Iodotyrosine deiodinase

Iodotyrosine deiodinase, also known as iodotyrosine dehalogenase 1, is a type of deiodinase enzyme that scavenges iodide by removing it from iodinated tyrosine residues in the thyroid gland.[4] These iodinated tyrosines are produced during thyroid hormone biosynthesis.[5] The iodide that is scavenged by iodotyrosine deiodinase is necessary to again synthesize the thyroid hormones.[6] After synthesis, the thyroid hormones circulate through the body to regulate metabolic rate, protein expression, and body temperature.[7] Iodotyrosine deiodinase is thus necessary to keep levels of both iodide and thyroid hormones in balance.

Iodotyrosine deiodinase
Identifiers
EC number1.21.1.1
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
IYD
Available structures
PDBOrtholog search: PDBe RCSB
Identifiers
AliasesIYD, iodotyrosine deiodinase, C6orf71, DEHAL1, TDH4, dJ422F24.1, IYD-1
External IDsOMIM: 612025 MGI: 1917587 HomoloGene: 12352 GeneCards: IYD
Gene location (Human)
Chr.Chromosome 6 (human)[1]
Band6q25.1Start150,368,892 bp[1]
End150,405,969 bp[1]
Orthologs
SpeciesHumanMouse
Entrez

389434

70337

Ensembl

ENSG00000009765

n/a

UniProt

Q6PHW0

Q9DCX8

RefSeq (mRNA)

NM_001164694
NM_001164695
NM_203395
NM_001318495

NM_027391

RefSeq (protein)

NP_001158166
NP_001158167
NP_001305424
NP_981932

NP_081667

Location (UCSC)Chr 6: 150.37 – 150.41 Mbn/a
PubMed search[2][3]
Wikidata
View/Edit HumanView/Edit Mouse

Dehalogenation in aerobic organisms is usually done through oxidation and hydrolysis;[8] however, iodotyrosine deiodinase uses reductive dehalogenation. Iodotyrosine deiodinase and iodothyronine deiodinase have been determined as the only two known enzymes to catalyze reductive dehalogenation in mammals.[7] Although these two enzymes perform similar functions, they are structurally and mechanistically different. Iodothyronine deiodinase (not the enzyme that is the topic of this article) uses a selenocysteine active site for catalysis, is a member of the thioredoxin superfamily, and removes iodide only when the substrate is in a double-tyrosine form.[9] By contrast, iodotyrosine deiodinase (the topic enzyme) does not require selenocysteine or cysteine for catalysis,[10] is part of the NADH oxidase/flavin reductase superfamily,[11][12] and removes iodide when the substrate is a single amino acid.[13] Research on iodotyrosine deiodinase has historically been variable and slow due to its lack of stability and arduous purification.[14] Only recently has this enzyme been studied more deeply.[7]

Structure
Structure of human iodotyrosine deiodinase generated from PDB entry 4TTB.[15]

The gene encoding this enzyme has been recently identified.[11][12] The sequence of amino acids of iodotyrosine deiodinase is highly conserved among mammals and contains three domains.[7] Iodotyrosine deiodinase is a membrane protein, with the N-terminus functioning as a membrane anchor.[10][16] It forms a dimer that is domain-swapped.[13] Initially, iodotyrosine deiodinase was thought to contain only one flavin mononucleotide (FMN) in each dimer,[17] but now iodotyrosine deiodinase is believed to have two FMN molecules for each homodimer.[13] The enzyme has a characteristic α-β fold that all proteins from the NADH oxidase/flavin reductase superfamily have as well. Within the dimer interface, there are two equivalent active sites, each made from residues from both subunits. Thus, subunit association must be required for FMN binding and catalysis. Substrate binding causes a conformational change in the enzyme in order to close the active site, protecting the substrate and flavin from the solvent.[13]

Function

Iodotyrosine deiodinase facilitates iodide salvage in the thyroid by catalyzing deiodination of mono- and diiodotyrosine, the halogenated byproducts of thyroid hormone production.[12] Iodide is also an important micronutrient in the biosynthesis of thyroid hormone, creating a cycle of iodide use in the thyroid.[13] Iodide homeostasis within the thyroid gland is essential for producing thyroid hormone at appropriate rates. Thus, iodide levels must be regulated in order to keep thyroid hormones, and ultimately the organism's metabolic rate and overall health, in good status.[18]

