GRASP65

Golgi reassembly-stacking protein of 65 kDa (GRASP65) also known as Golgi reassembly-stacking protein 1 (GORASP1) is a protein that in humans is encoded by the GORASP1 gene.[4]

GORASP1
Available structures
PDBOrtholog search: PDBe RCSB
Identifiers
AliasesGORASP1, GOLPH5, GRASP65, P65, golgi reassembly stacking protein 1
External IDsOMIM: 606867 MGI: 1921748 HomoloGene: 49916 GeneCards: GORASP1
Gene location (Human)
Chr.Chromosome 3 (human)[1]
Band3p22.2Start39,096,659 bp[1]
End39,108,363 bp[1]
RNA expression pattern
More reference expression data
Orthologs
SpeciesHumanMouse
Entrez

64689

74498

Ensembl

ENSG00000114745

n/a

UniProt

Q9BQQ3

Q91X51

RefSeq (mRNA)

NM_001278789
NM_001278790
NM_031899

NM_028976

RefSeq (protein)

NP_001265718
NP_001265719
NP_114105
NP_114105.1

NP_083252

Location (UCSC)Chr 3: 39.1 – 39.11 Mbn/a
PubMed search[2][3]
Wikidata
View/Edit HumanView/Edit Mouse

Function
Microinjection of antibodies to GRASP65 prevents normal Golgi stack formation.[5]

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The GRASP65 protein is a peripheral membrane protein anchored to the lipid bilayer through myristoylation of a glycine residue near the protein's amino terminus.[6] It is involved in establishing the stacked structure of the Golgi apparatus and linking the stacks into larger ribbons in vertebrate cells.[6] It is a caspase-3 substrate, and cleavage of this encoded protein contributes to Golgi fragmentation in apoptosis.[7][8] GRASP65 can form a complex with the Golgi matrix protein GM130, and this complex binds to the vesicle docking protein p115.[6][lower-alpha 1] Several alternatively spliced transcript variants of this gene have been identified, but their full-length natures have not been determined.[4]

Structure

GRASP65 contains two PDZ domains in the amino-terminal GRASP domain (amino acid residues 2–210), that comprises approximately half of the protein. The GRASP region interacts with the Golgi matrix protein GM130 as well as an intrinsically disordered region in the C-terminus.[6][9]

  • GRASP65 superhelix, topview[6]
  • GRASP65 superhelix, side view[6]

Interactions

GORASP1 has been shown to interact with TGF alpha,[10] TMED2[10] and GOLGA2.[10][11][12]

Notes
  1. This is shown in the external link entitled "Molecular models of GRASP65/GM130/P115-mediated cis-cisternae membrane stacking and vesicle tethering."
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References
  1. GRCh38: Ensembl release 89: ENSG00000114745 - Ensembl, May 2017
  2. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  3. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. "Entrez Gene: GORASP1 golgi reassembly stacking protein 1, 65kDa".
  5. Wang Y, Wei JH, Bisel B, Tang D, Seemann J (February 2008). "Golgi cisternal unstacking stimulates COPI vesicle budding and protein transport". PLoS One. 3 (2): e1647. doi:10.1371/journal.pone.0001647. PMC 2249924. PMID 18297130.
  6. Hu F, Shi X, Li B, Huang X, Morelli X, Shi N (2015). "Structural basis for the interaction between the Golgi reassembly-stacking protein GRASP65 and the Golgi matrix protein GM130". The Journal of Biological Chemistry. 290 (44): 26373–82. doi:10.1074/jbc.M115.657940. PMC 4646294. PMID 26363069.
  7. Lane JD, Lucocq J, Pryde J, Barr FA, Woodman PG, Allan VJ, Lowe M (2002). "Caspase-mediated cleavage of the stacking protein GRASP65 is required for Golgi fragmentation during apoptosis". The Journal of Cell Biology. 156 (3): 495–509. doi:10.1083/jcb.200110007. PMC 2173349. PMID 11815631.
  8. Cheng JP, Betin VM, Weir H, Shelmani GM, Moss DK, Lane JD (2010). "Caspase cleavage of the Golgi stacking factor GRASP65 is required for Fas/CD95-mediated apoptosis". Cell Death & Disease. 1 (10): e82. doi:10.1038/cddis.2010.59. PMC 3035901. PMID 21368855.
  9. Rabouille C, Linstedt AD (2016). "GRASP: A Multitasking Tether". Frontiers in Cell and Developmental Biology. 4: 1. doi:10.3389/fcell.2016.00001. PMC 4726779. PMID 26858948.
  10. Barr FA, Preisinger C, Kopajtich R, Körner R (December 2001). "Golgi matrix proteins interact with p24 cargo receptors and aid their efficient retention in the Golgi apparatus". The Journal of Cell Biology. 155 (6): 885–91. doi:10.1083/jcb.200108102. PMC 2150891. PMID 11739402.
  11. Short B, Preisinger C, Körner R, Kopajtich R, Byron O, Barr FA (December 2001). "A GRASP55-rab2 effector complex linking Golgi structure to membrane traffic". The Journal of Cell Biology. 155 (6): 877–83. doi:10.1083/jcb.200108079. PMC 2150909. PMID 11739401.
  12. Shorter J, Watson R, Giannakou ME, Clarke M, Warren G, Barr FA (September 1999). "GRASP55, a second mammalian GRASP protein involved in the stacking of Golgi cisternae in a cell-free system". The EMBO Journal. 18 (18): 4949–60. doi:10.1093/emboj/18.18.4949. PMC 1171566. PMID 10487747.

Further reading


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