Unsettling Gender Reveal/Quotes

Quagmire: Hey there little lady. Why don't you turn around and show me your lower east side.

"Lady": (in masculine voice) Sure.

Quagmire: Whoa, transvestite! Back off! Wait a minute, pre-op or post-op?

"Lady": Pre-op.

Quagmire: Whoa, transvestite! Back off!

—Family Guy

Bridget: Hey, what's wrong with you? You suddenly went limp right in the middle of the fight...
Johnny: Ahh... Johnny... What have you done... You... you're a guy, aren't you!

Bridget: Sure am. This appearance... Well, it's kind of a long story.

Johnny: I can't believe I just tried to seduce another man...

—Johnny of Guilty Gear XX, after learning the truth about the former Trope Namer's gender

Wait, Bridget is a dude?
Oh god.
Oh God!

OH GOD!

—New Anonymous that needs to lurk moar.

So I was looking up Shion's story... until I found out "she" was a "he"... GODDAMN YOU SNK AND YOUR POINTLESS SQUAREENIX-STYLE GENDER BENDING!!!

—zigg4d, a MUGEN user after a Bridget was dropped on him

Jerry: I'm a man!

Osgood: Well, nobody's perfect.

—Some Like It Hot, final lines of dialogue

Oh, sweet Jesus, she's got a penis.

—Mark Henry, WWE Monday Night RAW

Fascinating creatures. Look like women, but they have... boy parts. I'm not attracted to them! They're just... confusing.

—Alan Partridge on Ladyboys, I'm Alan Partridge

Peter: If you could be stranded on a desert island with any woman in the world, who would it be?
Quagmire: Taylor Hanson.
Joe: Taylor Hanson is a guy.
Quagmire: (laughs) You guys are yankin' me. "Hey, let's put one over on Quagmire."
Peter: No, he's actually a guy, Quagmire.

Quagmire: ...What? That's insane. That's impossible. ...Oh god. Oh my god. I've got all these magazines! Oh god!

—Family Guy

If it's pretty, f*** it. There is no gay.

—Courage Wolf

...Where are your boobs!?

—Hinoe, Natsume Yuujinchou

I told you before...I am not a woman! I'm a man!

—Nando, Pokémon XD3: The Waves Of Truth

Hal: Hey baby, what's your name?
Birdo: (in deep manly voice) My name is Susan.
Hal: Ahhhhhhh!

(Susan blows a kiss at him.)

—Bowser's Kingdom episode 6