GOLGA2

Golgin subfamily A member 2 is a protein that in humans is encoded by the GOLGA2 gene.[5]

GOLGA2
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 4REY
Identifiers
Aliases	GOLGA2, GM130, golgin A2
External IDs	OMIM: 602580 MGI: 2139395 HomoloGene: 3300 GeneCards: GOLGA2
Gene location (Human) Chr. Chromosome 9 (human)[1] Band 9q34.11 Start 128,255,829 bp[1] End 128,275,995 bp[1]
Gene location (Mouse) Chr. Chromosome 2 (mouse)[2] Band 2|2 B Start 32,287,384 bp[2] End 32,307,921 bp[2]
RNA expression pattern More reference expression data
Gene ontology Molecular function • microtubule binding • importin-alpha family protein binding • GO:0001948 protein binding • syntaxin binding • protein kinase binding • cadherin binding • identical protein binding Cellular component • cytoplasm • Golgi apparatus • spindle pole • membrane • Golgi membrane • cis-Golgi network • Golgi cis cisterna • Golgi cisterna membrane • COPII-coated ER to Golgi transport vesicle • mitotic spindle • microtubule • endoplasmic reticulum-Golgi intermediate compartment membrane • cytoskeleton Biological process • positive regulation of protein glycosylation • negative regulation of protein binding • cell division • spindle assembly • protein glycosylation • ER to Golgi vesicle-mediated transport • mitotic spindle assembly • COPII vesicle coating • asymmetric cell division • cell cycle • Golgi disassembly • negative regulation of autophagy • protein homotetramerization • Golgi ribbon formation • microtubule nucleation • spindle assembly involved in meiosis • GO:0090623, GO:1903835, GO:0007092, GO:0051488 positive regulation of ubiquitin protein ligase activity • centrosome cycle • Golgi organization • protein transport Sources:Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	2801	99412
Ensembl	ENSG00000167110	ENSMUSG00000002546
UniProt	Q08379	Q921M4
RefSeq (mRNA)	NM_004486 NM_001366244 NM_001366246	NM_001080968 NM_133852 NM_001362695 NM_001362696 NM_001362697 NM_001362698
RefSeq (protein)	NP_004477 NP_001353173 NP_001353175	NP_001074437 NP_598613 NP_001349624 NP_001349625 NP_001349626 NP_001349627
Location (UCSC)	Chr 9: 128.26 – 128.28 Mb	Chr 2: 32.29 – 32.31 Mb
PubMed search	[3]	[4]
Wikidata
View/Edit Human View/Edit Mouse

Function

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. The golgins are a family of proteins, of which the protein encoded by this gene is a member, that are localized to the Golgi. This encoded protein has been postulated to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined.[6]

A patient with a neuromuscular disorder has been identified that is homozygous for a deletion mutation in this gene, and morpholino knockdown in zebrafish has shown similar phenotypes.[7]

Interactions

GOLGA2 has been shown to interact with:

• GORASP1,[8][9][10][11]
• GORASP2,[8][9][10]
• RAB1A,[8][12][13]
• RAB1B[14] and
• RAB2A.[8][15]
• WAC protein[16]

