Escherichia virus T4

Escherichia virus T4 is a species of bacteriophages that infect Escherichia coli bacteria. It is a double-stranded DNA virus in the subfamily Tevenvirinae from the family Myoviridae. T4 is capable of undergoing only a lytic lifecycle and not the lysogenic lifecycle. The species was formerly named T-even bacteriophage, a name which also encompasses, among other strains (or isolates), Enterobacteria phage T2, Enterobacteria phage T4 and Enterobacteria phage T6.

Atomic structural model of Bacteriophage T4. By Dr Victor Padilla-Sanchez, PhD (Washington Metropolitan University, Washington DC)
Escherichia virus T4
Virus classification
(unranked): Virus
Realm: Duplodnaviria
Kingdom: Heunggongvirae
Phylum: Uroviricota
Class: Caudoviricetes
Order: Caudovirales
Family: Myoviridae
Genus: Tequatrovirus
Species: Escherichia virus T4
Strains[1]
  • Enterobacteria phage C16
  • Enterobacteria phage F10
  • Enterobacteria phage Fs-alpha
  • Enterobacteria phage PST
  • Enterobacteria phage SKII
  • Enterobacteria phage SKV
  • Enterobacteria phage SKX
  • Enterobacteria phage SV3
  • Enterobacteria phage T2
  • Enterobacteria phage T4
  • Enterobacteria phage T6
Synonyms[2]

Enterobacteria phage T4

Bacteriophage means to "eat bacteria", and phages are well known for being obligate intracellular parasites that reproduce within the host cell and are released when the host is destroyed by lysis. Containing about 160 genes, these virulent viruses are among the largest, most complex viruses that are known and one of the best studied model organisms. They have played a key role in the development of virology and molecular biology.[3][4]

Use in research

Dating back to the 1940s till date, T-even phages are considered the best studied model organisms. Model organisms are usually required to be simple with as few as five genes. Yet, T-even phages are in fact among the largest and highest complexity virus, in which these phages genetic information is made up of around 160 genes. Coincident with their complexity, T-even viruses were found to have an unimaginable feature of no other, the presence of the unusual base hydroxymethylcytosine (HMC) in place of the nucleic acid base cytosine. In addition to this, the HMC residues on the T-even phage are glucosylated in a specific pattern. This unique feature allowed the formation of new enzymes that never existed in T-even infected cells or any other cell and modifying T-even phage DNA provided a basic underlying advancement in viral and molecular levels. Other unique features of the T-even virus is its regulated gene expression.[5] These unique features and other features gave significance of the T-even phages, this includes transduction which is responsible for transfer of drug resistant features, lysogenic conversion is responsible for acquisition of new characteristics such as the formation of new enzymes, random insertion into bacterial chromosome can induce insertional mutation, epidemiological typing of bacteria (phage typing), phages are used extensively in genetic engineering where they serve as cloning vectors. Libraries of genes and monoclonal antibodies are maintained in phages. In addition to all this they are responsible for natural removal of bacteria from water bodies.[6]

Genome and structure

The T4 virus's double-stranded DNA genome is about 169 kbp long[7] and encodes 289 proteins. The T4 genome is terminally redundant and is first replicated as a unit, then several genomic units are recombined end-to-end to form a concatemer. When packaged, the concatemer is cut at unspecific positions of the same length, leading to several genomes that represent circular permutations of the original.[8] The T4 genome bears eukaryote-like intron sequences.

Translation

The Shine-Dalgarno sequence GAGG dominates in virus T4 early genes, whereas the sequence GGAG is a target for the T4 endonuclease RegB that initiates the early mRNA degradation.[9]

Virus particle structure
Structural overview of T2 phage

T4 is a relatively large virus, at approximately 90 nm wide and 200 nm long (most viruses range from 25 to 200 nm in length). The DNA genome is held in an icosahedral head, also known as a capsid.[10] The T4’s tail is hollow so that it can pass its nucleic acid into the cell it is infecting after attachment. Myoviridae phages like T4 have complex contractile tail structures with a large number of proteins involved in the tail assembly and function.[11] The tail fibres are also important in recognizing host cell surface receptors, so they determine if a bacterium is within the virus's host range.[12]

The structure of the 6 megadalton T4 baseplate that comprises 127 polypeptide chains of 13 different proteins (gene products 5, 5.4, 6, 7, 8, 9, 10, 11, 12, 25, 27, 48 and 53) has recently been described in atomic detail. An atomic model of the proximal region of the tail tube formed by gp54 and the main tube protein gp19 have also been created. The tape measure protein gp29 is present in the baseplate-tail tube complexes, but it could not be modeled.[13]

Infection process

The T4 virus initiates an Escherichia coli infection by binding OmpC porin proteins and lipopolysaccharide (LPS) on the surface of E. coli cells with its long tail fibers (LTF).[14][15] A recognition signal is sent through the LTFs to the baseplate. This unravels the short tail fibers (STF) that bind irreversibly to the E. coli cell surface. The baseplate changes conformation and the tail sheath contracts, causing GP5 at the end of the tail tube to puncture the outer membrane of the cell.[16] The lysozyme domain of GP5 is activated and degrades the periplasmic peptidoglycan layer. The remaining part of the membrane is degraded and then DNA from the head of the virus can travel through the tail tube and enter the E. coli cell.

