PUM2

Pumilio homolog 2 is an RNA-binding protein that in humans is encoded by the PUM2 gene.[5][6][7][8]

PUM2
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 3Q0Q, 3Q0R, 3Q0S
Identifiers
Aliases	PUM2, PUMH2, PUML2, pumilio RNA binding family member 2
External IDs	OMIM: 607205 MGI: 1931751 HomoloGene: 69183 GeneCards: PUM2
Gene location (Human) Chr. Chromosome 2 (human)[1] Band 2p24.1 Start 20,248,691 bp[1] End 20,352,234 bp[1]
Gene location (Mouse) Chr. Chromosome 12 (mouse)[2] Band 12|12 A1.1 Start 8,674,134 bp[2] End 8,752,581 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • GO:0001948 protein binding • RNA binding • mRNA 3'-UTR binding • miRNA binding Cellular component • perinuclear region of cytoplasm • cytoplasmic stress granule • nuclear membrane • cytoplasm • cytosol Biological process • regulation of chromosome segregation • regulation of gene silencing by miRNA • positive regulation of RIG-I signaling pathway • regulation of translation • regulation of mRNA stability • positive regulation of gene silencing by miRNA • stress granule assembly • posttranscriptional regulation of gene expression • production of miRNAs involved in gene silencing by miRNA Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	23369	80913
Ensembl	ENSG00000055917	ENSMUSG00000020594
UniProt	Q8TB72	Q80U58
RefSeq (mRNA)	NM_001282752 NM_001282790 NM_001282791 NM_015317 NM_001352917 NM_001352918 NM_001352919 NM_001352920 NM_001352923 NM_001352925 NM_001352926 NM_001352927 NM_001352928 NM_001352929 NM_001352930	NM_001160219 NM_001160220 NM_001160221 NM_001160222 NM_030723 NM_001310519
RefSeq (protein)	NP_001269681 NP_001269719 NP_001269720 NP_056132 NP_001339846 NP_001339847 NP_001339848 NP_001339849 NP_001339852 NP_001339854 NP_001339855 NP_001339856 NP_001339857 NP_001339858 NP_001339859	NP_001153691 NP_001153692 NP_001153693 NP_001153694 NP_001297448 NP_109648
Location (UCSC)	Chr 2: 20.25 – 20.35 Mb	Chr 12: 8.67 – 8.75 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Interactions

PUM2 has been shown to interact with the following proteins:

• CPEB[9]
• DAZL[10]
• DAZ1[11]
• NANOS1[12]

References

1. GRCh38: Ensembl release 89: ENSG00000055917 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000020594 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Nagase T, Seki N, Ishikawa K, Ohira M, Kawarabayasi Y, Ohara O, Tanaka A, Kotani H, Miyajima N, Nomura N (May 1997). "Prediction of the coding sequences of unidentified human genes. VI. The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain". DNA Res. 3 (5): 321–9, 341–54. doi:10.1093/dnares/3.5.321. PMID 9039502.
6. Spassov DS, Jurecic R (Dec 2002). "Cloning and comparative sequence analysis of PUM1 and PUM2 genes, human members of the Pumilio family of RNA-binding proteins". Gene. 299 (1–2): 195–204. doi:10.1016/S0378-1119(02)01060-0. PMID 12459267.
7. Moore FL, Jaruzelska J, Fox MS, Urano J, Firpo MT, Turek PJ, Dorfman DM, Pera RA (Jan 2003). "Human Pumilio-2 is expressed in embryonic stem cells and germ cells and interacts with DAZ (Deleted in AZoospermia) and DAZ-Like proteins". Proc Natl Acad Sci U S A. 100 (2): 538–43. doi:10.1073/pnas.0234478100. PMC 141031. PMID 12511597.
8. "Entrez Gene: PUM2 pumilio homolog 2 (Drosophila)".
9. Campbell ZT, Menichelli E, Friend K, Wu J, Kimble J, Williamson JR, Wickens M (2012). "Identification of a conserved interface between PUF and CPEB proteins". J Biol Chem. 287 (22): 18854–62. doi:10.1074/jbc.M112.352815. PMC 3365739. PMID 22496444.
10. Ota R, Kotani T, Yamashita M (2011). "Biochemical characterization of Pumilio1 and Pumilio2 in Xenopus oocytes". J Biol Chem. 286 (4): 2853–63. doi:10.1074/jbc.M110.155523. PMC 3024781. PMID 21098481.
11. Haston KM, Tung JY, Reijo Pera RA (2009). "Dazl functions in maintenance of pluripotency and genetic and epigenetic programs of differentiation in mouse primordial germ cells in vivo and in vitro". PLOS ONE. 4 (5): e5654. doi:10.1371/journal.pone.0005654. PMC 2681483. PMID 19468308.
12. Geyer CB, Saba R, Kato Y, Anderson AJ, Chappell VK, Saga Y, Eddy EM (2012). "Rhox13 is translated in premeiotic germ cells in male and female mice and is regulated by NANOS2 in the male". Biol Reprod. 86 (4): 127. doi:10.1095/biolreprod.111.094938. PMC 3338663. PMID 22190708.

