2-methylcitrate dehydratase

In enzymology, a 2-methylcitrate dehydratase (EC 4.2.1.79) is an enzyme that catalyzes the chemical reaction

(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate (Z)-but-2-ene-1,2,3-tricarboxylate + H2O
2-methylcitrate dehydratase
Identifiers
EC number4.2.1.79
CAS number80891-26-5
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO

Hence, this enzyme has one substrate, (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate, and two products, (Z)-but-2-ene-1,2,3-tricarboxylate and H2O.

This enzyme belongs to the family of lyases, specifically the hydro-lyases, which cleave carbon-oxygen bonds. The systematic name of this enzyme class is (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate hydro-lyase [(Z)-but-2-ene-1,2,3-tricarboxylate-forming]. Other names in common use include 2-methylcitrate hydro-lyase, PrpD, and 2-hydroxybutane-1,2,3-tricarboxylate hydro-lyase. This enzyme participates in propanoate metabolism.

Structural studies

As of late 2007, only one structure has been solved for this class of enzymes, with the PDB accession code 1SZQ.

gollark: Exactly.
gollark: Store literally no state whatsoever in memory ever.
gollark: Well, just write stateless code?!?!?!?!?
gollark: I have osmarksßservers for long-running jobs like that.
gollark: Oh no, imagine computers not constantly saving all state?!?!?!

References

    • Aoki H, Tabuchi T (1981). "Purification and properties of 2-methylcitrate dehydratase from Yarrowia lipolytica". Agric. Biol. Chem. 45 (12): 2831–2837. doi:10.1271/bbb1961.45.2831.
    • Brock M, Maerker C, Schutz A, Volker U, Buckel W (2002). "Oxidation of propionate to pyruvate in Escherichia coli. Involvement of methylcitrate dehydratase and aconitase". Eur. J. Biochem. 269 (24): 6184–94. doi:10.1046/j.1432-1033.2002.03336.x. PMID 12473114.


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