References

1. GRCh38: Ensembl release 89: ENSG00000167110 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000002546 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Fritzler MJ, Hamel JC, Ochs RL, Chan EK (Jul 1993). "Molecular characterization of two human autoantigens: unique cDNAs encoding 95- and 160-kD proteins of a putative family in the Golgi complex". The Journal of Experimental Medicine. 178 (1): 49–62. doi:10.1084/jem.178.1.49. PMC 2191081. PMID 8315394.
6. "Entrez Gene: GOLGA2 golgi autoantigen, golgin subfamily a, 2".
7. Shamseldin HE, Bennett AH, Alfadhel M, Gupta V, Alkuraya FS (2016). "GOLGA2, encoding a master regulator of golgi apparatus, is mutated in a patient with a neuromuscular disorder". Human Genetics. 135 (2): 245–51. doi:10.1007/s00439-015-1632-8. PMC 4975006. PMID 26742501.
8. Pfeffer SR (Dec 2001). "Constructing a Golgi complex". The Journal of Cell Biology. 155 (6): 873–5. doi:10.1083/jcb.200109095. PMC 2150916. PMID 11739400.
9. Shorter J, Watson R, Giannakou ME, Clarke M, Warren G, Barr FA (Sep 1999). "GRASP55, a second mammalian GRASP protein involved in the stacking of Golgi cisternae in a cell-free system". The EMBO Journal. 18 (18): 4949–60. doi:10.1093/emboj/18.18.4949. PMC 1171566. PMID 10487747.
10. Barr FA, Preisinger C, Kopajtich R, Körner R (Dec 2001). "Golgi matrix proteins interact with p24 cargo receptors and aid their efficient retention in the Golgi apparatus". The Journal of Cell Biology. 155 (6): 885–91. doi:10.1083/jcb.200108102. PMC 2150891. PMID 11739402.
11. Marra P, Maffucci T, Daniele T, Tullio GD, Ikehara Y, Chan EK, Luini A, Beznoussenko G, Mironov A, De Matteis MA (Dec 2001). "The GM130 and GRASP65 Golgi proteins cycle through and define a subdomain of the intermediate compartment". Nature Cell Biology. 3 (12): 1101–13. doi:10.1038/ncb1201-1101. PMID 11781572.
12. Weide T, Teuber J, Bayer M, Barnekow A (Jun 2003). "MICAL-1 isoforms, novel rab1 interacting proteins". Biochemical and Biophysical Research Communications. 306 (1): 79–86. doi:10.1016/s0006-291x(03)00918-5. PMID 12788069.
13. Valsdottir R, Hashimoto H, Ashman K, Koda T, Storrie B, Nilsson T (Nov 2001). "Identification of rabaptin-5, rabex-5, and GM130 as putative effectors of rab33b, a regulator of retrograde traffic between the Golgi apparatus and ER". FEBS Letters. 508 (2): 201–9. doi:10.1016/s0014-5793(01)02993-3. PMID 11718716.
14. Weide T, Bayer M, Köster M, Siebrasse JP, Peters R, Barnekow A (Apr 2001). "The Golgi matrix protein GM130: a specific interacting partner of the small GTPase rab1b". EMBO Reports. 2 (4): 336–41. doi:10.1093/embo-reports/kve065. PMC 1083862. PMID 11306556.
15. Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (Oct 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
16. Joachim J, Jefferies HB, Razi M, Frith D, Snijders AP, Chakravarty P, Judith D, Tooze SA (2015). "Activation of ULK Kinase and Autophagy by GABARAP Trafficking from the Centrosome Is Regulated by WAC and GM130". Molecular Cell. 60 (6): 899–913. doi:10.1016/j.molcel.2015.11.018. PMC 4691241. PMID 26687599.

