Florence Bell (scientist)

Florence Ogilvy Bell (1 May 1913 – 23 November 2000), later Florence Sawyer, was a British scientist who contributed to the discovery of the structure of DNA. She was an X-ray crystallographer in the lab of William Astbury. In 1938 they published a paper in Nature that described the structure of DNA as a "Pile of Pennies".[3]

Florence Ogilvy Bell
c. 1938
Born1 May 1913[1]
London, England[2]
Died23 November 2000
Alma materUniversity of Leeds
University of Cambridge
Scientific career
InstitutionsUniversity of Leeds
University of Manchester
ThesisX-ray and related studies of the structure of the proteins and nucleic acids
Doctoral advisorWilliam Astbury

Early life

Florence Ogilvy Bell was born at 47 Hanover Road, Brondesbury Park, London, the second daughter of Thomas Bell and his wife, Annie Mary Lucas.[4][5] Her father was a photographer and later advertising manager who had been born in Allendale, Northumberland, and later he moved to Greycotes, Ambleside. Florence grew up in London and attended Haberdashers' Aske Girls School in Acton.[4]

Education

Bell studied Natural Sciences at Girton College, Cambridge between 1932-1935, concentrating on chemistry, physics and mineralogy.[6] Whilst a student at Girton College, Cambridge, she was taught how to use x-ray crystallography to study biological molecules by John Desmond Bernal.[6] She moved to the University of Manchester, where she worked with Lawrence Bragg on protein crystallography.

In 1937, William Astbury wrote to Lawrence Bragg looking for a good crystallographer, and he recommended Bell as an "excellent candidate".[6] In 1937 Bell arrived at the University of Leeds, where she joined Astbury's laboratory.[3] During her graduate studies she used X-ray diffraction to characterise biomolecules, including nucleic acids.[7] Her initial work was on the structure of protein multilayers, but after Leeds received samples of highly purified DNA, Astbury directed her to study DNA as the second part of her Ph.D. thesis.[3] She received her Ph.D. in 1939. Her notebook and thesis are held at the University of Leeds Special Collections[8][9]

Career

Astbury's original appointment at the University of Leeds was to study textile physics, where he identified a change in keratin inside wool fibres from alpha to beta form on stretching.[10] In 1939 Bell gave a talk about textiles during an Institute of Physics conference at the University of Leeds, which was covered in the Yorkshire Evening Post in an article entitled "Women Scientist Explains".[6] In the article Bell was described as a "slim University of Cambridge graduate".[6]

DNA crystallography

In 1937, Astbury became interested in DNA and directed Bell to work on the molecule. Bell came up with a method to stretch out the fibers to make dried films of purified DNA, with which she took x-ray diffraction photographs that were clearer than previous work.[3] Her work confirmed it was a regular, ordered structure with periodicity of 3.3 - 3.4 Å along the axis.[6][8] She studied the nucleic acids in yeast, pancreases, tobacco mosaic virus and calf thymus.[7][10] She recognised that the "beginnings of life are clearly associated with the interaction of proteins and nucleic acids".[11] Bell and Astbury published an X-ray study on DNA in 1938, describing the nucleotides as a "Pile of Pennies".[12] Astbury presented their work at the Cold Spring Harbor Laboratory.[13] At the time, they were unaware that DNA can change conformation from A to B-form with humidity, and as a result their photographs are more blurry than the later Photo 51 x-ray image[7] taken by Gosling in 1952.

Astbury and Bell's recent developments on x-ray studies of proteins were included in the conference proceedings, "X-ray and the Stoichiochemistry of Proteins", "An X-ray Study of Thymonucleic Acid" and "Optical and X-ray Examination and Direct Measurement of Built-up Protein Multilayers".[14] Astbury greatly admired Bell's willingness to challenge his ideas, referring to her as his "vox diabolica" (Devil's Advocate).[6][13][15]

World war II and after

In 1941 Bell was enlisted to the Women's Auxiliary Air Force.[6] The University of Leeds and William Astbury fought to get her back to the laboratory, keeping her position on hold and writing to the War Office.[6] But Bell had fallen in love with an American serviceman, Capt. James Herbert Sawyer,[4] and wrote to the University to say she was going to get married and move to the States.[6]

Bell and Sawyer were married 21 December 1942 at St. Mary's Church in Ambleside. She then moved with her husband to the United States where she was employed by the British Air Commission in Washington, D.C. and later she worked as an industrial chemist for the Magnolia Petroleum Company in Beaumont, Texas.[16] She died in Hereford in 2000.

