Aquacobalamin reductase (NADPH)
Aquacobalamin reductase (NADPH) (EC 1.16.1.5) is an enzyme that catalyzes the chemical reaction
- 2 cob(II)alamin + NADP+ 2 aquacob(III)alamin + NADPH + H+
| aquacobalamin reductase (NADPH) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 1.16.1.5 | ||||||||
| CAS number | 110777-32-7 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
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Thus, the two substrates of this enzyme are cob(II)alamin and nicotinamide adenine dinucleotide phosphate ion, whereas its 3 products are aquacob(III)alamin, nicotinamide adenine dinucleotide phosphate, and hydrogen ion.
This enzyme belongs to the family of oxidoreductases, specifically those oxidizing metal ion with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is cob(II)alamin:NADP+ oxidoreductase. Other names in common use include aquacobalamin (reduced nicotinamide adenine dinucleotide phosphate), reductase, NADPH-linked aquacobalamin reductase, and NADPH2:aquacob(III)alamin oxidoreductase. This enzyme participates in porphyrin and chlorophyll metabolism. It employs one cofactor, FAD.
References
- Watanabe F, Oki Y, Nakano Y, Kitaoka S (1987). "Purification and characterization of aquacobalamin reductase (NADPH) from Euglena gracilis". J. Biol. Chem. 262 (24): 11514–8. PMID 3114247.
- Watanabe F, Yamaji R, Isegawa Y, Yamamoto T, Tamura Y, Nakano Y (1993). "Characterization of aquacobalamin reductase (NADPH) from Euglena gracilis". Arch. Biochem. Biophys. 305 (2): 421–7. doi:10.1006/abbi.1993.1441. PMID 8373179.