R2A agar

R2A agar (Reasoner's 2A agar) is a culture medium[1] developed to study bacteria which normally inhabit potable water.[2] These bacteria tend to be slow-growing species and would quickly be suppressed by faster-growing species on a richer culture medium.

Since its development in 1985,[3] it has been found to allow the culturing of many other bacteria that will not readily grow on fuller, complex organic media.

Typical composition (% w/v)

Proteose peptone, 0.05%
Casamino acids, 0.05%
Yeast extract, 0.05%
Dextrose, 0.05%
Soluble starch, 0.05%
Dipotassium phosphate, 0.03%
Magnesium sulfate, 0.005%
Sodium pyruvate, 0.03%
Agar, 1.5%

Final pH 7 ± 0.2 @ 25 °C

gollark: But this is not accurate. It assumes the only options are "no god" or "basically Christian god".

gollark: Pascal's Wager basically goes "if no god, belief doesn't have costs anyway (wrong, since it takes time and may make your thinking more irrational); if god, non-belief means infinite badness (hell), belief means infinite goodness (heaven), so rationally you should believe".

gollark: There *may* be a god of some kind who rewards you for believing in them and their afterlife and such, but there is an infinity of possible gods including ones like "allocates you to heaven or hell entirely at random", "entirely indistinguishable from no god", "sends you to hell if you believe in the *other* god", "incomprehensible eldritch abomination" or "literal bees".

gollark: PASACL'S WAGER BAD

gollark: ÆÆÆÆÆÆÆÆÆÆÆÆÆÆÆÆÆÆÆÆÆÆÆÆÆÆÆA

References

van der Linde K, Lim BT, Rondeel JM, Antonissen LP, de Jong GM (October 1999). "Improved bacteriological surveillance of haemodialysis fluids: a comparison between Tryptic soy agar and Reasoner's 2A media". Nephrol. Dial. Transplant. 14 (10): 2433–7. doi:10.1093/ndt/14.10.2433. PMID 10528669.
Sandle, T. (July 2004). "An approach for the reporting of microbiological results from water systems". PDA J Pharm Sci Technol. 58 (4): 231–7. PMID 15368993.
Reasoner DJ, EE Geldreich EE (Jan 1985). "A new medium for the enumeration and subculture of bacteria from potable water". Appl. Environ. Microbiol. 49 (1): 1–7. PMC 238333. PMID 3883894.

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[pmid10528669-1] van der Linde K, Lim BT, Rondeel JM, Antonissen LP, de Jong GM (October 1999). "Improved bacteriological surveillance of haemodialysis fluids: a comparison between Tryptic soy agar and Reasoner's 2A media". Nephrol. Dial. Transplant. 14 (10): 2433–7. doi:10.1093/ndt/14.10.2433. PMID 10528669.

[2] Sandle, T. (July 2004). "An approach for the reporting of microbiological results from water systems". PDA J Pharm Sci Technol. 58 (4): 231–7. PMID 15368993.

[pmid42349-3] Reasoner DJ, EE Geldreich EE (Jan 1985). "A new medium for the enumeration and subculture of bacteria from potable water". Appl. Environ. Microbiol. 49 (1): 1–7. PMC 238333. PMID 3883894.