Reproduction

The lytic lifecycle (from entering a bacterium to its destruction) takes approximately 30 minutes (at 37 °C). Virulent bacteriophages multiply in their bacterial host immediately after entry. After the number of progeny phages reach a certain amount, they cause the host to lyse or break down, therefore they would be released and infect new host cells.[17] The process of host lyses and release is called the lytic cycle. Lytic cycle is a cycle of viral reproduction that involves the destruction of the infected cell and its membrane. This cycle involves a virus that overtakes the host cell and its machinery to reproduce. Therefore, the virus must go through 5 stages in order to reproduce and infect the host cell:

After the life cycle is complete, the host cell bursts open and ejects the newly built viruses into the environment, destroying the host cell. T4 has a burst size of approximately 100-150 viral particles per infected host. Complementation, deletion, and recombination tests can be used to map out the rII gene locus by using T4. These Escherichia viruses infect a host cell with their information and then blow up the host cell, thereby propagating themselves.

Adsorption and penetration
Diagram of the DNA injection process

Just like all other viruses, T-even phages don't just randomly attach to the surface of their host; instead they "search" and bind to receptors, specific protein structures, found on the surface of the host. These receptors vary with the phage; teichoic acid, cell wall proteins and lipopolysaccharides, flagella, and pili all can serve as receptors for the phage to bind to. In order for the T-even phage to infect its host and begin its life cycle it must enter the first process of infection, adsorption of the phage to the bacterial cell. Adsorption is a value characteristic of phage-host pair and the adsorption of the phage on host cell surface is illustrated as a 2-stage process: reversible and irreversible. It involves the phages tail structure that begins when the phages tail fibers helps bind the phage to the appropriate receptor of its host. This process is reversible. One or more of the components of the base plate mediates irreversible process of binding of the phage to a bacterium.

Penetration is also a value characteristic of phage-host infection that involves the injection of the phages genetic material inside the bacterium. Penetration of nucleic acid takes place after the irreversible adsorption phase. Mechanisms involving penetration of the phages nucleic acid are specific for each phage. This penetration mechanism can involve electrochemical membrane potential, ATP molecules, enzymatic splitting of peptidoglycan layer, or all three of these factor can be vital for the penetration of the nucleic acid inside the bacterial cell. Studies have been done on the T2 bacteriophage (T4-like phage) mechanism of penetration and it has shown that the phages tail does not penetrate inside the bacterial cell wall and penetration of this phage involves electrochemical membrane potential on the inner membrane.[18][19]

Replication and packaging

Virus T4 genome is synthesized within the host cell using Rolling Circle Replication. The time it takes for DNA replication in a living cell was measured as the rate of virus T4 DNA elongation in virus-infected E. coli.[20] During the period of exponential DNA increase at 37 °C, the rate was 749 nucleotides per second. The mutation rate per base pair per replication during virus T4 DNA synthesis is 1.7 per 10−8,[21] a highly accurate DNA copying mechanism, with only 1 error in 300 copies. The virus also codes for unique DNA repair mechanisms. The T4 Phage head is assembled empty around a scaffolding protein, which is later degraded. Consequently the DNA needs to enter the prohead through a tiny pore, which is achieved by a hexamer of gp17 interactng with DNA first, which also serves as a motor and nuclease. The T4 DNA packaging motor has been found to load DNA into virus capsids at a rate up to 2000 base pairs per second. The power involved, if scaled up in size, would be equivalent to that of an average automobile engine.[22]

Transduction occurs within the lytic cycle where units of the bacterial DNA are packaged in a phage capsid.[23]

Release

The final step in viral reproduction and multiplication is determined by the release of virions from the host cell. The release of the virions occurs after the breakage of the bacterial plasma membrane. Nonenveloped viruses lyse the host cell which is characterized by viral proteins attacking the peptidoglycan or membrane. The lysis of the bacteria occurs when the capsids inside the cell release the enzyme lysozyme which break down the cell wall. The released bacteriophages infect other cells, and the viral multiplication cycle is repeated within those cells.

Lysogenic cycle
Phage2

Lysogenic phages multiply in one of two ways; either by entering an inactive or latent state or by multiplying through the lytic phase. Through a process known as lysogeny, the phage DNA replicates with the replication of the host chromosome by assimilating into the host chromosome itself. It is then passed on to its daughter cells; this is why it usually isn’t recognized by the host. A process known as lysogenic or phage conversion changes the properties of the bacteria cell; this is possible because the prophage itself contains genes that can present new properties into the bacteria cell or host cell. These bacteria are considered lysogenized. Lysogenized bacteria are resistant to superinfection by same or related phages. This is known as superinfection immunity.[24]

Multiplicity reactivation
Survival curves for virus T4 with DNA damaged by UV (top) or MMC (bottom) after single virus T4 infecting host cells (monocomplexes) or two or more virus T4 simultaneously infecting host cells (multicomplexes).