Legacy

The importance of Bell's work on DNA is that, although today we know that several features of her proposed model are incorrect, it nevertheless showed that DNA had a regular, ordered structure that could be studied using X-ray crystallography and so laid the foundations for later work by Maurice Wilkins, Rosalind Franklin and Raymond Gosling, as well as providing James Watson and Francis Crick with a key measurement - the distance between adjacent bases - when they began their own attempt to build a model of DNA.[17] It is also worth noting that this work was done at a time when most scientists believed that proteins were the genetic material and that DNA was just a structural component composed of a monotonous repeat of bases.

Bell's note book is held in the Leeds University archives.[18] She is included in the Oxford Dictionary of National Biography.[19]

gollark: SERIOUSLY?
gollark: It was very boring.
gollark: ?tag edit "lyricly projects" At least mindbreak & macron & petcruel + possibly chess world. Will never be finished.
gollark: <@319753218592866315> What DO you think I did? Feel free to do the 1/20-chance-of-anonymizing-it thing.
gollark: Or LyricLy's foolish and futile attempts to guess what I wrote.

References
  1. England & Wales, Civil Registration Death Index, 1916-2007
  2. U.S., Social Security Applications and Claims Index, 1936-2007
  3. Williams, Gareth (2019). Unravelling the Double Helix. New York: Pegasus Books. pp. 159–162. ISBN 978-1-64313-215-0.
  4. K. T. Butler (1948). Girton College Register, 1869-1946. Cambridge : Privately printed. p. 456.
  5. Oxford dictionary of national biography. British Academy., Oxford University Press. (Online ed.). Oxford. ISBN 9780198614128. OCLC 56568095.CS1 maint: others (link)
  6. "Florence Bell: The Other 'Dark Lady of DNA'? – The British Society for the History of Science (BSHS)". www.bshs.org.uk. Retrieved 2018-04-25.
  7. Ph.D., Fry, Michael (2016-06-10). Landmark experiments in molecular biology. London, UK. ISBN 978-0128021088. OCLC 951807569.
  8. Bell, Florence (1939). "X-ray and related studies of the structure of the proteins and nucleic acids". www.leeds.ac.uk. University of Leeds. Retrieved 2018-04-25. (File).
  9. "Florence Bell's PhD Thesis", Museum of the History of Science, Technology, and Medicine, University of Leeds, 2010. Retrieved 2019-10-20.
  10. Hall, Kersten (2011). "William Astbury and the biological significance of nucleic acids, 1938–1951". Studies in History and Philosophy of Science Part C: Studies in History and Philosophy of Biological and Biomedical Sciences. 42 (2): 119–128. doi:10.1016/j.shpsc.2010.11.018. ISSN 1369-8486. PMID 21486649.
  11. Wainwright, Martin (2010-11-23). "Sidelined scientist who came close to discovering DNA is celebrated at last". the Guardian. Retrieved 2018-04-25.
  12. ASTBURY, W. T.; BELL, FLORENCE O. (1938). "X-Ray Study of Thymonucleic Acid". Nature. 141 (3573): 747–748. doi:10.1038/141747b0. ISSN 0028-0836.
  13. Cecil), Olby, Robert C. (Robert (1994). The path to the double helix : the discovery of DNA. New York: Dover Publications. ISBN 9780486166599. OCLC 608936643.
  14. Astbury, W. T.; Bell, Florence O. (1938-01-01). "Some Recent Developments in the X-Ray Study of Proteins and Related Structures". Cold Spring Harbor Symposia on Quantitative Biology. 6: 109–121. doi:10.1101/SQB.1938.006.01.013. ISSN 0091-7451.
  15. T., Hall, Kersten (2014). The man in the monkeynut coat : William Astbury and the forgotten road to the double-helix (First ed.). Oxford, United Kingdom. ISBN 9780198704591. OCLC 866619867.
  16. Kersten T. Hall (2014). The Man in the Monkeynut Coat: William Astbury and the Forgotten Road to the Double-helix. Oxford University Press. p. 105. ISBN 978-0-19-870459-1.
  17. Watson, James D. (1968). The Double Helix. Weidenfeld & Nicolson. p. 54.
  18. "Laboratory notebook of Florence Ogilvy Bell - Library | University of Leeds". explore.library.leeds.ac.uk. Retrieved 2018-04-27.
  19. Hall, Kersten (2019). "Bell [married name Sawyer], Florence Ogilvy (1913–2000), biophysicist and molecular biologist". Oxford Dictionary of National Biography (online ed.). Oxford University Press. doi:10.1093/odnb/9780198614128.013.369101. Retrieved 2019-08-14. (Subscription or UK public library membership required.)
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