology should mean it would be easy to replace it anyway.
gollark: You don't need to. There's enough uranium.
gollark: We have enough for 70 years of current production available, and the many, many ways to get more or use existing stuff more efficiently have just been ignored because they aren't needed now.
References
- Raychaudhuri A, Tipton PA (2003). "A familiar motif in a new context: the catalytic mechanism of hydroxyisourate hydrolase". Biochemistry. 42 (22): 6848–52. doi:10.1021/bi034137o. PMID 12779339.
- Raychaudhuri A, Tipton PA (2002). "Cloning and expression of the gene for soybean hydroxyisourate hydrolase. Localization and implications for function and mechanism". Plant Physiol. 130 (4): 2061–8. doi:10.1104/pp.011049. PMC 166717. PMID 12481089.
- Sarma AD, Serfozo P, Kahn K, Tipton PA (1999). "Identification and purification of hydroxyisourate hydrolase, a novel ureide-metabolizing enzyme". J. Biol. Chem. 274 (48): 33863–5. doi:10.1074/jbc.274.48.33863. PMID 10567345.
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