ZWINT

ZW10 interactor (Zwint-1) is a protein that in humans is encoded by the ZWINT gene.[5]

ZWINT
Identifiers
Aliases	ZWINT, HZwint-1, KNTC2AP, ZWINT1, SIP30, ZW10 interacting kinetochore protein
External IDs	OMIM: 609177 MGI: 1289227 HomoloGene: 48496 GeneCards: ZWINT
Gene location (Human) Chr. Chromosome 10 (human)[1] Band 10q21.1 Start 56,357,227 bp[1] End 56,361,273 bp[1]
Gene location (Mouse) Chr. Chromosome 10 (mouse)[2] Band 10 B5.3|10 37.15 cM Start 72,654,845 bp[2] End 72,674,964 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • GO:0001948 protein binding • protein N-terminus binding Cellular component • cytoplasm • condensed chromosome kinetochore • neuron projection • dendrite • chromosome, centromeric region • intracellular membrane-bounded organelle • kinetochore • chromosome • cell nucleus • nucleoplasm • cytosol • nuclear body Biological process • mitotic sister chromatid segregation • mitotic cell cycle checkpoint • cell division • cell cycle • establishment of localization in cell Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	11130	52696
Ensembl	ENSG00000122952	ENSMUSG00000019923
UniProt	O95229	Q9CQU5
RefSeq (mRNA)	NM_001005413 NM_001005414 NM_007057 NM_032997	NM_001293683 NM_001293684 NM_025635
RefSeq (protein)	NP_001005413 NP_008988 NP_127490	NP_001280612 NP_001280613 NP_079911
Location (UCSC)	Chr 10: 56.36 – 56.36 Mb	Chr 10: 72.65 – 72.67 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Function

Zwint-1 is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The encoded protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[5]

Interactions

ZWINT has been shown to interact with MIS12.[6][7] ZWINT has also been shown to interact with RAB3C[8]

References

1. GRCh38: Ensembl release 89: ENSG00000122952 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000019923 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. "Entrez Gene: ZWINT ZW10 interactor".
6. Cheeseman IM, Niessen S, Anderson S, Hyndman F, Yates JR, Oegema K, Desai A (Sep 2004). "A conserved protein network controls assembly of the outer kinetochore and its ability to sustain tension". Genes & Development. 18 (18): 2255–68. doi:10.1101/gad.1234104. PMC 517519. PMID 15371340.
7. Obuse C, Iwasaki O, Kiyomitsu T, Goshima G, Toyoda Y, Yanagida M (Nov 2004). "A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1". Nature Cell Biology. 6 (11): 1135–41. doi:10.1038/ncb1187. PMID 15502821.
8. van Vlijmen T, Vleugel M, Evers M, Mohammed S, Wulf PS, Heck AJ, Hoogenraad CC, van der Sluijs P (Aug 2008). "A unique residue in rab3c determines the interaction with novel binding protein Zwint-1". FEBS Letters. 582 (19): 2838–42. doi:10.1016/j.febslet.2008.07.012. PMID 18625232.

Further reading

• Maruyama K, Sugano S (Jan 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Bonaldo MF, Lennon G, Soares MB (Sep 1996). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Research. 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (Oct 1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Starr DA, Saffery R, Li Z, Simpson AE, Choo KH, Yen TJ, Goldberg ML (Jun 2000). "HZwint-1, a novel human kinetochore component that interacts with HZW10". Journal of Cell Science. 113 ( Pt 11) (11): 1939–50. PMID 10806105.
• Musio A, Mariani T, Montagna C, Zambroni D, Ascoli C, Ried T, Vezzoni P (Apr 2004). "Recapitulation of the Roberts syndrome cellular phenotype by inhibition of INCENP, ZWINT-1 and ZW10 genes". Gene. 331: 33–40. doi:10.1016/j.gene.2004.01.028. PMID 15094189.
• Cheeseman IM, Niessen S, Anderson S, Hyndman F, Yates JR, Oegema K, Desai A (Sep 2004). "A conserved protein network controls assembly of the outer kinetochore and its ability to sustain tension". Genes & Development. 18 (18): 2255–68. doi:10.1101/gad.1234104. PMC 517519. PMID 15371340.
• Wang H, Hu X, Ding X, Dou Z, Yang Z, Shaw AW, Teng M, Cleveland DW, Goldberg ML, Niu L, Yao X (Dec 2004). "Human Zwint-1 specifies localization of Zeste White 10 to kinetochores and is essential for mitotic checkpoint signaling". The Journal of Biological Chemistry. 279 (52): 54590–8. doi:10.1074/jbc.M407588200. PMID 15485811.
• Obuse C, Iwasaki O, Kiyomitsu T, Goshima G, Toyoda Y, Yanagida M (Nov 2004). "A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1". Nature Cell Biology. 6 (11): 1135–41. doi:10.1038/ncb1187. PMID 15502821.
• Le XF, Lammayot A, Gold D, Lu Y, Mao W, Chang T, Patel A, Mills GB, Bast RC (Jan 2005). "Genes affecting the cell cycle, growth, maintenance, and drug sensitivity are preferentially regulated by anti-HER2 antibody through phosphatidylinositol 3-kinase-AKT signaling". The Journal of Biological Chemistry. 280 (3): 2092–104. doi:10.1074/jbc.M403080200. PMID 15504738.
• Kops GJ, Kim Y, Weaver BA, Mao Y, McLeod I, Yates JR, Tagaya M, Cleveland DW (Apr 2005). "ZW10 links mitotic checkpoint signaling to the structural kinetochore". The Journal of Cell Biology. 169 (1): 49–60. doi:10.1083/jcb.200411118. PMC 1351127. PMID 15824131.
• Lin YT, Chen Y, Wu G, Lee WH (Nov 2006). "Hec1 sequentially recruits Zwint-1 and ZW10 to kinetochores for faithful chromosome segregation and spindle checkpoint control". Oncogene. 25 (52): 6901–14. doi:10.1038/sj.onc.1209687. PMID 16732327.

External links

• ZWINT human gene location in the UCSC Genome Browser.
• ZWINT human gene details in the UCSC Genome Browser.