Within the thyroid follicular cell, thyroglobulin is hydrolyzed to form thyroid hormones and mono- and diiodotyrosine. The thyroid hormones are released into the bloodstream and the iodinated tyrosines are recycled. However, the breakdown of thyroglobulin produces 6-7 fold more iodinated tyrosines than thyroid hormone.[7] Iodotyrosine deiodinase salvages the iodide from the deiodination of the iodinated tyrosines.[19] Iodotyrosine deiodinase is located on the apical plasma membrane of the thyroid colloid, where mono- and diiodotyrosine are produced from this breakdown of thyroglobulin. Without iodotyrosine deiodinase activity, the iodide would be excreted with the amino acid tyrosine and thyroid hormone biosynthesis would be reduced.[13]

The enzymatic activity of iodotyrosine deiodinase has also been known to exist in the tissues of the liver and kidneys as well;[20] however, the physiological significance of these findings is not yet clear.[7]

Mechanism

Iodotyrosine deiodinase catalyzes mono- and diiodotyrosine deiodination. The reaction is NADPH-dependent.[16] Flavin mononucleotide (FMN) is a cofactor.[21] Although flavin is commonly utilized in various catalytic reactions,[22] its use in this reductive dehalogenation is unique and not yet fully understood.[13] It is also still unclear if the enzyme mechanism utilizes a two electron transfer reaction or a series of one electron transfers. Although further research must be done to determine details of this mechanism, recent evidence seems to suggest that iodotyrosine deiodinase acts through one electron transfer reactions.[7]

Iodotyrosine deiodinase reaction scheme.[7]

Clinical significance

Mutations in the gene encoding iodotyrosine deiodinase can affect enzyme function and be detrimental to human health. Iodide is an essential micronutrient for health in mammals.[23] Low levels of iodide either through the diet or through iodide metabolism are associated with hypothyroidism, mental retardation, goiter, and developmental defects.[4][7][18] Because iodotyrosine deiodinase is responsible for scavenging iodide, mutations in this enzyme result in iodide deficiency.[24]

The resulting high blood and urine concentrations of iodotyrosine can be used as a measure for diagnosis, as the iodide is not removed from the tyrosine residues effectively.[25] In some countries, newborn babies are tested for congenital hypothyroidism and treated immediately if the disease is detected, safely preventing the development of mental retardation.[26] However, mutations of iodotyrosine deiodinase are often not detected until after developmental damage has already occurred.[18] Furthermore, these mutations may not be specifically detected using standard thyroid function tests.[18] To combat this issue, a sensitive assay has recently been created that measures the amounts of mono- and diiodotyrosine in the urine.[25]

