Tryptic soy broth

Tryptic soy broth or Trypticase soy broth (frequently abbreviated as TSB) is used in microbiology laboratories as a culture broth to grow aerobic bacteria. It is a complex, general purpose medium that is routinely used to grow certain pathogenic bacteria, which tend to have high nutritional requirements (i.e., they are fastidious). Its agar counterpart is tryptic soy agar (TSA).[1] One of the components of Tryptic soy broth is Phytone, which is an enzymatic digest of soybean meal.

TSB is frequently used in commercial diagnostics in conjunction with the additive sodium thioglycolate which promotes growth of anaerobes.[2]

Preparation

To prepare TSB, the following ingredients are dissolved under gentle heat and then autoclaved for 15 minutes at 121 °C (250 °F).

[3]

gollark: Would that work? How is Jesus's water-walking thing implemented?
gollark: You can check whether the results of it are good by some other metric, but that just pushes the problem up a level.
gollark: Regarding objective morality: I don't understand how it's meant to work. Generally we consider things "true" if they're well-established by experiment and observation. I do not see how you can empirically test whether something is what you "should" do.
gollark: A kilobee is 1000 bees.
gollark: Not really. I meant that the arguments roger was making skip a lot of steps through equivocation things.

References
  1. http://www.rci.rutgers.edu/~microlab/CLASSINFO/IMAGESCI/Culture%20medium%20introduction.htm
  2. Doyle, JE; Mehrhof, WH; Ernst, RR (1968). "Limitations of thioglycolate broth as a sterility test medium for materials exposed to gaseous ethylene oxide". Appl Microbiol. 16 (11): 1742–4. PMC 547751. PMID 4973064.
  3. "BAM Media M154: Trypticase (Tryptic) Soy Broth". Food and Drug Administration. January 2001. Retrieved 18 April 2015.


This article is issued from Wikipedia. The text is licensed under Creative Commons - Attribution - Sharealike. Additional terms may apply for the media files.