Further reading

• Nakamura N, Rabouille C, Watson R, Nilsson T, Hui N, Slusarewicz P, Kreis TE, Warren G (Dec 1995). "Characterization of a cis-Golgi matrix protein, GM130". The Journal of Cell Biology. 131 (6 Pt 2): 1715–26. doi:10.1083/jcb.131.6.1715. PMC 2120691. PMID 8557739.
• Nakamura N, Lowe M, Levine TP, Rabouille C, Warren G (May 1997). "The vesicle docking protein p115 binds GM130, a cis-Golgi matrix protein, in a mitotically regulated manner". Cell. 89 (3): 445–55. doi:10.1016/S0092-8674(00)80225-1. PMID 9150144.
• Barr FA, Puype M, Vandekerckhove J, Warren G (Oct 1997). "GRASP65, a protein involved in the stacking of Golgi cisternae". Cell. 91 (2): 253–62. doi:10.1016/S0092-8674(00)80407-9. PMID 9346242.
• Sohda M, Misumi Y, Yano A, Takami N, Ikehara Y (Feb 1998). "Phosphorylation of the vesicle docking protein p115 regulates its association with the Golgi membrane". The Journal of Biological Chemistry. 273 (9): 5385–8. doi:10.1074/jbc.273.9.5385. PMID 9478999.
• Barr FA, Nakamura N, Warren G (Jun 1998). "Mapping the interaction between GRASP65 and GM130, components of a protein complex involved in the stacking of Golgi cisternae". The EMBO Journal. 17 (12): 3258–68. doi:10.1093/emboj/17.12.3258. PMC 1170664. PMID 9628863.
• Lowe M, Rabouille C, Nakamura N, Watson R, Jackman M, Jämsä E, Rahman D, Pappin DJ, Warren G (Sep 1998). "Cdc2 kinase directly phosphorylates the cis-Golgi matrix protein GM130 and is required for Golgi fragmentation in mitosis". Cell. 94 (6): 783–93. doi:10.1016/S0092-8674(00)81737-7. PMID 9753325.
• Barr FA (Apr 1999). "A novel Rab6-interacting domain defines a family of Golgi-targeted coiled-coil proteins". Current Biology. 9 (7): 381–4. doi:10.1016/S0960-9822(99)80167-5. PMID 10209123.
• Shorter J, Watson R, Giannakou ME, Clarke M, Warren G, Barr FA (Sep 1999). "GRASP55, a second mammalian GRASP protein involved in the stacking of Golgi cisternae in a cell-free system". The EMBO Journal. 18 (18): 4949–60. doi:10.1093/emboj/18.18.4949. PMC 1171566. PMID 10487747.
• Bouchireb N, Clark MS (1999). "Human golgin-95 gene (GOLGA2). Map position 9q32-34.1". Chromosome Research : An International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology. 7 (6): 501. doi:10.1023/A:1009210232273. PMID 10560974.
• Linstedt AD, Jesch SA, Mehta A, Lee TH, Garcia-Mata R, Nelson DS, Sztul E (Apr 2000). "Binding relationships of membrane tethering components. The giantin N terminus and the GM130 N terminus compete for binding to the p115 C terminus". The Journal of Biological Chemistry. 275 (14): 10196–201. doi:10.1074/jbc.275.14.10196. PMID 10744704.
• Lowe M, Gonatas NK, Warren G (Apr 2000). "The mitotic phosphorylation cycle of the cis-Golgi matrix protein GM130". The Journal of Cell Biology. 149 (2): 341–56. doi:10.1083/jcb.149.2.341. PMC 2175168. PMID 10769027.
• Alvarez C, Garcia-Mata R, Hauri HP, Sztul E (Jan 2001). "The p115-interactive proteins GM130 and giantin participate in endoplasmic reticulum-Golgi traffic". The Journal of Biological Chemistry. 276 (4): 2693–700. doi:10.1074/jbc.M007957200. PMID 11035033.
• Moyer BD, Allan BB, Balch WE (Apr 2001). "Rab1 interaction with a GM130 effector complex regulates COPII vesicle cis--Golgi tethering". Traffic. 2 (4): 268–76. doi:10.1034/j.1600-0854.2001.1o007.x. PMID 11285137.
• Weide T, Bayer M, Köster M, Siebrasse JP, Peters R, Barnekow A (Apr 2001). "The Golgi matrix protein GM130: a specific interacting partner of the small GTPase rab1b". EMBO Reports. 2 (4): 336–41. doi:10.1093/embo-reports/kve065. PMC 1083862. PMID 11306556.
• Puthenveedu MA, Linstedt AD (Oct 2001). "Evidence that Golgi structure depends on a p115 activity that is independent of the vesicle tether components giantin and GM130". The Journal of Cell Biology. 155 (2): 227–38. doi:10.1083/jcb.200105005. PMC 2198842. PMID 11591729.
• Miles S, McManus H, Forsten KE, Storrie B (Nov 2001). "Evidence that the entire Golgi apparatus cycles in interphase HeLa cells: sensitivity of Golgi matrix proteins to an ER exit block". The Journal of Cell Biology. 155 (4): 543–55. doi:10.1083/jcb.200103104. PMC 2198853. PMID 11696556.
• Majoul I, Straub M, Hell SW, Duden R, Söling HD (Jul 2001). "KDEL-cargo regulates interactions between proteins involved in COPI vesicle traffic: measurements in living cells using FRET". Developmental Cell. 1 (1): 139–53. doi:10.1016/S1534-5807(01)00004-1. PMID 11703931.
• Valsdottir R, Hashimoto H, Ashman K, Koda T, Storrie B, Nilsson T (Nov 2001). "Identification of rabaptin-5, rabex-5, and GM130 as putative effectors of rab33b, a regulator of retrograde traffic between the Golgi apparatus and ER". FEBS Letters. 508 (2): 201–9. doi:10.1016/S0014-5793(01)02993-3. PMID 11718716.
• Short B, Preisinger C, Körner R, Kopajtich R, Byron O, Barr FA (Dec 2001). "A GRASP55-rab2 effector complex linking Golgi structure to membrane traffic". The Journal of Cell Biology. 155 (6): 877–83. doi:10.1083/jcb.200108079. PMC 2150909. PMID 11739401.