Multiplicity reactivation (MR) is the process by which two or more virus genomes, each containing inactivating genome damage, can interact within an infected cell to form a viable virus genome. Salvador Luria, while studying UV irradiated virus T4 in 1946, discovered MR and proposed that the observed reactivation of damaged virus occurs by a recombination mechanism.(see refs.[25][26][27]) This preceded the confirmation of DNA as the genetic material in 1952 in related virus T2 by the Hershey–Chase experiment.[28]

As remembered by Luria (1984,[29] pg. 97) the discovery of reactivation of irradiated virus (referred to as "multiplicity reactivation") immediately started a flurry of activity in the study of repair of radiation damage within the early phage group (reviewed by Bernstein[30] in 1981). It turned out later that the repair of damaged virus by mutual help that Luria had discovered was only one special case of DNA repair. Cells of all types, not just, bacteria and their viruses, but all organisms studied, including humans, are now known to have complex biochemical processes for repairing DNA damages (see DNA repair). DNA repair processes are also now recognized as playing critical roles in protecting against aging, cancer, and infertility.

MR is usually represented by "survival curves" where survival of plaque forming ability of multiply infected cells (multicomplexes) is plotted against dose of genome damaging agent. For comparison, the survival of virus plaque forming ability of singly infected cells (monocomplexes) is also plotted against dose of genome damaging agent. The top figure shows the survival curves for virus T4 multicomplexes and monocomplexes with increasing dose of UV light. Since survival is plotted on a log scale it is clear that survival of multicomplexes exceeds that of monocomplexes by very large factors (depending on dose). The UV inactivation curve for multicomplexes has an initial shoulder. Other virus T4 DNA damaging agents with shoulders in their multicomplex survival curves are X-rays[31][32] and ethyl methane sulfonate (EMS).[30] The presence of a shoulder has been interpreted to mean that two recombinational processes are used.[33] The first one repairs DNA with high efficiency (in the "shoulder"), but is saturated in its ability as damage increases; the second pathway functions at all levels of damage. Surviving T4 virus released from multicomplexes show no increase in mutation, indicating that MR of UV irradiated virus is an accurate process.[33]

The bottom figure shows the survival curves for inactivation of virus T4 by the DNA damaging agent mitomycin C (MMC). In this case the survival curve for multicomplexes has no initial shoulder, suggesting that only the second recombinational repair process described above is active. The efficiency of repair by this process is indicated by the observation that a dose of MMC that allows survival of only 1 in 1,000 monocomplexes allows survival of about 70% of multicomplexes. Similar multicomplex survival curves (without shoulders) were also obtained for the DNA damaging agents P32 decay, psoralen plus near-UV irradiation (PUVA), N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), methyl methane sulfonate (MMS) and nitrous acid.[30]

Several of the genes found to be necessary for MR in virus T4 proved to be orthologs for genes essential for recombination in prokaryotes, eukaryotes and archaea. This includes, for instance, T4 gene uvsX[34] which specifies a protein that has three-dimensional structural homology to RecA from Escherichia coli and the homologous protein RAD51 in eukaryotes and RadA in archaea. It has been suggested that the efficient and accurate recombinational repair of DNA damages during MR may be analogous to the recombinational repair process that occurs during meiosis in eukaryotes.[35]

History

Bacteriophages were first discovered by the English scientist Frederick Twort in 1915 and Félix d'Hérelle in 1917. In the late 1930s, T.L. Rakieten proposed either a mixture of raw sewerage or a lysate from E.coli infected with raw sewerage to the two researchers Milislav Demerec and Ugo Fano. These two researchers isolated T3, T4, T5, and T6 from E.coli. Also, in 1932, the researcher J.Bronfenbrenner had studied and worked on the T2 phage, at which the T2 phage was isolated from the virus.[36] This isolation was made from a fecal material rather than from sewerage. At any rate, Delbruck was involved in the discovery of the T even phages. His part was naming the bacteriophages into Type 1(T1), Type 2 (T2), Type 3 (T3), etc.

The specific time and place of T4 virus isolation remains unclear, though they were likely found in sewage or fecal material. T4 and similar viruses were described in a paper by Thomas F. Anderson, Max Delbrück, and Milislav Demerec in November 1944.[37]

A number of Nobel Prize winners worked with virus T4 or T4-like viruses including Max Delbrück, Salvador Luria, Alfred Hershey, James D. Watson, and Francis Crick. Other important scientists who worked with virus T4 include Michael Rossmann, Seymour Benzer, Bruce Alberts, Gisela Mosig,[38] Richard Lenski, and James Bull.

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See also

References
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  2. "ICTV Taxonomy history: Escherichia virus T4". International Committee on Taxonomy of Viruses (ICTV). Retrieved 26 December 2018. Caudovirales > Myoviridae > Tevenvirinae > T4virus > Escherichia virus T4
  3. Norkin, Leonard C. (2010). Virology, Molecular Biology and Pathogenesis. Washington: American Society for Microbiology. p. 725. ISBN 978-1-55581-453-3.
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Further reading
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