Mycothiol-dependent formaldehyde dehydrogenase
In enzymology, a mycothiol-dependent formaldehyde dehydrogenase (EC 1.1.1.306) is an enzyme that catalyzes the chemical reaction
- formaldehyde + mycothiol + NAD+ S-formylmycothiol + NADH + 2 H+
| mycothiol-dependent formaldehyde dehydrogenase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 1.1.1.306 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
The 3 substrates of this enzyme are formaldehyde, mycothiol, and NAD+, whereas its 3 products are S-formylmycothiol, NADH, and H+.[1][2][3][4] This enzyme catalyses the following chemical reaction
This enzyme belongs to the family of oxidoreductases, specifically those acting on the aldehyde or oxo group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is formaldehyde:NAD+ oxidoreductase (mycothiol-formylating). This enzyme is also called NAD/factor-dependent formaldehyde dehydrogenase or S-(hydroxymethyl)mycothiol dehydrogenase.
References
- Misset-Smits M, van Ophem PW, Sakuda S, Duine JA (June 1997). "Mycothiol, 1-O-(2'-[N-acetyl-L-cysteinyl]amido-2'-deoxy-alpha-D-glucopyranosyl)-D- myo-inositol, is the factor of NAD/factor-dependent formaldehyde dehydrogenase". FEBS Letters. 409 (2): 221–2. doi:10.1016/S0014-5793(97)00510-3. PMID 9202149.
- Norin A, Van Ophem PW, Piersma SR, Persson B, Duine JA, Jörnvall H (September 1997). "Mycothiol-dependent formaldehyde dehydrogenase, a prokaryotic medium-chain dehydrogenase/reductase, phylogenetically links different eukaroytic alcohol dehydrogenases--primary structure, conformational modelling and functional correlations". European Journal of Biochemistry. 248 (2): 282–9. doi:10.1111/j.1432-1033.1997.00282.x. PMID 9346279.
- Vogt RN, Steenkamp DJ, Zheng R, Blanchard JS (September 2003). "The metabolism of nitrosothiols in the Mycobacteria: identification and characterization of S-nitrosomycothiol reductase". The Biochemical Journal. 374 (Pt 3): 657–66. doi:10.1042/BJ20030642. PMC 1223637. PMID 12809551.
- Rawat M, Av-Gay Y (April 2007). "Mycothiol-dependent proteins in actinomycetes". FEMS Microbiology Reviews. 31 (3): 278–92. doi:10.1111/j.1574-6976.2006.00062.x. PMID 17286835.
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