2-methylcitrate dehydratase
In enzymology, a 2-methylcitrate dehydratase (EC 4.2.1.79) is an enzyme that catalyzes the chemical reaction
- (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate (Z)-but-2-ene-1,2,3-tricarboxylate + H2O
| 2-methylcitrate dehydratase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 4.2.1.79 | ||||||||
| CAS number | 80891-26-5 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
Hence, this enzyme has one substrate, (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate, and two products, (Z)-but-2-ene-1,2,3-tricarboxylate and H2O.
This enzyme belongs to the family of lyases, specifically the hydro-lyases, which cleave carbon-oxygen bonds. The systematic name of this enzyme class is (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate hydro-lyase [(Z)-but-2-ene-1,2,3-tricarboxylate-forming]. Other names in common use include 2-methylcitrate hydro-lyase, PrpD, and 2-hydroxybutane-1,2,3-tricarboxylate hydro-lyase. This enzyme participates in propanoate metabolism.
Structural studies
As of late 2007, only one structure has been solved for this class of enzymes, with the PDB accession code 1SZQ.
References
- Aoki H, Tabuchi T (1981). "Purification and properties of 2-methylcitrate dehydratase from Yarrowia lipolytica". Agric. Biol. Chem. 45 (12): 2831–2837. doi:10.1271/bbb1961.45.2831.
- Brock M, Maerker C, Schutz A, Volker U, Buckel W (2002). "Oxidation of propionate to pyruvate in Escherichia coli. Involvement of methylcitrate dehydratase and aconitase". Eur. J. Biochem. 269 (24): 6184–94. doi:10.1046/j.1432-1033.2002.03336.x. PMID 12473114.