Spot-tag

A Spot-tag is a 12-amino acid peptide tag recognized by a (single-domain antibody nanobody), (sdAb). Due to the small size of a Spot-tag (12 amino acids) and the robust Spot-nanobody (14.7 kD) that specifically binds to Spot-tagged proteins, Spot-tag can be used for multiple capture and detection applications: Immunoprecipitation, affinity purification, immunofluorescence, and super-resolution microscopy.[1] Recombinant proteins can be engineered to express the Spot-tag.

Spot-tag Sequence

Amino acid sequence

PDRVRAVSHWSS

Codon optimized DNA sequence

Human cell line CCA GAC CGC GTG CGC GCC GTG AGC CAT TGG AGC AGC

S. cerevisiae CCA GAT AGA GTT AGA GCT GTT TCT CAT TGG TCT TCT

E. coli CCG GAT CGC GTG CGC GCA GTC TCT CAC TGG AGC AGC

gollark: States set their own laws in some things, the central government sets laws for other things.
gollark: I have a rough idea.
gollark: Which is ironic given that it was originally designed to not do much.
gollark: The federal government does a lot, so I think there's decent consistency in *laws*.
gollark: I already said that. You are reusing my jokes. CEASE.

See also

References
  1. Virant D, Traenkle B, Maier J, Kaiser PD, Bodenhöfer M, Schmees C, Pisak-Lukáts B, Endesfelder U, Rothbauer U (2018). "A peptide tag-specific nanobody enables high-quality labeling for STORM imaging". Nature Communications. 9 (1): 930. Bibcode:2018NatCo...9..930V. doi:10.1038/s41467-018-03191-2. PMC 5834503. PMID 29500346.


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