23S rRNA (uracil1939-C5)-methyltransferase

23S rRNA (uracil1939-C5)-methyltransferase (EC 2.1.1.190, RumA, RNA uridine methyltransferase A, YgcA) is an enzyme with systematic name S-adenosyl-L-methionine:23S rRNA (uracil1939-C5)-methyltransferase.[1][2][3][4][5][6] This enzyme catalyses the following chemical reaction

S-adenosyl-L-methionine + uracil1939 in 23S rRNA S-adenosyl-L-homocysteine + 5-methyluracil1939 in 23S rRNA
23S rRNA (uracil1939-C5)-methyltransferase
Identifiers
EC number2.1.1.190
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum

The enzyme specifically methylates uracil1939 at C5 in 23S rRNA.

References
  1. Agarwalla S, Kealey JT, Santi DV, Stroud RM (March 2002). "Characterization of the 23 S ribosomal RNA m5U1939 methyltransferase from Escherichia coli". The Journal of Biological Chemistry. 277 (11): 8835–40. doi:10.1074/jbc.M111825200. PMID 11779873.
  2. Lee TT, Agarwalla S, Stroud RM (March 2004). "Crystal structure of RumA, an iron-sulfur cluster containing E. coli ribosomal RNA 5-methyluridine methyltransferase". Structure. 12 (3): 397–407. doi:10.1016/j.str.2004.02.009. PMID 15016356.
  3. Madsen CT, Mengel-Jørgensen J, Kirpekar F, Douthwaite S (August 2003). "Identifying the methyltransferases for m(5)U747 and m(5)U1939 in 23S rRNA using MALDI mass spectrometry". Nucleic Acids Research. 31 (16): 4738–46. doi:10.1093/nar/gkg657. PMC 169892. PMID 12907714.
  4. Persaud C, Lu Y, Vila-Sanjurjo A, Campbell JL, Finley J, O'Connor M (February 2010). "Mutagenesis of the modified bases, m(5)U1939 and psi2504, in Escherichia coli 23S rRNA". Biochemical and Biophysical Research Communications. 392 (2): 223–7. doi:10.1016/j.bbrc.2010.01.021. PMID 20067766.
  5. Agarwalla S, Stroud RM, Gaffney BJ (August 2004). "Redox reactions of the iron-sulfur cluster in a ribosomal RNA methyltransferase, RumA: optical and EPR studies". The Journal of Biological Chemistry. 279 (33): 34123–9. doi:10.1074/jbc.M405702200. PMC 1237038. PMID 15181002.
  6. Lee TT, Agarwalla S, Stroud RM (March 2005). "A unique RNA Fold in the RumA-RNA-cofactor ternary complex contributes to substrate selectivity and enzymatic function". Cell. 120 (5): 599–611. doi:10.1016/j.cell.2004.12.037. PMID 15766524.
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