Cystovirus

Cystovirus is a genus of viruses, in the family Cystoviridae.[1] Pseudomonas syringae pathovar phaseolicola bacteria serve as natural hosts. There are currently seven species in this genus, including the type species Pseudomonas virus phi6.[1][2][3]

Cystovirus
The RNA-packaged procapsid (protein shell) of Pseudomonas virus phi6
Virus classification
(unranked): Virus
Realm: Riboviria
Kingdom: Orthornavirae
Phylum: Duplornaviricota
Class: Vidaverviricetes
Order: Mindivirales
Family: Cystoviridae
Genus: Cystovirus
Species
  • Pseudomonas virus phi6
  • Pseudomonas virus phi8
  • Pseudomonas virus phi12
  • Pseudomonas virus phi13
  • Pseudomonas virus phi2954
  • Pseudomonas virus phiNN
  • Pseudomonas virus phiYY

Taxonomy

Group: dsRNA

[1]

Characteristics

Cystoviruses[1] are distinguished by their tripartite dsRNA genome, totaling ~14 kb in length and their protein and lipid outer layer. No other bacteriophage has any lipid in its outer coat, though the Tectiviridae and the Corticoviridae have lipids within their capsids.

Most identified cystoviruses infect Pseudomonas species, but this is likely biased due to the method of screening and enrichment.[4] The type species is Pseudomonas virus phi6, but there are many other proposed members of this family. Pseudomonas viruses φ7, φ8, φ9, φ10, φ11, φ12, and φ13 have been identified and named,[5] but other cystovirus-like viruses have also been isolated.[4] These seven putative relatives are classified as either close (φ7, φ9, φ10, φ11) or distant (φ8, φ12, φ13) relatives to φ6,[5] with the distant relatives thought to infect via the LPS rather than the pili.[6]

Members of the Cystoviridae appear to be most closely related to the Reoviridae,[7] but also share homology with the Totiviridae. In particular, the structural genes of cystoviruses are highly-similar to those used by a number of dsRNA viruses that infect eukaryotes.[8]

Structure

Viruses included in the genus Cystovirus are enveloped, with icosahedral and Spherical geometries, and T=13, T=2 symmetry. The diameter is around 85 nm. Genomes are linear and segmented, and labeled as large (L) 6.4kb, Medium (M) 4 kb and Small (S) 2.9 kb in length. The genome codes for 12 proteins.[1][2]

GenusStructureSymmetryCapsidGenomic arrangementGenomic segmentation
CystovirusIcosahedralT=13 laevo, T=2EnvelopedLinearSegmented

Life cycle

Viral replication is cytoplasmic. Replication follows the double-stranded RNA virus replication model. Double-stranded rna virus transcription is the method of transcription. Pseudomonas bacteria serve as the natural host.[1][2]

GenusHost detailsTissue tropismEntry detailsRelease detailsReplication siteAssembly siteTransmission
CystovirusBacteria: PseudomonasNonePilus adsorption; membrane fusionCell division; sporogenesis; cell fusionCytoplasmCytoplasmUnknown

Discovery

Pseudomonas virus phi6 was the first virus in this family to be discovered and was initially characterized in 1973 by Anne K. Vidaver in the Department of Plant Pathology at the University of Nebraska. She found that when she cultured the bacterial strain Pseudomonas phaseolicola HB1OY with halo blight infected bean straw, cytopathic effects were detected in cultured lawns, indicating that there was a lytic microbe or bacteriophage present.[9]

In 1999, phi7-14 were identified by the laboratory of Leonard Mindich in the Department of Microbiology at the Public Health Research Institute associated with New York University. They did this by culturing various leaves in Lysogeny Broth and then plating the broth on lawns of Pseudomonas syringae pv phaseolicola. They were able to identify viral plaques from this and then subsequently sequence their genomes.[5]

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References
  1. "ICTV Report Cystoviridae".
  2. "Viral Zone". ExPASy. Retrieved 15 June 2015.
  3. "NCBI Taxonomy Browser: Cystoviridae". NCBI. Retrieved 19 June 2016.
  4. Silander OK, Weinreich DM, Wright KM, et al. (December 2005). "Widespread genetic exchange among terrestrial bacteriophages". Proc. Natl. Acad. Sci. U.S.A. 102 (52): 19009–14. Bibcode:2005PNAS..10219009S. doi:10.1073/pnas.0503074102. PMC 1323146. PMID 16365305.
  5. Mindich L, Qiao X, Qiao J, Onodera S, Romantschuk M, Hoogstraten D (August 1999). "Isolation of additional bacteriophages with genomes of segmented double-stranded RNA". J. Bacteriol. 181 (15): 4505–8. PMC 103579. PMID 10419946.
  6. Gottlieb P, Potgieter C, Wei H, and Toporovsky I (2002). "Characterization of φ12, a Bacteriophage Related to φ6: Nucleotide Sequence of the Large Double-Stranded RNA". Virology. 295 (2): 266–271. doi:10.1006/viro.2002.1436. PMID 12033785.
  7. Butcher SJ, Dokland T, Ojala PM, Bamford DH, Fuller SD (July 1997). "Intermediates in the assembly pathway of the double-stranded RNA virus phi6". EMBO J. 16 (14): 4477–87. doi:10.1093/emboj/16.14.4477. PMC 1170074. PMID 9250692.
  8. Koonin, Eugene V.; Dolja, Valerian V.; Krupovic, Mart (2015). "Origins and evolution of viruses of eukaryotes: The ultimate modularity". Virology. 479-480: 2–25. doi:10.1016/j.virol.2015.02.039. PMC 5898234. PMID 25771806.
  9. Vidaver AK, Koski RK, Van Etten JL (May 1973). "Bacteriophage Φ6 a Lipid-Containing Virus of Pseudomonas phaseolicola". Journal of Virology. 11 (15): 799–805.
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