Further reading

• Nakajima D, Okazaki N, Yamakawa H, Kikuno R, Ohara O, Nagase T (2003). "Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones". DNA Res. 9 (3): 99–106. doi:10.1093/dnares/9.3.99. PMID 12168954.
• Spik A, Oczkowski S, Olszak A, Kotecki M, Formanowicz P, Błazewicz J, Jaruzelska J (2006). "Candidate mRNAs interacting with fertility protein PUMILIO2 in the human germ line". Reproductive biology. 6 Suppl 1: 37–42. PMID 16967088.
• Nakayama M, Kikuno R, Ohara O (2003). "Protein–Protein Interactions Between Large Proteins: Two-Hybrid Screening Using a Functionally Classified Library Composed of Long cDNAs". Genome Res. 12 (11): 1773–84. doi:10.1101/gr.406902. PMC 187542. PMID 12421765.
• Jaruzelska J, Kotecki M, Kusz K, Spik A, Firpo M, Reijo Pera RA (2004). "Conservation of a Pumilio-Nanos complex from Drosophila germ plasm to human germ cells". Dev. Genes Evol. 213 (3): 120–6. doi:10.1007/s00427-003-0303-2. PMID 12690449.
• Dazard JE, Gal H, Amariglio N, Rechavi G, Domany E, Givol D (2003). "Genome-wide comparison of human keratinocyte and squamous cell carcinoma responses to UVB irradiation: implications for skin and epithelial cancer". Oncogene. 22 (19): 2993–3006. doi:10.1038/sj.onc.1206537. PMID 12771951.
• Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
• Fox M, Urano J, Reijo Pera RA (2005). "Identification and characterization of RNA sequences to which human PUMILIO-2 (PUM2) and deleted in Azoospermia-like (DAZL) bind". Genomics. 85 (1): 92–105. doi:10.1016/j.ygeno.2004.10.003. PMID 15607425.
• Scott RE, White-Grindley E, Ruley HE, Chesler EJ, Williams RW (2005). "P2P-R expression is genetically coregulated with components of the translation machinery and with PUM2, a translational repressor that associates with the P2P-R mRNA". J. Cell. Physiol. 204 (1): 99–105. doi:10.1002/jcp.20263. PMID 15617101.
• Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983.
• Kusz K, Ginter-Matuszewska B, Ziolkowska K, Spik A, Bierla J, Jedrzejczak P, Latos-Bielenska A, Pawelczyk L, Jaruzelska J (2007). "Polymorphisms of the human PUMILIO2 gene and male sterility". Mol. Reprod. Dev. 74 (6): 795–9. doi:10.1002/mrd.20683. PMID 17154300.