Farrell's medium

Farrell's medium is a selective bacteriological medium for Brucella species which is prepared by the addition of six antibiotics to a basal bacteriological medium such as serum dextrose agar. In order to prepare 1 liter of the Farrell's medium, the following quantities are added to 1 liter of serum dextrose agar: polymyxin B sulfate (5000 units =5 mg), bacitracin (25,000 units = 25 mg), natamycin (50 mg), nalidixic acid (5 mg), nystatin (100,000 units), and vancomycin (20 mg). Vancomycin inhibits the growth of gram-positive bacteria on this medium, while nystatin inhibits the growth of fungi. Other antibiotics inhibit the growth of gram-negative bacteria other than Brucella species, thus favoring the exclusive growth of the latter in this medium.[1]

References
  1. "Archived copy" (PDF). Archived from the original (PDF) on 2015-06-06. Retrieved 2015-12-29.CS1 maint: archived copy as title (link)
This article is issued from Wikipedia. The text is licensed under Creative Commons - Attribution - Sharealike. Additional terms may apply for the media files.