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See also

References
  1. GRCh38: Ensembl release 89: ENSG00000009765 - Ensembl, May 2017
  2. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  3. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. Moreno JC, Visser TJ (June 2010). "Genetics and phenomics of hypothyroidism and goiter due to iodotyrosine deiodinase (DEHAL1) gene mutations". Molecular and Cellular Endocrinology. 322 (1–2): 91–8. doi:10.1016/j.mce.2010.03.010. PMID 20298747.
  5. Green WL (August 1968). "Inhibition of thyroidal iodotyrosine deiodination by tyrosine analogues". Endocrinology. 83 (2): 336–47. doi:10.1210/endo-83-2-336. PMID 5668272.
  6. Querido A, Stanbury JB, Kassenaar AA, Meijer JW (August 1956). "The metabolism of iodotyrosines. III. Di-iodotyrosine deshalogenating activity of human thyroid tissue". The Journal of Clinical Endocrinology and Metabolism. 16 (8): 1096–101. doi:10.1210/jcem-16-8-1096. PMID 13345866.
  7. Rokita SE, Adler JM, McTamney PM, Watson JA (September 2010). "Efficient use and recycling of the micronutrient iodide in mammals". Biochimie. 92 (9): 1227–35. doi:10.1016/j.biochi.2010.02.013. PMC 2888766. PMID 20167242.
  8. Häggblom M, Bossert I (2003). Dehalogenation: Microbial Processes and Environmental Applications. Boston: Kluwer Academic Publishers. p. 520. ISBN 978-1-4757-7807-6.
  9. Callebaut I, Curcio-Morelli C, Mornon JP, Gereben B, Buettner C, Huang S, Castro B, Fonseca TL, Harney JW, Larsen PR, Bianco AC (September 2003). "The iodothyronine selenodeiodinases are thioredoxin-fold family proteins containing a glycoside hydrolase clan GH-A-like structure". The Journal of Biological Chemistry. 278 (38): 36887–96. doi:10.1074/jbc.M305725200. PMID 12847093.
  10. Watson JA, McTamney PM, Adler JM, Rokita SE (March 2008). "Flavoprotein iodotyrosine deiodinase functions without cysteine residues". ChemBioChem. 9 (4): 504–6. doi:10.1002/cbic.200700562. PMID 18228228.
  11. Gnidehou S, Caillou B, Talbot M, Ohayon R, Kaniewski J, Noël-Hudson MS, Morand S, Agnangji D, Sezan A, Courtin F, Virion A, Dupuy C (October 2004). "Iodotyrosine dehalogenase 1 (DEHAL1) is a transmembrane protein involved in the recycling of iodide close to the thyroglobulin iodination site". FASEB Journal. 18 (13): 1574–6. doi:10.1096/fj.04-2023fje. PMID 15289438.
  12. Friedman JE, Watson JA, Lam DW, Rokita SE (February 2006). "Iodotyrosine deiodinase is the first mammalian member of the NADH oxidase/flavin reductase superfamily". The Journal of Biological Chemistry. 281 (5): 2812–9. doi:10.1074/jbc.M510365200. PMID 16316988.
  13. Thomas SR, McTamney PM, Adler JM, Laronde-Leblanc N, Rokita SE (July 2009). "Crystal structure of iodotyrosine deiodinase, a novel flavoprotein responsible for iodide salvage in thyroid glands". The Journal of Biological Chemistry. 284 (29): 19659–67. doi:10.1074/jbc.M109.013458. PMC 2740591. PMID 19436071.
  14. Rosenberg IN, Goswami A (1 January 1984). "Iodotyrosine deiodinase from bovine thyroid". Methods in Enzymology. 107: 488–500. doi:10.1016/0076-6879(84)07033-6. ISBN 9780121820077. PMID 6503724.
  15. "RCSB PDB - Search Results". www.rcsb.org. RCSB Protein Data Banck. Retrieved 1 March 2016.
  16. "Entrez Gene: IYD Iodotyrosine deiodinase". Entrez Gene. United States National Library of Medicine.
  17. Rosenberg IN, Goswami A (December 1979). "Purification and characterization of a flavoprotein from bovine thyroid with iodotyrosine deiodinase activity". The Journal of Biological Chemistry. 254 (24): 12318–25. PMID 500717.
  18. Moreno JC, Klootwijk W, van Toor H, Pinto G, D'Alessandro M, Lèger A, Goudie D, Polak M, Grüters A, Visser TJ (April 2008). "Mutations in the iodotyrosine deiodinase gene and hypothyroidism". The New England Journal of Medicine. 358 (17): 1811–8. doi:10.1056/NEJMoa0706819. PMID 18434651.
  19. Knobel M, Medeiros-Neto G (August 2003). "An outline of inherited disorders of the thyroid hormone generating system". Thyroid. 13 (8): 771–801. doi:10.1089/105072503768499671. PMID 14558921.
  20. Choufoer JC, Kassenaar AA, Querido A (July 1960). "The syndrome of congenital hypothyroidism with defective dehalogenation of iodotyrosines. Further observations and a discussion of the pathophysiology". The Journal of Clinical Endocrinology and Metabolism. 20 (7): 983–1003. doi:10.1210/jcem-20-7-983. PMID 13810029.
  21. Goswami A, Rosenberg IN (December 1979). "Characterization of a flavoprotein iodotyrosine deiodinase from bovine thyroid. Flavin nucleotide binding and oxidation-reduction properties". The Journal of Biological Chemistry. 254 (24): 12326–30. PMID 500718.
  22. Mansoorabadi SO, Thibodeaux CJ, Liu HW (August 2007). "The diverse roles of flavin coenzymes--nature's most versatile thespians". The Journal of Organic Chemistry. 72 (17): 6329–42. doi:10.1021/jo0703092. PMC 2519020. PMID 17580897.
  23. Eastman CJ, Zimmermann M (1 January 2000). De Groot LJ, Beck-Peccoz P, Chrousos G, Dungan K, Grossman A, Hershman JM, Koch C, McLachlan R, New M (eds.). The Iodine Deficiency Disorders. South Dartmouth (MA): MDText.com, Inc. PMID 25905411.
  24. Lever EG, Medeiros-Neto GA, DeGroot LJ (1983). "Inherited disorders of thyroid metabolism". Endocrine Reviews. 4 (3): 213–39. doi:10.1210/edrv-4-3-213. PMID 6354701.
  25. Afink G, Kulik W, Overmars H, de Randamie J, Veenboer T, van Cruchten A, Craen M, Ris-Stalpers C (December 2008). "Molecular characterization of iodotyrosine dehalogenase deficiency in patients with hypothyroidism" (PDF). The Journal of Clinical Endocrinology and Metabolism. 93 (12): 4894–901. doi:10.1210/jc.2008-0865. PMID 18765512.
  26. New England congenital hypothyroidism collaborative (November 1981). "Effects of neonatal screening for hypothyroidism: prevention of mental retardation by treatment before clinical manifestations". Lancet. 2 (8255): 1095–8. doi:10.1016/s0140-6736(81)91287-3. PMID 6118534.

Further reading

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