M17 agar

This bacterial growth medium was developed in 1971 for Lactococcus species isolated from milk products. It was originally called M16 medium,[1] but in 1975 Terzaghi and Sandine[2] added disodium-β-glycerophosphate to the medium as a buffer, and named the new growth medium M17 medium. It was later found that the addition of disodium-β-glycerophosphate inhibits the growth of many Lactobacillus species.[3]

Typical composition

Per 950 mL:[2]

Preparation:

  • 1. Heat with frequent agitation and boil for 1 minute to completely dissolve.
  • 2. Autoclave at 121 °C for 15 minutes. Cool to 50 °C.
  • 3. Add 50 ml filter sterilized 10% lactose solution and mix well (the lactose can be exchanged to other carbohydrates e.g. glucose, resulting in GM17 medium)
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gollark: Probably 230 or so?
gollark: At least 3.
gollark: Hopefully I'll get some when I'm at my computer and fast e-webbernet.
gollark: *still unable to catch any holidays*

References
  1. Lowrie RJ, Pearce LE. (1971). "The plating efficiency of bacteriophages of lactic streptococci". N.Z. J. Dairy Sci. Technol. 6: 166–171.CS1 maint: uses authors parameter (link)
  2. Terzaghi BE, Sandine WE. (1975). "Improved medium for lactic streptococci and their bacteriophages". Applied Microbiology. 29 (6): 807–813. PMC 187084. PMID 16350018.CS1 maint: uses authors parameter (link)
  3. Shankar PA, Davies FL. (1977). "A note on the suppression of Lactobacillus bulgaricus in media containing β-glycerophosphate and application of the media to selective isolation of Streptococcus thermophilus from yoghurt". J. Soc. Dairy Technol. 30 (1): 28–30. doi:10.1111/j.1471-0307.1977.tb01162.x.CS1 maint: uses authors parameter (link)
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