Caffeate O-methyltransferase
In enzymology, a caffeate O-methyltransferase (EC 2.1.1.68) is an enzyme that catalyzes the chemical reaction
- S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
| caffeate O-methyltransferase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 2.1.1.68 | ||||||||
| CAS number | 50936-45-3 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
Thus, the two substrates of this enzyme are S-adenosyl methionine and 3,4-dihydroxy-trans-cinnamate (caffeic acid), whereas its two products are S-adenosylhomocysteine and 3-methoxy-4-hydroxy-trans-cinnamate (ferulic acid).
This enzyme belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. The systematic name of this enzyme class is S-adenosyl-L-methionine:3,4-dihydroxy-trans-cinnamate 3-O-methyltransferase. Other names in common use include caffeate methyltransferase, caffeate 3-O-methyltransferase, and S-adenosyl-L-methionine:caffeic acid-O-methyltransferase. This enzyme participates in phenylpropanoid biosynthesis.
Structural studies
As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 1KYW and 1KYZ.
References
- Hahlbrock K; Schaller-Hekeler, B; Knobloch, KH; Wellman, E; Grisebach, H; Hahlbrock, K (1974). "Coordinated changes in enzyme activities of phenylpropanoid metabolism during the growth of soybean cell suspension cultures". Biochim. Biophys. Acta. 362 (3): 417–24. doi:10.1016/0304-4165(74)90137-8. PMID 4472044.
- Poulton JE, Butt VS (1975). "Purification and properties of S-adenosyl-L-methionine: caffeic acid O-methyltransferase from leaves of spinach beet (Beta vulgaris L)". Biochim. Biophys. Acta. 403 (2): 301–14. doi:10.1016/0005-2744(75)90060-1. PMID 241400.
- Shimada M, Kuroda H, Higuchi T (1973). "Evidence for the formation of methoxyl groups of ferulic and sinapic acid in Bambusa by the same O-methyltransferase". Phytochemistry. 12 (12): 2873–2875. doi:10.1016/0031-9422(73)80498-4.